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TAZB_ASPTN
ID   TAZB_ASPTN              Reviewed;        2620 AA.
AC   Q0CS88;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Highly reducing polyketide synthase tazB {ECO:0000303|PubMed:35398258};
DE            Short=HR-PKS azaB {ECO:0000303|PubMed:35398258};
DE            EC=2.3.1.- {ECO:0000305|PubMed:35398258};
DE   AltName: Full=Azaphilone biosynthesis cluster protein B {ECO:0000303|PubMed:35398258};
GN   Name=tazB {ECO:0000303|PubMed:35398258}; ORFNames=ATEG_03446;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=35398258; DOI=10.1016/j.fgb.2022.103694;
RA   Sun W.W., Li C.Y., Chiang Y.M., Lin T.S., Warren S., Chang F.R.,
RA   Wang C.C.C.;
RT   "Characterization of a silent azaphilone biosynthesis gene cluster in
RT   Aspergillus terreus NIH 2624.";
RL   Fungal Genet. Biol. 160:103694-103694(2022).
CC   -!- FUNCTION: Highly reducing polyketide synthase; part of the gene cluster
CC       that mediates the biosynthesis of azaterrilone A and other azaphilones,
CC       a class of fungal metabolites characterized by a highly oxygenated
CC       pyrano-quinone bicyclic core and exhibiting a broad range of
CC       bioactivities (PubMed:35398258). The first step of the pathway begins
CC       with the non-reducing polyketide synthase tazA that assembles one
CC       acetyl-CoA starter unit, five malonyl-CoA units, and catalyzes a series
CC       of Claisen condensations, methylation, PT-mediated cyclization, and
CC       finally releases the first hexaketide precursor through the R-domain.
CC       The tazA product then undergoes reduction on its terminal ketone and
CC       the following pyran-ring formation by yet undetermined enzyme(s).
CC       Dehydration and enoyl reduction, possibly involving the trans-enoyl
CC       reductase tazE leads to the next intermediate. TazD is predicted as an
CC       acetyltransferase and might catalyze the acetylation steps leading to
CC       the synthesis of azaterrilone A. Azaterrilone A is not the final
CC       product of the taz pathway and both the highly reducing polyketide
CC       synthase tazB and the dual enzyme tazHJ catalyze late steps of the
CC       pathway, leading to the production of the 2 final stereoisomers that
CC       contain additional polyketide modification whose structures have still
CC       to be determined (Probable). {ECO:0000269|PubMed:35398258,
CC       ECO:0000305|PubMed:35398258}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:35398258}.
CC   -!- INDUCTION: Expression is positively regulated by the azaterrilone A
CC       cluster-specific transcription factor tazR.
CC       {ECO:0000269|PubMed:35398258}.
CC   -!- DOMAIN: Multidomain protein; including a ketosynthase (KS) that
CC       catalyzes repeated decarboxylative condensation to elongate the
CC       polyketide backbone; a malonyl-CoA:ACP transacylase (MAT) that selects
CC       and transfers the extender unit malonyl-CoA; a dehydratase (DH) domain
CC       that reduces hydroxyl groups to enoyl groups; a methyltransferase
CC       (CMeT) domain responsible for the incorporation of methyl groups; an
CC       enoylreductase (ER) domain that reduces enoyl groups to alkyl group; a
CC       ketoreductase (KR) domain that catalyzes beta-ketoreduction steps; and
CC       an acyl-carrier protein (ACP) that serves as the tether of the growing
CC       and completed polyketide via its phosphopantetheinyl arm.
CC       {ECO:0000305|PubMed:35398258}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect the production of azaterrilone A
CC       but impairs the production of the 2 final isomers.
CC       {ECO:0000269|PubMed:35398258}.
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DR   EMBL; CH476597; EAU36720.1; -; Genomic_DNA.
DR   RefSeq; XP_001212624.1; XM_001212624.1.
DR   STRING; 33178.CADATEAP00006812; -.
DR   EnsemblFungi; EAU36720; EAU36720; ATEG_03446.
DR   GeneID; 4317679; -.
DR   VEuPathDB; FungiDB:ATEG_03446; -.
DR   eggNOG; KOG1202; Eukaryota.
DR   HOGENOM; CLU_000022_31_0_1; -.
DR   OMA; LVAMEFR; -.
DR   OrthoDB; 19161at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR   Gene3D; 3.10.129.110; -; 1.
DR   Gene3D; 3.40.366.10; -; 1.
DR   Gene3D; 3.40.47.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR001227; Ac_transferase_dom_sf.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR014043; Acyl_transferase.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR013149; ADH-like_C.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR020807; PKS_dehydratase.
DR   InterPro; IPR042104; PKS_dehydratase_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR016039; Thiolase-like.
DR   Pfam; PF00698; Acyl_transf_1; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   Pfam; PF08659; KR; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   Pfam; PF14765; PS-DH; 1.
DR   SMART; SM00827; PKS_AT; 1.
DR   SMART; SM00826; PKS_DH; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 2.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53901; SSF53901; 1.
DR   SUPFAM; SSF55048; SSF55048; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Metal-binding; Multifunctional enzyme; NADP;
KW   Oxidoreductase; Phosphopantetheine; Phosphoprotein; Reference proteome;
KW   Transferase; Zinc.
FT   CHAIN           1..2620
FT                   /note="Highly reducing polyketide synthase tazB"
FT                   /id="PRO_0000456065"
FT   DOMAIN          2539..2620
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   REGION          26..419
FT                   /note="Ketosynthase (KS) domain"
FT                   /evidence="ECO:0000255"
FT   REGION          601..923
FT                   /note="Malonyl-CoA:ACP transacylase (MAT) domain"
FT                   /evidence="ECO:0000255"
FT   REGION          993..1313
FT                   /note="Dehydrogenase (DH) domain"
FT                   /evidence="ECO:0000255"
FT   REGION          1379..1680
FT                   /note="Methyltransferase (CMet) domain"
FT                   /evidence="ECO:0000255"
FT   REGION          1910..2227
FT                   /note="Enoyl reductase (ER) domain"
FT                   /evidence="ECO:0000255"
FT   REGION          2251..2425
FT                   /note="Ketoreductase (KR) domain"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        166
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT   MOD_RES         2576
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ   SEQUENCE   2620 AA;  287510 MW;  B9E3139B43D3A125 CRC64;
     MPFLNGNTTH HEAHSAEPDH GNTEPMVIIG LAMRAADEAT DAEAFWDFLF YVKGAAFLEE
     SPNGFDAAFF KMSKTEVQSL DPQQRILMEN VYHALENAGL PMEDVISSNT SVFVSGFNYH
     HADRLNSDLE LSFKHRPTGA ENSMISGRVS WFYDFQGASL TIDTACSSSL VGLHLARQSL
     QAKESDMAIV SGVSVIGYLS HLMKMSYSGL LGSEGKSLAF DQRADGYGLG EGVGTVILKT
     LSAAIRDGDT IRAVVRGTGL NHDGHTPGMT YPSAAAQESL IRKTYVAAGL DPKDTIYAES
     HGTGTQAGDL MECTAIAAAF ETEKRDRPFY IGAVKPNVGH LEGGAGITSV IKSVLLLESG
     IIPPNATLKK INPKIRPEWN LQFPTRCVPW PTTGIRRSSI SSYGISGTNA HCILDDAYHY
     CNQRGISVRH RTAETVPSEK EIELIVAKAV RRYQTNSANG FNKFDEPRGS DSAGSNANGS
     HGVAGTVGAN GNGVNGINPS PGHMPENTKA LQASSLLLFS AFDEKSLEKV LAQIRNYISS
     LDSNSAGQAL HDLAFTLSTR RSRLPWKTYA LCSSLVELHD MLSKPHLKAI KSRSPLRVGF
     VFTGQGAQYA QMGQQLLLYP VFRQSLEEAS LYFKSLGCDW SLLEELTRDS KDSRISKSAF
     AHPLSCAVQI ALLDLLLSWN VVPHRVVGHS SGEIAAAYCA GKISREGAWR VAYYRGHVLL
     HGKTDRVGGM LAAGIEEEPL LDLLSQVHAA LPGGTLSIAC YNSPRNHTIS GDDAMVDALK
     VLLDEQGIFT RKLKVEHAAH SAHMEQFTGE YEELVGDLPS NRLLHFDHTV HMFSTLTGRL
     IDDSCVPEKS SHWSNSMVGP VEFTKAVSSM CFDSILGDGS SVQGSQVDVI LEVGPHPAMQ
     SAVKDILGLG SGIPYLATLS RKDTGLGTLL DTIGSLAAHG APVDLDKVNR SANPLLKPRM
     LADLPPYPFS HEEQGLYESR LIKNIRLRQF PRHDLFGAPV PDWNPNSPRW RHFLRVSENP
     WLKEHVIGDE FVFPGAGYMV MAVEGMKQIT DPAEMVGIFL RDVKFKAMLV VPDDTQGVEV
     CLSFYPVPES RTSLSTAWKR FEVASYNQKT EEWIEHCTGE VSPDLKKSLN PIDGTRTQEA
     EKAQFSAFAQ SRQDVCTAPV DFTPVYDHLR KIGVNHGPSF RNLAAVNIGD REQGLMTGDI
     VVPDITQVMP EKYAHGHLIH PTTLDNAFHA SFASIYDLEG KTMMRRGCVP SYVQDVWLSA
     TALSSDPGTI LRCTSEASHA LHGAFESTVH AWDPANPSER LISLAGIRLS PFKPESSESI
     AAENRTCYSV EWYPDLNLLT KREFQKLLSR FPAPLETFDA QQKWFSQLQL ASTLLATDGL
     RESREMKDVN LEDHQRGYRE LLRAIAAGVT TQSIPYVSLD MWLEYSRNSD LKEQLYREIE
     DQSPDGALLV RMGAAIPSIL GREITAQYLL YEQDDLLSVW DENRLSRGKI LPALTQYLTL
     LRKSQRGLRV LELGSRTGVL AEHVLKTLCV DGTENSIEQY TIRSQSADHC EKLKKRLSAW
     VDIIRYEALD LTAKSSEQEI QINPFDLVIV NNFVRGQPNM EEMMLRLNSL MRQGGRLLIL
     EDVRSESLHA NIIFGALPGW REATKASWDA ESGTDKLEWD RVLRNTGFSG VDFEASSSIY
     PDFADFSLVA STASHGMLEA TPPSFEVLVI IQSHSEISRS LARGLLTAGV SHSICLIDDI
     RADNVIGRVC ISLLEAEQPI LNSMDETTFQ AIQNLVTACD SLLWVTGDPL AHPEFQMATG
     LIRTIRWELD RNDLNLITIA LDGESTASTD MNVDALIRVL RYQFLDQCSK DSGNTNSEYR
     IRDSVIETNH AVKNTVASAV IEAQFSSPKP TLSTWESIER PVRLINTSPG IDSLTWVTDE
     DISRKPLAVN EIEIDVHAVG LNFKDLLVAM GEIDQPGFGH EAAGIVVRVG SSVSTFKAGD
     RVMYLGDPSP GKMGTLRTRS RVHCGLAIKI PDTMGFEIAA GLPIIYGTVI YSLGHIARLR
     AGEKVLIHAA AGGIGQAAIQ YAHAKGAEIF VTLSSLEKKQ YIMENFHIRP DHIFSSRDLN
     FAAGIKRIAP AGVDVVLNSL SGEALRQSWQ CVAPFGRFVE IGKLDLQAGS KLDMTPFLYN
     VSFSGVDLNA LAENRPEVCQ ELLQETIDLW SNQDIHEARP TQVLDYGQLK EGLRLLQTGK
     SIGKVTLVPG THPVSVIPPP FLPLELDANA SFILAGGLGG IGRSIALRLA RRGAKHIVFL
     SRSATVHEAG QETIAKLKLL GCTSHVFQCD ISNETRLLEV ITRVRETLPP IKGCIQCSFV
     LKDKAFDSMT HEEWQTALTP KVSGSWNLHC LLPDVDFFLL LSSITGIVGN RSQANYNAGN
     NFQDSLARYR VSKGMHGASV NLGAVVGIGF IAENAEYAAK HTFKMANPQT EEEVLATVEY
     LIDRRHHMAL SPDTAQLICG LRTPASYSLS NEAPPTHLKY PMFAQLPPAL SNTGPGGSHS
     AQSATHIRDQ LQSATAPEEA ARIIHKALRR KMADLLNISE DTIDDSLNVR ANGVDSLIEM
     EFRTWFAKEL GATVPLKDLA KDLTQLSARL VSLSSFTKFR
 
 
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