ID   TAZC_ASPTN              Reviewed;         265 AA.
AC   Q0CS97;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Probable esterase tazC {ECO:0000303|PubMed:35398258};
DE            EC=3.1.2.- {ECO:0000305|PubMed:35398258};
DE   AltName: Full=Azaphilone biosynthesis cluster protein C {ECO:0000303|PubMed:35398258};
GN   Name=tazC {ECO:0000303|PubMed:35398258}; ORFNames=ATEG_03437;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=35398258; DOI=10.1016/j.fgb.2022.103694;
RA   Sun W.W., Li C.Y., Chiang Y.M., Lin T.S., Warren S., Chang F.R.,
RA   Wang C.C.C.;
RT   "Characterization of a silent azaphilone biosynthesis gene cluster in
RT   Aspergillus terreus NIH 2624.";
RL   Fungal Genet. Biol. 160:103694-103694(2022).
CC   -!- FUNCTION: Probable esterase; part of the gene cluster that mediates the
CC       biosynthesis of azaterrilone A and other azaphilones, a class of fungal
CC       metabolites characterized by a highly oxygenated pyrano-quinone
CC       bicyclic core and exhibiting a broad range of bioactivities
CC       (PubMed:35398258). The first step of the pathway begins with the non-
CC       reducing polyketide synthase tazA that assembles one acetyl-CoA starter
CC       unit, five malonyl-CoA units, and catalyzes a series of Claisen
CC       condensations, methylation, PT-mediated cyclization, and finally
CC       releases the first hexaketide precursor through the R-domain. The tazA
CC       product then undergoes reduction on its terminal ketone and the
CC       following pyran-ring formation by yet undetermined enzyme(s).
CC       Dehydration and enoyl reduction, possibly involving the trans-enoyl
CC       reductase tazE leads to the next intermediate. TazD is predicted as an
CC       acetyltransferase and might catalyze the acetylation steps leading to
CC       the synthesis of azaterrilone A. Azaterrilone A is not the final
CC       product of the taz pathway and both the highly reducing polyketide
CC       synthase tazB and the dual enzyme tazHJ catalyze late steps of the
CC       pathway, leading to the production of the 2 final stereoisomers that
CC       contain additional polyketide modification whose structures have still
CC       to be determined (Probable). {ECO:0000269|PubMed:35398258,
CC       ECO:0000305|PubMed:35398258}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:35398258}.
CC   -!- INDUCTION: Expression is positively regulated by the azaterrilone A
CC       cluster-specific transcription factor tazR.
CC       {ECO:0000269|PubMed:35398258}.
CC   -!- SIMILARITY: Belongs to the LovG family. {ECO:0000305}.
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DR   EMBL; CH476597; EAU36711.1; -; Genomic_DNA.
DR   RefSeq; XP_001212615.1; XM_001212615.1.
DR   EnsemblFungi; EAU36711; EAU36711; ATEG_03437.
DR   GeneID; 4318032; -.
DR   VEuPathDB; FungiDB:ATEG_03437; -.
DR   eggNOG; KOG2551; Eukaryota.
DR   HOGENOM; CLU_051938_0_2_1; -.
DR   OMA; WDGGHRI; -.
DR   OrthoDB; 1190789at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005645; FSH_dom.
DR   Pfam; PF03959; FSH1; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..265
FT                   /note="Probable esterase tazC"
FT                   /id="PRO_0000456069"
FT   ACT_SITE        119
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        209
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
FT   ACT_SITE        236
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P38777"
SQ   SEQUENCE   265 AA;  29509 MW;  FFE1255DB2E93D9E CRC64;
     MRATIDDPQA ALPRILCLHG GGSNSRIFKA QTRALRYELS NYFRFVFVEG PFDSEPGPDV
     TSAYEKFGPF RRWFRSGPQH PPIDSHTAVA CIEESIYAAM ERDDLQGGTG DYVAVMGFSQ
     GAKLAASLLF RQQIRAEKLG QAMSGTNFRF AILLNGRGPL VSMDPELVMN TALMDASQIA
     LDRPVTLEDL CRKEHVLRLP TIHVHGLQDP GLMLHRVLLR EYCSKNARLV EWDGAHRVPI
     KTKDVLPLVH EILQLAKQTG VVFAA