TB22A_MOUSE
ID TB22A_MOUSE Reviewed; 516 AA.
AC Q8R5A6; Q3U268; Q3U3T0; Q8CA49;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 3.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=TBC1 domain family member 22A;
GN Name=Tbc1d22a; Synonyms=D15Ertd781e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Heart, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a GTPase-activating protein for Rab family
CC protein(s). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with ACBD3 and ARFGEF1. Interacts with
CC YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ.
CC {ECO:0000250|UniProtKB:Q8WUA7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8R5A6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R5A6-2; Sequence=VSP_016761;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH23106.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK039627; BAC30403.1; -; mRNA.
DR EMBL; AK154226; BAE32447.1; -; mRNA.
DR EMBL; AK154602; BAE32705.1; -; mRNA.
DR EMBL; AK155459; BAE33274.1; -; mRNA.
DR EMBL; AK155770; BAE33429.1; -; mRNA.
DR EMBL; AK146872; BAE27495.1; -; mRNA.
DR EMBL; BC023106; AAH23106.1; ALT_INIT; mRNA.
DR EMBL; BC066009; AAH66009.1; -; mRNA.
DR CCDS; CCDS27729.1; -. [Q8R5A6-1]
DR RefSeq; NP_663451.2; NM_145476.2. [Q8R5A6-1]
DR RefSeq; XP_017172065.1; XM_017316576.1.
DR AlphaFoldDB; Q8R5A6; -.
DR SMR; Q8R5A6; -.
DR BioGRID; 230190; 4.
DR IntAct; Q8R5A6; 1.
DR MINT; Q8R5A6; -.
DR STRING; 10090.ENSMUSP00000065721; -.
DR iPTMnet; Q8R5A6; -.
DR PhosphoSitePlus; Q8R5A6; -.
DR EPD; Q8R5A6; -.
DR jPOST; Q8R5A6; -.
DR MaxQB; Q8R5A6; -.
DR PaxDb; Q8R5A6; -.
DR PeptideAtlas; Q8R5A6; -.
DR PRIDE; Q8R5A6; -.
DR ProteomicsDB; 263071; -. [Q8R5A6-1]
DR ProteomicsDB; 263072; -. [Q8R5A6-2]
DR Antibodypedia; 242; 164 antibodies from 21 providers.
DR Ensembl; ENSMUST00000063414; ENSMUSP00000065721; ENSMUSG00000051864. [Q8R5A6-1]
DR GeneID; 223754; -.
DR KEGG; mmu:223754; -.
DR UCSC; uc007xea.1; mouse. [Q8R5A6-1]
DR CTD; 25771; -.
DR MGI; MGI:1289265; Tbc1d22a.
DR VEuPathDB; HostDB:ENSMUSG00000051864; -.
DR eggNOG; KOG1092; Eukaryota.
DR GeneTree; ENSGT00940000159840; -.
DR HOGENOM; CLU_018687_6_0_1; -.
DR InParanoid; Q8R5A6; -.
DR OMA; TACWGDE; -.
DR OrthoDB; 750722at2759; -.
DR PhylomeDB; Q8R5A6; -.
DR TreeFam; TF314211; -.
DR BioGRID-ORCS; 223754; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Tbc1d22a; mouse.
DR PRO; PR:Q8R5A6; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8R5A6; protein.
DR Bgee; ENSMUSG00000051864; Expressed in lumbar dorsal root ganglion and 259 other tissues.
DR ExpressionAtlas; Q8R5A6; baseline and differential.
DR Genevisible; Q8R5A6; MM.
DR GO; GO:0071889; F:14-3-3 protein binding; ISS:UniProtKB.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q8WUA7"
FT CHAIN 2..516
FT /note="TBC1 domain family member 22A"
FT /id="PRO_0000208053"
FT DOMAIN 221..445
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 63..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 102..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..132
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..183
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q8WUA7"
FT MOD_RES 144
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT MOD_RES 166
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..41
FT /note="MASDGARKQFWKRSNSKVPGSIQHVYGAQHPPFDPLLHGTL -> MRSGHGQ
FT RRGQEAVLEAQQQQGSRAASSMCMEPSTHRLIRCYMAPC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_016761"
FT CONFLICT 120
FT /note="E -> D (in Ref. 1; BAE33274/BAE32447)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 516 AA; 59362 MW; A838069E77897B10 CRC64;
MASDGARKQF WKRSNSKVPG SIQHVYGAQH PPFDPLLHGT LLKSTPKVPT TPVKAKRVST
FQEFESNTSD AWDAGEDDDE LLAMATESLN SEVVMETAHR VLRNHSQRQS QPSQKTTEPE
PEPQPIAEPP VPPSGDLRLV KSVSESHTPC PSESTGDTVP LQRSQSLPHS ATVTLSGTSD
PHALADSALS KRETSRLDKF KQLLAGPNTD LEELRKLSWS GIPKPVRPMT WKLLSGYLPA
NVDRRPATLQ RKQKEYFAFI EHYYSSRNDE VHQDTYRQIH IDIPRMSPEA LILQPKVTEI
FERILFIWAI RHPASGYVQG INDLVTPFFV VFICEYTDRE DVDKVDVSSV PAEVLRNIEA
DTYWCMSKLL DGIQDNYTFA QPGIQMKVKM LEELVSRIDE RVHRHLDGHE VRYLQFAFRW
MNNLLMRELP LRCTIRLWDT YQSEPEGFSH FHLYVCAAFL VRWRREILEE RDFQELLLFL
QNLPTARWDD QDVSLLLAEA YRLKFAFADA PNHYKK
