TATD1_MOUSE
ID TATD1_MOUSE Reviewed; 295 AA.
AC Q6P8M1; Q8BY37;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Deoxyribonuclease TATDN1;
DE EC=3.1.21.-;
GN Name=Tatdn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-27 AND LYS-46, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Deoxyribonuclease which catalyzes (in vitro) the decatenation
CC of kinetoplast DNA, which are circular DNA catenated to each other,
CC producing linear DNA molecules (By similarity). Plays an important role
CC in chromosomal segregation and cell cycle progression during eye
CC development probably via its DNA decatenation activity (By similarity).
CC {ECO:0000250|UniProtKB:Q6GML7}.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250|UniProtKB:Q17R31};
CC Note=Binds 2 divalent metal cations per subunit.
CC {ECO:0000250|UniProtKB:Q17R31};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P8M1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P8M1-2; Sequence=VSP_030046, VSP_030047;
CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC TatD-type hydrolase family. {ECO:0000305}.
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DR EMBL; AK042248; BAC31203.1; -; mRNA.
DR EMBL; BC061187; AAH61187.1; -; mRNA.
DR CCDS; CCDS88768.1; -. [Q6P8M1-1]
DR RefSeq; NP_780360.1; NM_175151.4.
DR RefSeq; XP_006521428.1; XM_006521365.3.
DR AlphaFoldDB; Q6P8M1; -.
DR SMR; Q6P8M1; -.
DR BioGRID; 213619; 9.
DR STRING; 10090.ENSMUSP00000105783; -.
DR iPTMnet; Q6P8M1; -.
DR PhosphoSitePlus; Q6P8M1; -.
DR EPD; Q6P8M1; -.
DR MaxQB; Q6P8M1; -.
DR PaxDb; Q6P8M1; -.
DR PeptideAtlas; Q6P8M1; -.
DR PRIDE; Q6P8M1; -.
DR ProteomicsDB; 254817; -. [Q6P8M1-1]
DR ProteomicsDB; 254818; -. [Q6P8M1-2]
DR Antibodypedia; 13907; 95 antibodies from 19 providers.
DR DNASU; 69694; -.
DR Ensembl; ENSMUST00000228538; ENSMUSP00000154677; ENSMUSG00000050891. [Q6P8M1-1]
DR GeneID; 69694; -.
DR KEGG; mmu:69694; -.
DR UCSC; uc007vtr.1; mouse. [Q6P8M1-2]
DR UCSC; uc007vtt.1; mouse. [Q6P8M1-1]
DR CTD; 83940; -.
DR MGI; MGI:1916944; Tatdn1.
DR VEuPathDB; HostDB:ENSMUSG00000050891; -.
DR eggNOG; KOG3020; Eukaryota.
DR GeneTree; ENSGT00940000156272; -.
DR InParanoid; Q6P8M1; -.
DR OMA; PNEAPRI; -.
DR OrthoDB; 897451at2759; -.
DR PhylomeDB; Q6P8M1; -.
DR TreeFam; TF324192; -.
DR BioGRID-ORCS; 69694; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Tatdn1; mouse.
DR PRO; PR:Q6P8M1; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q6P8M1; protein.
DR Bgee; ENSMUSG00000050891; Expressed in knee joint and 226 other tissues.
DR ExpressionAtlas; Q6P8M1; baseline and differential.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0008296; F:3'-5'-exodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01310; TatD_DNAse; 1.
DR InterPro; IPR018228; DNase_TatD-rel_CS.
DR InterPro; IPR032466; Metal_Hydrolase.
DR InterPro; IPR001130; TatD-like.
DR Pfam; PF01026; TatD_DNase; 1.
DR PIRSF; PIRSF005902; DNase_TatD; 1.
DR SUPFAM; SSF51556; SSF51556; 1.
DR PROSITE; PS01091; TATD_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Metal-binding; Nuclease; Nucleus;
KW Reference proteome.
FT CHAIN 1..295
FT /note="Deoxyribonuclease TATDN1"
FT /id="PRO_0000313591"
FT BINDING 112
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q17R31"
FT BINDING 112
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q17R31"
FT BINDING 149
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q17R31"
FT BINDING 174
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q17R31"
FT BINDING 222
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q17R31"
FT MOD_RES 27
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 46
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT VAR_SEQ 264
FT /note="I -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030046"
FT VAR_SEQ 265
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030047"
SQ SEQUENCE 295 AA; 33365 MW; 2731F702DBE57CB8 CRC64;
MSLFKFVDIG INLTDPMFRG IYRGVQKHQD DLQDVIERAI QIGVKKFMIT GGSLQDSKDA
LQLAQTNDMF FSTVGCHPTR CDEFEKGSPD QYLAGLLSLA ENNKGKVVAI GECGLDFDRL
QFCPKDTQLK YFEKQFELSE QTQLPMFLHC RNSHTEFLDI MRRNRDRYVG GVVHSFDGTK
EAAAALVDLG LYIGFNGCSL KTEANLEVLK SIPSEKLMIE TDAPWCGVKS THAGSKYINT
SFPTKKKWEN GHCLKDRNEP CHIIQILEIM SAVREEDPLE LANTLYNNTI KVFFS
