ID   TBCB_DICDI              Reviewed;         270 AA.
AC   Q54Z01;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Tubulin-specific chaperone B;
DE   AltName: Full=Tubulin-folding cofactor B;
GN   Name=tbcb; ORFNames=DDB_G0277983;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Binds to alpha-tubulin folding intermediates after their
CC       interaction with cytosolic chaperonin in the pathway leading from newly
CC       synthesized tubulin to properly folded heterodimer. {ECO:0000250}.
CC   -!- SUBUNIT: Supercomplex made of cofactors A to E. Cofactors A and D
CC       function by capturing and stabilizing tubulin in a quasi-native
CC       conformation. Cofactor E binds to the cofactor D-tubulin complex;
CC       interaction with cofactor C then causes the release of tubulin
CC       polypeptides that are committed to the native state (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TBCB family. {ECO:0000305}.
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DR   EMBL; AAFI02000023; EAL68164.1; -; Genomic_DNA.
DR   RefSeq; XP_642051.1; XM_636959.1.
DR   AlphaFoldDB; Q54Z01; -.
DR   SMR; Q54Z01; -.
DR   STRING; 44689.DDB0266632; -.
DR   PaxDb; Q54Z01; -.
DR   EnsemblProtists; EAL68164; EAL68164; DDB_G0277983.
DR   GeneID; 8621263; -.
DR   KEGG; ddi:DDB_G0277983; -.
DR   dictyBase; DDB_G0277983; tbcB.
DR   eggNOG; KOG3206; Eukaryota.
DR   HOGENOM; CLU_067577_2_0_1; -.
DR   InParanoid; Q54Z01; -.
DR   OMA; IGVKYDE; -.
DR   PhylomeDB; Q54Z01; -.
DR   PRO; PR:Q54Z01; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   CDD; cd01789; Ubl_TBCB; 1.
DR   Gene3D; 2.30.30.190; -; 1.
DR   InterPro; IPR036859; CAP-Gly_dom_sf.
DR   InterPro; IPR000938; CAP-Gly_domain.
DR   InterPro; IPR045172; TBCB_N_Ubl.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   Pfam; PF01302; CAP_GLY; 1.
DR   Pfam; PF14560; Ubiquitin_2; 1.
DR   SMART; SM01052; CAP_GLY; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   SUPFAM; SSF74924; SSF74924; 1.
DR   PROSITE; PS00845; CAP_GLY_1; 1.
DR   PROSITE; PS50245; CAP_GLY_2; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Cytoskeleton; Microtubule; Reference proteome.
FT   CHAIN           1..270
FT                   /note="Tubulin-specific chaperone B"
FT                   /id="PRO_0000330825"
FT   DOMAIN          214..256
FT                   /note="CAP-Gly"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
SQ   SEQUENCE   270 AA;  30454 MW;  7154204334C567B0 CRC64;
     MSSTKSDFEI IREYIIGDGK DISVGKAPEG NVRLEITHSV LTGESLGTNN FKNFSLDLKI
     KDFKEKLYRF VGTEPKYMEL ILRDENKVND ICKIDDDDDK TLGSYEPKDG MNVHIIDKDP
     NNFVSELQDI SKAPKPVISE EDYNKREGTY KKWKEENQLK KENDTTATTV TATTTTTNNA
     TDTEIEIKVG DRCKVISDDP TNYDERLGKV QYVGTVEFSS GVWIGVELDL PLGKNDGSVK
     GKQYFQCSPK YGCFAKPKNV LVGDYPEEEI