TBCD8_MOUSE
ID TBCD8_MOUSE Reviewed; 1134 AA.
AC Q9Z1A9; Q3TRZ8; Q3URH3; Q3UU99; Q6P1G8;
DT 03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=TBC1 domain family member 8;
DE AltName: Full=BUB2-like protein 1;
DE AltName: Full=Vascular Rab-GAP/TBC-containing protein;
GN Name=Tbc1d8; Synonyms=Hblp1, Vrp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Epididymis, Hippocampus, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Fetal brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 201-1134.
RC TISSUE=Hematopoietic;
RA Guimaraes M.J., Bazan J.F.;
RT "The CHESS protein domain: a novel structural unit that is common among
RT networks involved in cell signaling, chromatin metabolism and cell
RT division.";
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a GTPase-activating protein for Rab family
CC protein(s).
CC -!- DOMAIN: The arginine and glutamine fingers are critical for the GTPase-
CC activating mechanism, they pull out Rab's 'switch 2' glutamine and
CC insert in Rab's active site. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD00658.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH05421.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK138630; BAE23728.1; -; mRNA.
DR EMBL; AK141520; BAE24715.1; -; mRNA.
DR EMBL; AK162371; BAE36878.1; -; mRNA.
DR EMBL; CH466536; EDL14558.1; -; Genomic_DNA.
DR EMBL; BC065081; AAH65081.1; -; mRNA.
DR EMBL; BC005421; AAH05421.2; ALT_INIT; mRNA.
DR EMBL; U88873; AAD00658.1; ALT_INIT; mRNA.
DR CCDS; CCDS14905.1; -.
DR RefSeq; NP_061245.3; NM_018775.4.
DR AlphaFoldDB; Q9Z1A9; -.
DR SMR; Q9Z1A9; -.
DR BioGRID; 207685; 2.
DR STRING; 10090.ENSMUSP00000049967; -.
DR iPTMnet; Q9Z1A9; -.
DR PhosphoSitePlus; Q9Z1A9; -.
DR EPD; Q9Z1A9; -.
DR MaxQB; Q9Z1A9; -.
DR PaxDb; Q9Z1A9; -.
DR PRIDE; Q9Z1A9; -.
DR ProteomicsDB; 254827; -.
DR Antibodypedia; 32896; 80 antibodies from 17 providers.
DR DNASU; 54610; -.
DR Ensembl; ENSMUST00000054462; ENSMUSP00000049967; ENSMUSG00000003134.
DR GeneID; 54610; -.
DR KEGG; mmu:54610; -.
DR UCSC; uc007atg.3; mouse.
DR CTD; 11138; -.
DR MGI; MGI:1927225; Tbc1d8.
DR VEuPathDB; HostDB:ENSMUSG00000003134; -.
DR eggNOG; KOG4347; Eukaryota.
DR GeneTree; ENSGT00940000158977; -.
DR InParanoid; Q9Z1A9; -.
DR OMA; EDTTRQN; -.
DR OrthoDB; 450360at2759; -.
DR PhylomeDB; Q9Z1A9; -.
DR TreeFam; TF313145; -.
DR BioGRID-ORCS; 54610; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Tbc1d8; mouse.
DR PRO; PR:Q9Z1A9; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9Z1A9; protein.
DR Bgee; ENSMUSG00000003134; Expressed in caudate-putamen and 261 other tissues.
DR ExpressionAtlas; Q9Z1A9; baseline and differential.
DR Genevisible; Q9Z1A9; MM.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR CDD; cd13349; PH-GRAM1_TBC1D8; 1.
DR CDD; cd13353; PH-GRAM2_TBC1D8; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR InterPro; IPR036009; TBC1D8_PH-GRAM1.
DR InterPro; IPR036016; TBC1D8_PH-GRAM2.
DR Pfam; PF02893; GRAM; 2.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00568; GRAM; 2.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW GTPase activation; Reference proteome; Repeat.
FT CHAIN 1..1134
FT /note="TBC1 domain family member 8"
FT /id="PRO_0000208034"
FT DOMAIN 145..212
FT /note="GRAM 1"
FT DOMAIN 285..353
FT /note="GRAM 2"
FT DOMAIN 504..691
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 433..466
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1034..1070
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 434..448
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 551
FT /note="Arginine finger"
FT /evidence="ECO:0000250"
FT SITE 590
FT /note="Glutamine finger"
FT /evidence="ECO:0000250"
FT CONFLICT 349
FT /note="I -> F (in Ref. 1; BAE36878)"
FT /evidence="ECO:0000305"
FT CONFLICT 705
FT /note="A -> D (in Ref. 1; BAE24715)"
FT /evidence="ECO:0000305"
FT CONFLICT 824
FT /note="K -> N (in Ref. 1; BAE36878)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1134 AA; 130057 MW; 1F946A2523488158 CRC64;
MWLKPEEVLL KNALKLWVTQ KSSCYFVLQR RRGHGEGGGR LTGRLVGALD AVLDSSARVA
PFRILLQVPG SQVYSPIACG ATLEEINRHW DWLEQNLLHT LSVFDNKDDI ASFVKGKVKA
LIAEETSSRL AEQEEEPEKF REALVKFEAR FNFPEAEKLV TYYSCCCWKG RVPRQGWLYL
SINHLCFYSF FLGKELKLVI PWVDIQKLER TSNVFLTDTI RITTQNKERD FSTFLNLDEV
FKIMEQLADV TLRRLLDNEV FDLDPDLQEP SQITKRDLEA RAQNEFFRAF FRLPREEKLH
AVADCSLWTP FSRCHTAGRI FSSDSYICFA SREDGCCNVV LPLREVVSIE KMEDTSLLPN
PIIVSIRSKM AFQFIELKDR ENLVEGLLLR LKQVHANHPV HYETSPSDDD MASPVFYSAS
ICTDKFGDLE MVASQSSEER EEKRPLPHPE PLTAVFQQSG SQSPDSRLSR EQIKISLWND
HFVEYGRTVC MFRTEKIRKL VAMGIPESLR GRLWLLFSDA VTDLASHPGY YGNLVEQSLG
RCCLVTEEIE RDLHRSLPEH PAFQNETGIA ALRRVLTAYA HRNPKIGYCQ SMNILTSVLL
LYAKEEEAFW LLVAVCERML PDYFNHRVIG AQVDQSVFEE LIKEQLPELA EHMSDLSALA
SISLSWFLTL FLSIMPLESA VHVVDCFFYD GIKAIFQLGL AVLEANAEEL CSSKDDGQAL
MVLSRFLDHI KNEDSPGPPI GSHHAFFSDD QEPYPVTDIA DLIRDSYEKF GNQSVEQIEH
LRCKHRIRVL QGHEDTTKQN VLRVVIPEVS ILPEDLEELY DLFKRAHMMS CYWEHHRPMA
LRHDPSRPYA EQYRIDARQF AHLFQLVSPW TCGVHTEILA ERLFRLLDDN MDQLIEFKAF
TSCLDIMYNG EMNEKIKLLY RLHIPPALTE NDRDSQSPLK NPLLSTSRPL VLGKPNGDTI
DYQKQLKQMI KDLAKEKDKM EKELPKMSQR EFIQFCKTLY SMFHEDPEEN DLYQAIATVT
TLLLQIGEVG QRGSSSGSCS QECEEPQASA PPEQDSVFAE AGKSPQAFPE TEGDWTVSLE
HILASLLTEQ SLVNFFEKPL NIKSKLENAK LNQYSLKVLE MSHPPQAELK LNDL
