TB10B_MOUSE
ID TB10B_MOUSE Reviewed; 798 AA.
AC Q8BHL3; Q6GQW9; Q6PIZ5; Q91XR3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=TBC1 domain family member 10B;
DE AltName: Full=Protein wz3-85;
GN Name=Tbc1d10b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 180-798.
RC TISSUE=Spleen;
RA Tian W., Chua K., Strober W., Chu C.C.;
RT "Characterization of an unknown wz3-85 gene.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 119-798.
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain cortex;
RX PubMed=17114649; DOI=10.1074/mcp.m600046-mcp200;
RA Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,
RA Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B.,
RA Panse C., Schlapbach R., Mansuy I.M.;
RT "Qualitative and quantitative analyses of protein phosphorylation in naive
RT and stimulated mouse synaptosomal preparations.";
RL Mol. Cell. Proteomics 6:283-293(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-664 AND SER-673, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129; THR-136; SER-647;
RP SER-650 AND SER-673, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [8]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-170, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Acts as GTPase-activating protein for RAB3A, RAB22A, RAB27A,
CC AND RAB35. Does not act on RAB2A and RAB6A (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH25889.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH72576.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAK82984.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC33022.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC33025.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK047320; BAC33022.1; ALT_INIT; mRNA.
DR EMBL; AK047327; BAC33025.1; ALT_INIT; mRNA.
DR EMBL; AC122537; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AF285112; AAK82984.1; ALT_INIT; mRNA.
DR EMBL; BC025889; AAH25889.1; ALT_INIT; mRNA.
DR EMBL; BC072576; AAH72576.2; ALT_INIT; mRNA.
DR CCDS; CCDS40139.2; -.
DR RefSeq; NP_653105.3; NM_144522.5.
DR AlphaFoldDB; Q8BHL3; -.
DR SMR; Q8BHL3; -.
DR BioGRID; 212858; 7.
DR IntAct; Q8BHL3; 2.
DR MINT; Q8BHL3; -.
DR STRING; 10090.ENSMUSP00000113307; -.
DR iPTMnet; Q8BHL3; -.
DR PhosphoSitePlus; Q8BHL3; -.
DR EPD; Q8BHL3; -.
DR jPOST; Q8BHL3; -.
DR MaxQB; Q8BHL3; -.
DR PaxDb; Q8BHL3; -.
DR PeptideAtlas; Q8BHL3; -.
DR PRIDE; Q8BHL3; -.
DR ProteomicsDB; 254855; -.
DR Antibodypedia; 27194; 105 antibodies from 22 providers.
DR DNASU; 68449; -.
DR Ensembl; ENSMUST00000120705; ENSMUSP00000113307; ENSMUSG00000042492.
DR GeneID; 68449; -.
DR KEGG; mmu:68449; -.
DR UCSC; uc009juo.3; mouse.
DR CTD; 26000; -.
DR MGI; MGI:1915699; Tbc1d10b.
DR VEuPathDB; HostDB:ENSMUSG00000042492; -.
DR eggNOG; KOG2221; Eukaryota.
DR GeneTree; ENSGT00940000159805; -.
DR HOGENOM; CLU_005350_8_0_1; -.
DR InParanoid; Q8BHL3; -.
DR OMA; NNWDKWL; -.
DR OrthoDB; 976276at2759; -.
DR PhylomeDB; Q8BHL3; -.
DR TreeFam; TF313293; -.
DR Reactome; R-MMU-8854214; TBC/RABGAPs.
DR BioGRID-ORCS; 68449; 12 hits in 74 CRISPR screens.
DR ChiTaRS; Tbc1d10b; mouse.
DR PRO; PR:Q8BHL3; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8BHL3; protein.
DR Bgee; ENSMUSG00000042492; Expressed in granulocyte and 252 other tissues.
DR ExpressionAtlas; Q8BHL3; baseline and differential.
DR Genevisible; Q8BHL3; MM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005096; F:GTPase activator activity; ISS:UniProtKB.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0043087; P:regulation of GTPase activity; ISS:UniProtKB.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISO:MGI.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; GTPase activation; Methylation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..798
FT /note="TBC1 domain family member 10B"
FT /id="PRO_0000315717"
FT DOMAIN 346..534
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 1..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 618..798
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 702..769
FT /evidence="ECO:0000255"
FT COMPBIAS 11..25
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 618..636
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..651
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 705..790
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4KMP7"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 136
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 170
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 644
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q4KMP7"
FT MOD_RES 647
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 650
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 664
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 673
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT CONFLICT 31
FT /note="G -> R (in Ref. 1; BAC33022/BAC33025)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="P -> S (in Ref. 3; AAK82984)"
FT /evidence="ECO:0000305"
FT CONFLICT 697
FT /note="N -> S (in Ref. 3; AAK82984)"
FT /evidence="ECO:0000305"
FT CONFLICT 751
FT /note="Q -> R (in Ref. 3; AAK82984)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 798 AA; 87275 MW; B9CB616C2BA01913 CRC64;
METGPAPLVA PPRRHGAPAA PSPPPRGSRA GSHLVVEPGP PVTTATSAPV ELVAPGEARP
ACVPGSSQTS ASTPTTATSS TVVMLTLEAS PEAAKTQEFP APAAETGAET SVALALGTDT
QKTEEVRASP VPGPGTPTRT PSRMAPGALT AKPPLAPKPG TTVASGVTAR GGVGQVAGGH
EAATSASAGS VPEDPSGPVT GPPGTCEAPA PTPVAVVTVT PAPEPVENFQ DLGSTSSLGP
GISGPRGQAP DTLSYLDSVS LMSGTLESLP DDVSSMGSDS EINGMALRKT DKYGFLGGSQ
YSGSLESSIP VDVARQRELK WLEMFSNWDK WLSRRFQKVK LRCRKGIPSS LRAKAWQYLS
NSKELLEQNP GKFEELERAA GDPKWLDVIE KDLHRQFPFH EMFAARGGHG QQDLYRILKA
YTIYRPDEGY CQAQAPVAAV LLMHMPAEQA FWCLVQICDK YLPGYYSAGL EAIQLDGEIF
FALLRRVSPL AHRHLRRQRI DPVLYMTEWF MCIFARTLPW ASVLRVWDMF FCEGVKIIFR
VALVLLRHTL GSVEKLRSCQ GMYETMEQLR NLPQQCMQED FLVHEVTNLP VTEAWIEREN
AAQLKKWRET RGELQYRPSR RLHGSRAIHE ERRRQQPPLG PSSSLLSLPS LKSRGSRAVG
GAPSPPPPVR RASAGPVPGA VVIAEGLHPS LPSPTGNSTP LGTSKEIRRQ EKERQKQEKD
REKERQRQEK ERERQEKERQ KWEKEQEKEQ QKQEKERQKL EKKGQGRKLS LRRRADGPPA
SHDGGDRSAA EARQDAYF
