TBC12_DICDI
ID TBC12_DICDI Reviewed; 438 AA.
AC O96904; Q55CU5;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Drainin;
DE AltName: Full=TBC1 domain family member 12 homolog;
GN Name=phgA; ORFNames=DDB_G0269130;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF 275-PHE--LEU-282.
RC STRAIN=AX2;
RX PubMed=10369671; DOI=10.1093/emboj/18.12.3305;
RA Becker M., Matzner M., Gerisch G.;
RT "Drainin required for membrane fusion of the contractile vacuole in
RT Dictyostelium is the prototype of a protein family also represented in
RT man.";
RL EMBO J. 18:3305-3316(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15086784; DOI=10.1111/j.1600-0854.2004.00177.x;
RA Wu W.-I., Yajnik J., Siano M., De Lozanne A.;
RT "Structure-function analysis of the BEACH protein LvsA.";
RL Traffic 5:346-355(2004).
CC -!- FUNCTION: May act as a GTPase-activating protein for Rab family
CC protein(s) (By similarity). Required for osmotic regulation by the
CC contractile vacuole in hypo-osmotic environments. Essential for
CC periodic fusion of the contractile vacuole with the plasma membrane and
CC consequent expulsion of water from the cell body. {ECO:0000250,
CC ECO:0000269|PubMed:10369671, ECO:0000269|PubMed:15086784}.
CC -!- SUBCELLULAR LOCATION: Contractile vacuole membrane. Cytoplasm.
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DR EMBL; U81500; AAD00520.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71915.1; -; Genomic_DNA.
DR RefSeq; XP_646386.1; XM_641294.1.
DR AlphaFoldDB; O96904; -.
DR SMR; O96904; -.
DR IntAct; O96904; 2.
DR MINT; O96904; -.
DR STRING; 44689.DDB0191341; -.
DR PaxDb; O96904; -.
DR PRIDE; O96904; -.
DR EnsemblProtists; EAL71915; EAL71915; DDB_G0269130.
DR GeneID; 8617341; -.
DR KEGG; ddi:DDB_G0269130; -.
DR dictyBase; DDB_G0269130; phgA.
DR eggNOG; KOG2223; Eukaryota.
DR HOGENOM; CLU_015133_3_0_1; -.
DR InParanoid; O96904; -.
DR OMA; EILPNWN; -.
DR PhylomeDB; O96904; -.
DR Reactome; R-DDI-8854214; TBC/RABGAPs.
DR PRO; PR:O96904; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0031267; F:small GTPase binding; IPI:dictyBase.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0033298; P:contractile vacuole organization; IMP:dictyBase.
DR GO; GO:0006971; P:hypotonic response; IMP:dictyBase.
DR GO; GO:0140028; P:pore formation during contractile vacuole discharge; IMP:dictyBase.
DR GO; GO:0051600; P:regulation of endocytosis by exocyst localization; IMP:dictyBase.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; GTPase activation; Membrane; Reference proteome;
KW Vacuole.
FT CHAIN 1..438
FT /note="Drainin"
FT /id="PRO_0000327657"
FT DOMAIN 133..374
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT COILED 54..104
FT /evidence="ECO:0000255"
FT MUTAGEN 275..282
FT /note="Missing: Impairs localization to the contractile
FT vacuole and fusion of the contractile vacuole with the
FT plasma membrane."
FT /evidence="ECO:0000269|PubMed:10369671"
SQ SEQUENCE 438 AA; 50289 MW; B02FA4DA7B9F3B08 CRC64;
MICFDNSTAH TDSNNNNNQC DLPENYKELV TTGKVEKLIL SPSSSLDRPK TLPPKSQEEV
LKHQREYEEI QRKAKKTLER EAKEKEKLDA IRKEKERSLI DARKVWEEDI IPVWEKNSKK
RDIKKIKDLS WRGLPPAVRG KIWRLCIGND LRVTDELFNI FLGHANNAYN KTTSPPLKSS
LSSSSKSPNI NIDLRQANNS IHHSRSPMNT STDGEDDVLD LETTNMALIL QDIQDTFPSL
MIFQKGGPLH SDLIDVLGAY ICYRPDIGYV PGMTFLAAMF LLNMEKCDAF LSLSNHINSV
CFLPFFRQDQ SGIPKYLAAM DSTVEALTPP LHKHFKEIGI SAKNYLVDWI TTLFSKALPL
DVATRIWDLV FIEGEIFIYR TALSILRYFI SDLIQATYDE CIDLFNKLPQ RKISEDKLFE
EIQSIVLDQR KFDKLLEK
