ID   TAZM_ASPTN              Reviewed;         413 AA.
AC   Q0CSA0;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Azaphilone biosynthesis cluster protein M {ECO:0000303|PubMed:35398258};
GN   Name=tazM {ECO:0000303|PubMed:35398258}; ORFNames=ATEG_03434;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=35398258; DOI=10.1016/j.fgb.2022.103694;
RA   Sun W.W., Li C.Y., Chiang Y.M., Lin T.S., Warren S., Chang F.R.,
RA   Wang C.C.C.;
RT   "Characterization of a silent azaphilone biosynthesis gene cluster in
RT   Aspergillus terreus NIH 2624.";
RL   Fungal Genet. Biol. 160:103694-103694(2022).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       azaterrilone A and other azaphilones, a class of fungal metabolites
CC       characterized by a highly oxygenated pyrano-quinone bicyclic core and
CC       exhibiting a broad range of bioactivities (PubMed:35398258). The first
CC       step of the pathway begins with the non-reducing polyketide synthase
CC       tazA that assembles one acetyl-CoA starter unit, five malonyl-CoA
CC       units, and catalyzes a series of Claisen condensations, methylation,
CC       PT-mediated cyclization, and finally releases the first hexaketide
CC       precursor through the R-domain. The tazA product then undergoes
CC       reduction on its terminal ketone and the following pyran-ring formation
CC       by yet undetermined enzyme(s). Dehydration and enoyl reduction,
CC       possibly involving the trans-enoyl reductase tazE leads to the next
CC       intermediate. TazD is predicted as an acetyltransferase and might
CC       catalyze the acetylation steps leading to the synthesis of azaterrilone
CC       A. Azaterrilone A is not the final product of the taz pathway and both
CC       the highly reducing polyketide synthase tazB and the dual enzyme tazHJ
CC       catalyze late steps of the pathway, leading to the production of the 2
CC       final stereoisomers that contain additional polyketide modification
CC       whose structures have still to be determined (Probable).
CC       {ECO:0000269|PubMed:35398258, ECO:0000305|PubMed:35398258}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:35398258}.
CC   -!- INDUCTION: Expression is positively regulated by the azaterrilone A
CC       cluster-specific transcription factor tazR.
CC       {ECO:0000269|PubMed:35398258}.
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DR   EMBL; CH476597; EAU36708.1; -; Genomic_DNA.
DR   RefSeq; XP_001212612.1; XM_001212612.1.
DR   EnsemblFungi; EAU36708; EAU36708; ATEG_03434.
DR   GeneID; 4318029; -.
DR   VEuPathDB; FungiDB:ATEG_03434; -.
DR   eggNOG; ENOG502SI00; Eukaryota.
DR   HOGENOM; CLU_029138_1_0_1; -.
DR   OMA; TGREHWG; -.
DR   OrthoDB; 542619at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..413
FT                   /note="Azaphilone biosynthesis cluster protein M"
FT                   /id="PRO_0000456076"
SQ   SEQUENCE   413 AA;  46478 MW;  AD99D38F8B255364 CRC64;
     MGSIINIPGL PDGEFSNYEQ VYLKMSRAFS HLQKEHWGIH CVCSVSRDSP NGSTFSETLR
     QAWMRLVVEY PGLSMVPVGL QKKYPRLDEK VVLLIQHWRV DALGTCMLLD RLFEILGQST
     VPSTQPDQVQ PNQPTPSFES AAGASKTEDA DLQAYARDYI DSFHQRAVNA DGLPYRGDAT
     TPPSTTAHWD LDFSVDSTNA IKDACRHHKI SVTAAIHTAL ARTVFSYLTE TECQAGYTTV
     MAVNMRPYLP PPYNSKKHAC QTFVASITPT VPYHSGFVES ARSLTHEYRS WWSADFMRSL
     WWIYEYHLAK LSAPRPANAA PMKPPSGVTL SSLGVVDRNL RGGYGPNLRV DKFRFGVSMM
     TRQTILYAWT FKDRLTLSLN YNDAYYSDLM AREVMSRVAS HLEKGLEVEL NTV