ID   TAZD_ASPTN              Reviewed;         453 AA.
AC   Q0CS99;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Probable acetyltransferase tazD {ECO:0000303|PubMed:35398258};
DE            EC=2.3.2.- {ECO:0000305|PubMed:35398258};
DE   AltName: Full=Azaphilone biosynthesis cluster protein D {ECO:0000303|PubMed:35398258};
GN   Name=tazD {ECO:0000303|PubMed:35398258}; ORFNames=ATEG_03435;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=35398258; DOI=10.1016/j.fgb.2022.103694;
RA   Sun W.W., Li C.Y., Chiang Y.M., Lin T.S., Warren S., Chang F.R.,
RA   Wang C.C.C.;
RT   "Characterization of a silent azaphilone biosynthesis gene cluster in
RT   Aspergillus terreus NIH 2624.";
RL   Fungal Genet. Biol. 160:103694-103694(2022).
CC   -!- FUNCTION: Highly reducing polyketide synthase; part of the gene cluster
CC       that mediates the biosynthesis of azaterrilone A and other azaphilones,
CC       a class of fungal metabolites characterized by a highly oxygenated
CC       pyrano-quinone bicyclic core and exhibiting a broad range of
CC       bioactivities (PubMed:35398258). The first step of the pathway begins
CC       with the non-reducing polyketide synthase tazA that assembles one
CC       acetyl-CoA starter unit, five malonyl-CoA units, and catalyzes a series
CC       of Claisen condensations, methylation, PT-mediated cyclization, and
CC       finally releases the first hexaketide precursor through the R-domain.
CC       The tazA product then undergoes reduction on its terminal ketone and
CC       the following pyran-ring formation by yet undetermined enzyme(s).
CC       Dehydration and enoyl reduction, possibly involving the trans-enoyl
CC       reductase tazE leads to the next intermediate. TazD is predicted as an
CC       acetyltransferase and might catalyze the acetylation steps leading to
CC       the synthesis of azaterrilone A. Azaterrilone A is not the final
CC       product of the taz pathway and both the highly reducing polyketide
CC       synthase tazB and the dual enzyme tazHJ catalyze late steps of the
CC       pathway, leading to the production of the 2 final stereoisomers that
CC       contain additional polyketide modification whose structures have still
CC       to be determined (Probable). {ECO:0000269|PubMed:35398258,
CC       ECO:0000305|PubMed:35398258}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:35398258}.
CC   -!- INDUCTION: Expression is positively regulated by the azaterrilone A
CC       cluster-specific transcription factor tazR.
CC       {ECO:0000269|PubMed:35398258}.
CC   -!- SIMILARITY: Belongs to the trichothecene O-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; CH476597; EAU36709.1; -; Genomic_DNA.
DR   RefSeq; XP_001212613.1; XM_001212613.1.
DR   EnsemblFungi; EAU36709; EAU36709; ATEG_03435.
DR   GeneID; 4318030; -.
DR   VEuPathDB; FungiDB:ATEG_03435; -.
DR   eggNOG; ENOG502RB46; Eukaryota.
DR   HOGENOM; CLU_029138_1_0_1; -.
DR   OMA; VWTFRDQ; -.
DR   OrthoDB; 542619at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   Gene3D; 3.30.559.10; -; 1.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transferase.
FT   CHAIN           1..453
FT                   /note="Probable acetyltransferase tazD"
FT                   /id="PRO_0000456067"
SQ   SEQUENCE   453 AA;  50202 MW;  F70B0DE475339C4C CRC64;
     MPWIETKPGL WQRPIGDNEK FIKFIGDRSH QVGREHWSVT AGAAFTLNRD WSLSDLSSHC
     LNAWCAFRFD HPSIACTVEG DTVSYQTPSN EELASWANET FHIHDLSTTS DDLIAALKPS
     RYLTAHLLPG ENRVILHAAH WRTDGYGALQ LVHAFLDTLC KAVGCHSKAI QWGEEWRRLT
     PSIEEVLDLP LTATPEILEA TKECLKTLAH TRGAVGTAPS GDKLAAPAGT RSAHLSLTTA
     DTSAVMAACA ERKISLLSAV HASCAALTWL EASSNDRDKH YTSTMRFSLR PHLPPPYSGP
     AYAAGLYTGG YMFPVPASQS WIENAKQYEK EYNTGITEDF LKCRRQYAVE LIGILQRGAP
     PPDPPPSEVD ISSVGDAEAL VSPRFTHNGT ILEVQSVDIG VETLTRQTYC FLWTFRGRLE
     LHLVYNEAFY EPERAERMVR ILAKTLQTEL GLA