ID   TATD1_NOSCE             Reviewed;         259 AA.
AC   C4VAS3;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Putative deoxyribonuclease TATDN1 homolog;
DE            EC=3.1.21.-;
GN   ORFNames=NCER_101798;
OS   Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX   NCBI_TaxID=578460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRL01;
RX   PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA   Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA   Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT   "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT   pathogen of honey bees.";
RL   PLoS Pathog. 5:E1000466-E1000466(2009).
CC   -!- FUNCTION: Putative deoxyribonuclease. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC       Note=Binds 2 divalent metal cations per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       TatD-type hydrolase family. {ECO:0000305}.
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DR   EMBL; ACOL01000287; EEQ81679.1; -; Genomic_DNA.
DR   RefSeq; XP_002995350.1; XM_002995304.1.
DR   AlphaFoldDB; C4VAS3; -.
DR   SMR; C4VAS3; -.
DR   STRING; 578460.C4VAS3; -.
DR   EnsemblFungi; EEQ81679; EEQ81679; NCER_101798.
DR   KEGG; nce:NCER_101798; -.
DR   VEuPathDB; MicrosporidiaDB:NCER_101798; -.
DR   HOGENOM; CLU_031506_1_1_1; -.
DR   InParanoid; C4VAS3; -.
DR   OMA; DIAFNIT; -.
DR   Proteomes; UP000009082; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   CDD; cd01310; TatD_DNAse; 1.
DR   InterPro; IPR018228; DNase_TatD-rel_CS.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR001130; TatD-like.
DR   Pfam; PF01026; TatD_DNase; 1.
DR   PIRSF; PIRSF005902; DNase_TatD; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   PROSITE; PS01091; TATD_3; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..259
FT                   /note="Putative deoxyribonuclease TATDN1 homolog"
FT                   /id="PRO_0000388427"
FT   BINDING         82
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         82
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         116
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         138
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         186
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   259 AA;  29949 MW;  A7B6D18147F3F64E CRC64;
     MIVDISCNIT DSQFKNVEDT ILKCKEAKVI PIFVGVDYTT SLKSCNYAEK FDTLFYAGIH
     PLYSENSVYI PVNSDRCIAI GECGLDYYRL QFSSIETQKR IFKVQLDLKA ERYFLHCRDS
     HRDFMEILSD YNFKGVVHSF TGTVEESKEI IKKGLFIGIN GCSLKNEEGI EVVKNLPLNS
     ILVETDSPYC KIRKSYAGYK FVNNYQKHKS NEPYLIYEII EAIANIKNIP INILLDTLLS
     NNINFYGERL VECFEKWRL