TBC14_RAT
ID TBC14_RAT Reviewed; 694 AA.
AC Q5CD77; Q499U3; Q6I7R4;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=TBC1 domain family member 14;
DE AltName: Full=Spermatogenesis-related factor 2;
DE Short=SRF-2;
DE AltName: Full=Up-regulated in nephrectomized rat kidney #2;
GN Name=Tbc1d14; ORFNames=UR-NR#2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=15758561; DOI=10.1507/endocrj.52.75;
RA Yamano Y., Ohyama K., Ohta M., Sano T., Ritani A., Shimada J., Ashida N.,
RA Yoshida E., Ikehara K., Morishima I.;
RT "A novel spermatogenesis related factor-2 (SRF-2) gene expression affected
RT by TCDD treatment.";
RL Endocr. J. 52:75-81(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 278-694, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=Wistar; TISSUE=Kidney;
RX PubMed=15200410; DOI=10.1111/j.1523-1755.2004.00704.x;
RA Horiba N., Masuda S., Takeuchi A., Saito H., Okuda M., Inui K.;
RT "Gene expression variance based on random sequencing in rat remnant
RT kidney.";
RL Kidney Int. 66:29-45(2004).
CC -!- FUNCTION: Plays a role in the regulation of starvation-induced
CC autophagosome formation. Together with the TRAPPIII complex, regulates
CC a constitutive trafficking step from peripheral recycling endosomes to
CC the early Golgi, maintaining the cycling pool of ATG9 required for
CC initiation of autophagy. {ECO:0000250|UniProtKB:Q9P2M4}.
CC -!- SUBUNIT: Interacts with ULK1. May interact with RAB11A and RAB11B, but
CC does not exhibit any GTPase-activating activity toward these proteins.
CC Interacts with TRAPPC8. {ECO:0000250|UniProtKB:Q9P2M4}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network
CC {ECO:0000250|UniProtKB:Q9P2M4}. Golgi apparatus, trans-Golgi network
CC {ECO:0000250|UniProtKB:Q9P2M4}. Note=After amino acid starvation, Golgi
CC apparatus-associated protein levels increase compared with fed
CC conditions. May be cycling between the Golgi apparatus and an endosomal
CC pool, redistributing to the Golgi apparatus upon starvation.
CC {ECO:0000250|UniProtKB:Q9P2M4}.
CC -!- TISSUE SPECIFICITY: PubMed:15758561 detected expression at the stage of
CC sexual maturation in testis, mainly in the spermatocytes. No expression
CC detected in the ovary, brain, heart, lung, liver and kidney.
CC PubMed:15200410 detected expression in brain, heart, lung, liver,
CC spleen and kidney but not in small intestine.
CC {ECO:0000269|PubMed:15200410, ECO:0000269|PubMed:15758561}.
CC -!- DEVELOPMENTAL STAGE: First detected in the testis at 5 weeks. Level of
CC expression increases up to 7 weeks and maintains even at 63 weeks.
CC {ECO:0000269|PubMed:15758561}.
CC -!- INDUCTION: Up-regulated in nephrectomized kidney.
CC {ECO:0000269|PubMed:15200410}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH99760.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD23893.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD91010.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAD91010.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB117752; BAD91010.1; ALT_SEQ; mRNA.
DR EMBL; BC099760; AAH99760.1; ALT_INIT; mRNA.
DR EMBL; AB108668; BAD23893.1; ALT_INIT; mRNA.
DR RefSeq; NP_001012152.2; NM_001012152.2.
DR RefSeq; NP_001029193.2; NM_001034021.2.
DR RefSeq; NP_001106836.1; NM_001113365.1.
DR AlphaFoldDB; Q5CD77; -.
DR SMR; Q5CD77; -.
DR STRING; 10116.ENSRNOP00000055215; -.
DR PhosphoSitePlus; Q5CD77; -.
DR PaxDb; Q5CD77; -.
DR GeneID; 360956; -.
DR KEGG; rno:360956; -.
DR CTD; 57533; -.
DR RGD; 1309993; Tbc1d14.
DR eggNOG; KOG2223; Eukaryota.
DR InParanoid; Q5CD77; -.
DR OrthoDB; 798837at2759; -.
DR PhylomeDB; Q5CD77; -.
DR Reactome; R-RNO-8854214; TBC/RABGAPs.
DR PRO; PR:Q5CD77; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005776; C:autophagosome; ISO:RGD.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0055037; C:recycling endosome; ISO:RGD.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; ISO:RGD.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0010507; P:negative regulation of autophagy; ISO:RGD.
DR GO; GO:0071955; P:recycling endosome to Golgi transport; ISO:RGD.
DR GO; GO:2000785; P:regulation of autophagosome assembly; ISO:RGD.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 2: Evidence at transcript level;
KW Golgi apparatus; GTPase activation; Phosphoprotein; Reference proteome.
FT CHAIN 1..694
FT /note="TBC1 domain family member 14"
FT /id="PRO_0000319418"
FT DOMAIN 402..612
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 108..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 272..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..292
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 92
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2M4"
FT MOD_RES 296
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2M4"
FT CONFLICT 278..282
FT /note="KKTQK -> GLASM (in Ref. 3; BAD23893)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="N -> S (in Ref. 3; BAD23893)"
FT /evidence="ECO:0000305"
FT CONFLICT 416
FT /note="I -> M (in Ref. 3; BAD23893)"
FT /evidence="ECO:0000305"
FT CONFLICT 420
FT /note="L -> V (in Ref. 3; BAD23893)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="L -> F (in Ref. 3; BAD23893)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 694 AA; 78209 MW; 6DAE2A6B5F47AA1F CRC64;
MTDGNLSTSM NGVALMGILD GRPGDSLQEL QHLSIKAVPR SLSVPDYGPT LKLGALEDRH
SLQSVDSGIP TLEIGNPEPV PCSVVHVKRK QSESEIIPER AFQSACPLPS CTPSAPTGSE
REQAVRKSST FPRTGYDSVK LYSPTSKALS RSDDVSVCSV SSLGTELSTT LSVSNEDILD
LMVTSNSSAI VTLENDDDPQ FTDVTLSSIK ETSDLHQQDC VAETEEGRKL RLLQPFSHFF
TRNLLARKQN ARLDRQRDLG WKLFGKVPLR ETAQKDSKKT QKEYEDKAGR PSRPPSPKQN
VRKNLDFEPL STTALILEDR PANLPAKPAE EAQKHRQQYE EMVVQAKKRE LKEAQRRKKQ
LEERCKVEES IGNAVLTWNN EILPNWETMW CSKKVRDLWW QGIPPSVRGK VWSLAIGNEL
NITHELFDIC LARAKERWRS LSTGGSEVEN EDAGFSAADR EASLELIKLD ISRTFPNLCI
FQQGGPYHDM LHSILGAYTC YRPDVGYVQG MSFIAAVLIL NLDTADAFIA FSNLLNKPCQ
MAFFRVDHGL MLTYFAAFEV FFEENLPKLF AHFKKNNLTA DIYLIDWIFT LYSKSLPLDL
ACRIWDVFCR DGEEFLFRTA LGILKLFEDI LTRMDFIHSA QFLTRLPEDL PADDVFAAIS
TVQMQSRNKK WAQVLSALQK DSREMEEGSP SVRD
