ID   TBC20_BOVIN             Reviewed;         403 AA.
AC   Q2T9Q1;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=TBC1 domain family member 20;
GN   Name=TBC1D20;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: GTPase-activating protein specific for Rab1 and Rab2 small
CC       GTPase families for which it can accelerate the intrinsic GTP
CC       hydrolysis rate by more than five orders of magnitude. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: The arginine and glutamine fingers are critical for the GTPase-
CC       activating mechanism, they pull out Rab's 'switch 2' glutamine and
CC       insert in Rab's active site. {ECO:0000250|UniProtKB:Q96BZ9}.
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DR   EMBL; BC111319; AAI11320.1; -; mRNA.
DR   RefSeq; NP_001033118.1; NM_001038029.1.
DR   AlphaFoldDB; Q2T9Q1; -.
DR   SMR; Q2T9Q1; -.
DR   STRING; 9913.ENSBTAP00000017725; -.
DR   PaxDb; Q2T9Q1; -.
DR   PRIDE; Q2T9Q1; -.
DR   Ensembl; ENSBTAT00000017725; ENSBTAP00000017725; ENSBTAG00000013330.
DR   GeneID; 504543; -.
DR   KEGG; bta:504543; -.
DR   CTD; 128637; -.
DR   VEuPathDB; HostDB:ENSBTAG00000013330; -.
DR   VGNC; VGNC:35634; TBC1D20.
DR   eggNOG; KOG2595; Eukaryota.
DR   GeneTree; ENSGT00390000014944; -.
DR   HOGENOM; CLU_039465_1_0_1; -.
DR   InParanoid; Q2T9Q1; -.
DR   OMA; YPMLCYF; -.
DR   OrthoDB; 1107565at2759; -.
DR   TreeFam; TF105942; -.
DR   Reactome; R-BTA-204005; COPII-mediated vesicle transport.
DR   Proteomes; UP000009136; Chromosome 13.
DR   Bgee; ENSBTAG00000013330; Expressed in thymus and 104 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0030173; C:integral component of Golgi membrane; IEA:Ensembl.
DR   GO; GO:0031965; C:nuclear membrane; IEA:Ensembl.
DR   GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR   GO; GO:0031267; F:small GTPase binding; IEA:Ensembl.
DR   GO; GO:0001675; P:acrosome assembly; IEA:Ensembl.
DR   GO; GO:0090110; P:COPII-coated vesicle cargo loading; IEA:Ensembl.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0007030; P:Golgi organization; IEA:Ensembl.
DR   GO; GO:0070309; P:lens fiber cell morphogenesis; IEA:Ensembl.
DR   GO; GO:0034389; P:lipid droplet organization; IEA:Ensembl.
DR   GO; GO:0044829; P:positive regulation by host of viral genome replication; IEA:Ensembl.
DR   GO; GO:0046726; P:positive regulation by virus of viral protein levels in host cell; IEA:Ensembl.
DR   GO; GO:1902953; P:positive regulation of ER to Golgi vesicle-mediated transport; IEA:Ensembl.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IEA:Ensembl.
DR   GO; GO:0072520; P:seminiferous tubule development; IEA:Ensembl.
DR   GO; GO:0019068; P:virion assembly; IEA:Ensembl.
DR   InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR   InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR   InterPro; IPR045913; TBC20/Gyp8-like.
DR   PANTHER; PTHR20913; PTHR20913; 1.
DR   Pfam; PF00566; RabGAP-TBC; 1.
DR   SMART; SM00164; TBC; 1.
DR   SUPFAM; SSF47923; SSF47923; 2.
DR   PROSITE; PS50086; TBC_RABGAP; 1.
PE   2: Evidence at transcript level;
KW   GTPase activation; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..403
FT                   /note="TBC1 domain family member 20"
FT                   /id="PRO_0000285597"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          60..246
FT                   /note="Rab-GAP TBC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            105
FT                   /note="Arginine finger"
FT                   /evidence="ECO:0000250|UniProtKB:Q96BZ9"
FT   SITE            144
FT                   /note="Glutamine finger"
FT                   /evidence="ECO:0000250|UniProtKB:Q96BZ9"
SQ   SEQUENCE   403 AA;  45913 MW;  B1086CBF43F0FDDC CRC64;
     MALRSARGDG PTSGRWDGGA EKGDFNAKRK KKVAEIYQAL NSEPTDVAAL RRMAISEGGL
     LTDEIRRKVW PKLLNVNTND PPPISGKNLR QMSKDYQQVL LDVRRSLRRF PPGMPEEQRE
     GLQEELIDII LLILERNPQL HYYQGYHDIV VTFLLVLGER LATSLVEKLS THHLRDFMDP
     TMDNTKHILN YLMPIIDQVN PELHDFMQSA EVGTIFALSW LITWFGHVLS DFRHVVRLYD
     FFLACHPLMP IYFAAVIVLY REQEVLDCDC DMASVHHLLS QIPQDLPYET LISRAGDLFV
     QFPPSELARE AAAQPQAEKT AASTFKDFEL ASAQQRPDMV LRQRFRGLLR PDERTKDVLT
     KPRTNRFVKL AVMGLTVALG AAALAVVKSA LEWAPKFQLQ LFP