ID   TBA4_DROME              Reviewed;         462 AA.
AC   P06606; Q9VT30;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 182.
DE   RecName: Full=Tubulin alpha-4 chain;
GN   Name=alphaTub67C; Synonyms=TubA67C; ORFNames=CG8308;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3095837; DOI=10.1073/pnas.83.22.8477;
RA   Theurkauf W.E., Baum H., Bo J., Wensink P.C.;
RT   "Tissue-specific and constitutive alpha-tubulin genes of Drosophila
RT   melanogaster code for structurally distinct proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:8477-8481(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC   -!- FUNCTION: Tubulin is the major constituent of microtubules. It binds
CC       two moles of GTP, one at an exchangeable site on the beta chain and one
CC       at a non-exchangeable site on the alpha chain.
CC   -!- SUBUNIT: Dimer of alpha and beta chains. A typical microtubule is a
CC       hollow water-filled tube with an outer diameter of 25 nm and an inner
CC       diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to
CC       form protofilaments running lengthwise along the microtubule wall with
CC       the beta-tubulin subunit facing the microtubule plus end conferring a
CC       structural polarity. Microtubules usually have 13 protofilaments but
CC       different protofilament numbers can be found in some organisms and
CC       specialized cells.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- SIMILARITY: Belongs to the tubulin family. {ECO:0000305}.
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DR   EMBL; M14646; AAA28988.1; -; Genomic_DNA.
DR   EMBL; AE014296; AAF50226.2; -; Genomic_DNA.
DR   PIR; D26488; D26488.
DR   RefSeq; NP_524009.2; NM_079285.4.
DR   AlphaFoldDB; P06606; -.
DR   SMR; P06606; -.
DR   BioGRID; 64519; 8.
DR   DIP; DIP-22438N; -.
DR   IntAct; P06606; 1.
DR   STRING; 7227.FBpp0076122; -.
DR   PaxDb; P06606; -.
DR   PRIDE; P06606; -.
DR   EnsemblMetazoa; FBtr0076393; FBpp0076122; FBgn0087040.
DR   GeneID; 39130; -.
DR   KEGG; dme:Dmel_CG8308; -.
DR   CTD; 39130; -.
DR   FlyBase; FBgn0087040; alphaTub67C.
DR   VEuPathDB; VectorBase:FBgn0087040; -.
DR   eggNOG; KOG1376; Eukaryota.
DR   GeneTree; ENSGT00940000164588; -.
DR   HOGENOM; CLU_015718_0_0_1; -.
DR   InParanoid; P06606; -.
DR   OMA; GINYERP; -.
DR   OrthoDB; 514396at2759; -.
DR   PhylomeDB; P06606; -.
DR   BioGRID-ORCS; 39130; 0 hits in 3 CRISPR screens.
DR   GenomeRNAi; 39130; -.
DR   PRO; PR:P06606; -.
DR   Proteomes; UP000000803; Chromosome 3L.
DR   Bgee; FBgn0087040; Expressed in secondary oocyte and 30 other tissues.
DR   ExpressionAtlas; P06606; baseline and differential.
DR   Genevisible; P06606; DM.
DR   GO; GO:0055028; C:cortical microtubule; IDA:FlyBase.
DR   GO; GO:0005737; C:cytoplasm; HDA:FlyBase.
DR   GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; HDA:FlyBase.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR   GO; GO:0005886; C:plasma membrane; HDA:FlyBase.
DR   GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; IBA:GO_Central.
DR   GO; GO:0007417; P:central nervous system development; IMP:FlyBase.
DR   GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:FlyBase.
DR   GO; GO:0040016; P:embryonic cleavage; IMP:FlyBase.
DR   GO; GO:0035038; P:female pronucleus assembly; IMP:FlyBase.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0007017; P:microtubule-based process; IEA:InterPro.
DR   GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR   GO; GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
DR   GO; GO:0051028; P:mRNA transport; IMP:FlyBase.
DR   GO; GO:0000280; P:nuclear division; IMP:FlyBase.
DR   GO; GO:0007422; P:peripheral nervous system development; IMP:FlyBase.
DR   GO; GO:0035046; P:pronuclear migration; IMP:FlyBase.
DR   GO; GO:0035044; P:sperm aster formation; IMP:FlyBase.
DR   Gene3D; 1.10.287.600; -; 1.
DR   Gene3D; 3.30.1330.20; -; 1.
DR   Gene3D; 3.40.50.1440; -; 1.
DR   InterPro; IPR002452; Alpha_tubulin.
DR   InterPro; IPR008280; Tub_FtsZ_C.
DR   InterPro; IPR000217; Tubulin.
DR   InterPro; IPR037103; Tubulin/FtsZ-like_C.
DR   InterPro; IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
DR   InterPro; IPR036525; Tubulin/FtsZ_GTPase_sf.
DR   InterPro; IPR023123; Tubulin_C.
DR   InterPro; IPR017975; Tubulin_CS.
DR   InterPro; IPR003008; Tubulin_FtsZ_GTPase.
DR   PANTHER; PTHR11588; PTHR11588; 1.
DR   Pfam; PF00091; Tubulin; 1.
DR   Pfam; PF03953; Tubulin_C; 1.
DR   PRINTS; PR01162; ALPHATUBULIN.
DR   PRINTS; PR01161; TUBULIN.
DR   SMART; SM00864; Tubulin; 1.
DR   SMART; SM00865; Tubulin_C; 1.
DR   SUPFAM; SSF52490; SSF52490; 1.
DR   SUPFAM; SSF55307; SSF55307; 1.
DR   PROSITE; PS00227; TUBULIN; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Cytoskeleton; GTP-binding; Microtubule; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..462
FT                   /note="Tubulin alpha-4 chain"
FT                   /id="PRO_0000048161"
FT   BINDING         153..159
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   462 AA;  51181 MW;  08B7AEF53E1B7592 CRC64;
     MREVVSIQIG QCGIQIGNAC WELYLLEHGI NLDGSLKTKE ELTASGSSAS VGHDTSANDA
     RTFFTETGNG KQVPRSIFVD LEPTVIDDVR NGCMRELYHP EQLISGKEDA ANNYARGRYS
     IGKEVIDRVT SRLQKIAEQC DSLQGFLIFH SLGGGTGSGF TSLLVERLST DYSKKCKLDF
     AVYPSPKVST AVVEPYNALL TTHSTMDHSD CVFMVDNEAI YDICNNSLGV DRPAYRNLNR
     LIAQIVSSTT ASLRFSGSMN VDLNEFQTNL VPFPRIHFPL VAYAPLMSAE RSAHEQHAIT
     TLTNACFESS NMMVKCDPRA GKFMACCMLY RGDVVPKDVN AAVSAIKSKR HIQFVDWCPT
     GFKIGINYEK PAFVPDGDLA KTSRACCMLS NTTAISVAFS NLSYKFDLMF KKRAFVHWYV
     GEGMEEGEFT EARENIAVLE RDFEEVGLDN AEEGGDEDFD EF