TBC30_MOUSE
ID TBC30_MOUSE Reviewed; 766 AA.
AC Q69ZT9; B9EHP9; Q6AXF5; Q6PCX1; Q8C8T3;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=TBC1 domain family member 30;
GN Name=Tbc1d30; Synonyms=Kiaa0984;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-642, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: GTPase-activating protein (GAP) with broad specificity. Acts
CC as a GAP for RAB3A. Also exhibits significant GAP activity toward
CC RAB22A, RAB27A, and RAB35 in vitro (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH79587.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD32357.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK173079; BAD32357.1; ALT_INIT; mRNA.
DR EMBL; AK044521; BAC31963.1; -; mRNA.
DR EMBL; BC059081; AAH59081.1; -; mRNA.
DR EMBL; BC079587; AAH79587.1; ALT_FRAME; mRNA.
DR EMBL; BC138285; AAI38286.1; -; mRNA.
DR EMBL; BC138286; AAI38287.1; -; mRNA.
DR CCDS; CCDS48704.1; -.
DR RefSeq; NP_083333.1; NM_029057.1.
DR AlphaFoldDB; Q69ZT9; -.
DR SMR; Q69ZT9; -.
DR IntAct; Q69ZT9; 1.
DR STRING; 10090.ENSMUSP00000070488; -.
DR iPTMnet; Q69ZT9; -.
DR PhosphoSitePlus; Q69ZT9; -.
DR MaxQB; Q69ZT9; -.
DR PaxDb; Q69ZT9; -.
DR PRIDE; Q69ZT9; -.
DR ProteomicsDB; 263132; -.
DR Ensembl; ENSMUST00000064107; ENSMUSP00000070488; ENSMUSG00000052302.
DR GeneID; 74694; -.
DR KEGG; mmu:74694; -.
DR UCSC; uc007hfl.2; mouse.
DR CTD; 23329; -.
DR MGI; MGI:1921944; Tbc1d30.
DR VEuPathDB; HostDB:ENSMUSG00000052302; -.
DR eggNOG; KOG2058; Eukaryota.
DR GeneTree; ENSGT00940000159226; -.
DR HOGENOM; CLU_012937_1_1_1; -.
DR InParanoid; Q69ZT9; -.
DR OMA; KSHLGCG; -.
DR OrthoDB; 1162786at2759; -.
DR PhylomeDB; Q69ZT9; -.
DR TreeFam; TF321898; -.
DR BioGRID-ORCS; 74694; 0 hits in 70 CRISPR screens.
DR ChiTaRS; Tbc1d30; mouse.
DR PRO; PR:Q69ZT9; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q69ZT9; protein.
DR Bgee; ENSMUSG00000052302; Expressed in retrosplenial region and 122 other tissues.
DR ExpressionAtlas; Q69ZT9; baseline and differential.
DR Genevisible; Q69ZT9; MM.
DR GO; GO:0036064; C:ciliary basal body; ISO:MGI.
DR GO; GO:0005929; C:cilium; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005096; F:GTPase activator activity; ISO:MGI.
DR GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:1902018; P:negative regulation of cilium assembly; ISO:MGI.
DR GO; GO:0043547; P:positive regulation of GTPase activity; ISO:MGI.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR InterPro; IPR032738; Tbc1d30_C.
DR Pfam; PF15733; DUF4682; 1.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coiled coil; Membrane; Phosphoprotein; Reference proteome.
FT CHAIN 1..766
FT /note="TBC1 domain family member 30"
FT /id="PRO_0000320653"
FT DOMAIN 87..295
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 380..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 603..647
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 422..491
FT /evidence="ECO:0000255"
FT COMPBIAS 617..640
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 743..766
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 642
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 633
FT /note="S -> G (in Ref. 1; BAD32357 and 2; BAC31963)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 766 AA; 84893 MW; 99E83A2399DCF800 CRC64;
MRQDKLTGSL RRGGRCLKRQ GGGGVGTILS NVLKKRSCIS RTAPRLLCTL EPGVDTKLKF
TLEPSLGQNG FQQWYDALKA VARLSTGIPK EWRRKVWLTL ADHYLHSIAI DWDKTMRFTF
NERSNPDDDS MGIQIVKDLH RTGCSSYCGQ EAEQDRVVLK RVLLAYARWN KNVGYCQGFN
ILAALILEVM EGNEGDALKI MIYLIDKVLP ESYFVNNLRA LSVDMAVFRD LLRLKLPELS
QHLDTLQRTA NKESGGGYEP PLTNVFTMQW FLTLFATCLP NHTVLKIWDS VFFEGSEIIL
RVSLAIWAKL GEQIECCETA DEFYGTMGRL TQEMLEQDLL QSHELMQTVY SMAPFPFPQL
AELREKYTYN ITPFPATIKP TSVSGRHSKA RDSDDENGPD DEDAVASAVG CLGPLSGLLA
PELQKYQKQI KEATEEQTLR SNNIAELSPG AINSCRSEYH AAFNSMMMER MTTDINALKR
QYSRIKKKQQ QQLHQVYIRA DKGPVTSILP SQANSSPVIN HLLLGKKMKI TNRAAKNAVI
HVPGHPGGKI SPVPYEDIKT KLNSPWRTHI RVHKKNMPRT KSHLGCGDTV GLIEEQSEGC
KASSLGAAEE FPSGRTVTAH SEGSSGDGDG GGSTPRTIEG QSPEPVFGDA DVDVAAVQVK
LEALELNQRD AAAETEPKVH FPCQRHASEL ADAPGENQTA IKLLPGSTSK TPIFSPFPSV
KPLRKSATAR NLGLYGPTER TPNVHFPQMS RGFNKSGIGN SSTKKR
