ID   TBC12_MOUSE             Reviewed;         696 AA.
AC   Q6A039; B2RQS2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 2.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=TBC1 domain family member 12;
GN   Name=Tbc1d12; Synonyms=Kiaa0608;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Fetal brain;
RX   PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA   Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA   Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA   Nagase T., Ohara O., Koga H.;
RT   "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT   The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT   identified by screening of terminal sequences of cDNA clones randomly
RT   sampled from size-fractionated libraries.";
RL   DNA Res. 11:205-218(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA   Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA   Thibault P.;
RT   "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL   Immunity 30:143-154(2009).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-205, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen, and
RC   Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=28384198; DOI=10.1371/journal.pone.0174883;
RA   Oguchi M.E., Noguchi K., Fukuda M.;
RT   "TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth
RT   of PC12 cells.";
RL   PLoS ONE 12:E0174883-E0174883(2017).
CC   -!- FUNCTION: RAB11A-binding protein that plays a role in neurite
CC       outgrowth. {ECO:0000250|UniProtKB:M0R7T9}.
CC   -!- SUBUNIT: Interacts with RAB11A; this interaction recruits TBC1D12 to
CC       RAB11A-positive recycling endosomes. {ECO:0000250|UniProtKB:O60347}.
CC   -!- SUBCELLULAR LOCATION: Endosome {ECO:0000269|PubMed:28384198}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD32257.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AK172979; BAD32257.1; ALT_INIT; mRNA.
DR   EMBL; BC138056; AAI38057.1; -; mRNA.
DR   EMBL; BC138057; AAI38058.1; -; mRNA.
DR   RefSeq; NP_666064.3; NM_145952.3.
DR   AlphaFoldDB; Q6A039; -.
DR   SMR; Q6A039; -.
DR   STRING; 10090.ENSMUSP00000037884; -.
DR   iPTMnet; Q6A039; -.
DR   PhosphoSitePlus; Q6A039; -.
DR   jPOST; Q6A039; -.
DR   MaxQB; Q6A039; -.
DR   PaxDb; Q6A039; -.
DR   PRIDE; Q6A039; -.
DR   ProteomicsDB; 263129; -.
DR   DNASU; 209478; -.
DR   GeneID; 209478; -.
DR   KEGG; mmu:209478; -.
DR   CTD; 23232; -.
DR   MGI; MGI:2384803; Tbc1d12.
DR   eggNOG; KOG2223; Eukaryota.
DR   InParanoid; Q6A039; -.
DR   OrthoDB; 798837at2759; -.
DR   PhylomeDB; Q6A039; -.
DR   BioGRID-ORCS; 209478; 2 hits in 74 CRISPR screens.
DR   ChiTaRS; Tbc1d12; mouse.
DR   PRO; PR:Q6A039; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q6A039; protein.
DR   GO; GO:0005776; C:autophagosome; IBA:GO_Central.
DR   GO; GO:0055037; C:recycling endosome; IBA:GO_Central.
DR   GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR   GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR   GO; GO:2000785; P:regulation of autophagosome assembly; IBA:GO_Central.
DR   InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR   InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR   Pfam; PF00566; RabGAP-TBC; 1.
DR   SMART; SM00164; TBC; 1.
DR   SUPFAM; SSF47923; SSF47923; 2.
DR   PROSITE; PS50086; TBC_RABGAP; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Endosome; GTPase activation; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..696
FT                   /note="TBC1 domain family member 12"
FT                   /id="PRO_0000327655"
FT   DOMAIN          405..613
FT                   /note="Rab-GAP TBC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT   REGION          1..59
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          91..121
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          156..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          330..373
FT                   /evidence="ECO:0000255"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:O60347"
FT   MOD_RES         205
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19144319,
FT                   ECO:0007744|PubMed:21183079"
FT   MOD_RES         236
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O60347"
FT   MOD_RES         668
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O60347"
FT   MOD_RES         669
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:O60347"
SQ   SEQUENCE   696 AA;  76810 MW;  5CEFED6E3855E7C3 CRC64;
     MMGPEDAGAC SGRNAELLPV PGPMGQDGKT VPATSGFSGG AVAAEPPEEA GEEEAPPPRQ
     LLQRYLAAAA GPLKPGLGGA EAEEAAAAAV PAARGSGMTN GDSGFLLRQD RRGPEEARRR
     RTCGRPCLLE PADEGVDGAG GLDDWAAPLE DPLRSCCLAA GDTDDPDPAA ATPAGRAVES
     AEPSLGLPDA RFGSRNTFEV SRRQSAGDLL PSAGPSAPLP AAEQGPGGTT ARARRSGGFA
     DFFARNLFPK RTKELKSVVH SAPGWKLFGK VPPRENLQKT SKIIQQEYEA RTGRTCKAAP
     QSSRRKSFAL EPLSTTALIL EDRPPNLPAK SVEEALRHRQ EYDEMVAEAK KREIKEAHKR
     KRIMKERFKQ EESIASAMVI WINEILPNWE VMRSTRRVRE LWWQGLPPSV RGKVWSLAVG
     NELNITPELY EIFLSRAKER WKSFSESSSE NDTEGLSVAD REASLELIKL DISRTFPSLY
     IFQKGGPYHD VLHSILGAYT CYRPDVGYVQ GMSFIAAVLI LNLEEADAFI AFANLLNKPC
     QLAFFRVDHS MMLKYFATFE VFFEENLSKL FLHFKSYNLT PDIYLIDWIF TLYSKSLPLD
     LACRVWDVFC RDGEEFLFRT GLGILRLYED ILLQMDFIHI AQFLTKLPED ITSEKLFSCI
     AAIQMQNSTK KWTQVFASVA KDIKEGDKNT SPALKS