TBCD9_MOUSE
ID TBCD9_MOUSE Reviewed; 1264 AA.
AC Q3UYK3; Q69ZW8; Q8BIJ5; Q8BVP3; Q9CUB3;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=TBC1 domain family member 9;
GN Name=Tbc1d9; Synonyms=Kiaa0882;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 36-1264 (ISOFORM 1).
RC TISSUE=Fetal brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
CC -!- FUNCTION: May act as a GTPase-activating protein for Rab family
CC protein(s).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3UYK3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UYK3-2; Sequence=VSP_025698;
CC -!- DOMAIN: The arginine and glutamine fingers are critical for the GTPase-
CC activating mechanism, they pull out Rab's 'switch 2' glutamine and
CC insert in Rab's active site. {ECO:0000250}.
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DR EMBL; AK017015; BAB30550.1; -; mRNA.
DR EMBL; AK049049; BAC33525.1; -; mRNA.
DR EMBL; AK077081; BAC36601.1; -; mRNA.
DR EMBL; AK134614; BAE22209.1; -; mRNA.
DR EMBL; AK173050; BAD32328.1; -; mRNA.
DR CCDS; CCDS22448.1; -. [Q3UYK3-2]
DR CCDS; CCDS52607.1; -. [Q3UYK3-1]
DR RefSeq; NP_001104774.1; NM_001111304.1. [Q3UYK3-1]
DR RefSeq; NP_082034.1; NM_027758.4. [Q3UYK3-2]
DR AlphaFoldDB; Q3UYK3; -.
DR SMR; Q3UYK3; -.
DR BioGRID; 214627; 6.
DR STRING; 10090.ENSMUSP00000091093; -.
DR iPTMnet; Q3UYK3; -.
DR PhosphoSitePlus; Q3UYK3; -.
DR MaxQB; Q3UYK3; -.
DR PaxDb; Q3UYK3; -.
DR PeptideAtlas; Q3UYK3; -.
DR PRIDE; Q3UYK3; -.
DR ProteomicsDB; 254664; -. [Q3UYK3-1]
DR ProteomicsDB; 254665; -. [Q3UYK3-2]
DR Antibodypedia; 27252; 88 antibodies from 19 providers.
DR DNASU; 71310; -.
DR Ensembl; ENSMUST00000034145; ENSMUSP00000034145; ENSMUSG00000031709. [Q3UYK3-2]
DR Ensembl; ENSMUST00000093393; ENSMUSP00000091093; ENSMUSG00000031709. [Q3UYK3-1]
DR GeneID; 71310; -.
DR KEGG; mmu:71310; -.
DR UCSC; uc009mju.2; mouse. [Q3UYK3-2]
DR UCSC; uc009mjv.2; mouse. [Q3UYK3-1]
DR CTD; 23158; -.
DR MGI; MGI:1918560; Tbc1d9.
DR VEuPathDB; HostDB:ENSMUSG00000031709; -.
DR eggNOG; KOG4347; Eukaryota.
DR GeneTree; ENSGT00940000157878; -.
DR HOGENOM; CLU_003535_1_1_1; -.
DR InParanoid; Q3UYK3; -.
DR OMA; SNATDRH; -.
DR OrthoDB; 1016712at2759; -.
DR PhylomeDB; Q3UYK3; -.
DR TreeFam; TF313145; -.
DR BioGRID-ORCS; 71310; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Tbc1d9; mouse.
DR PRO; PR:Q3UYK3; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q3UYK3; protein.
DR Bgee; ENSMUSG00000031709; Expressed in choroid plexus epithelium and 197 other tissues.
DR Genevisible; Q3UYK3; MM.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR CDD; cd13351; PH-GRAM1_TCB1D9_TCB1D9B; 1.
DR CDD; cd13354; PH-GRAM2_TCB1D9_TCB1D9B; 1.
DR Gene3D; 2.30.29.30; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR InterPro; IPR036014; TCB1D9/TCB1D9B_PH-GRAM1.
DR InterPro; IPR036017; TCB1D9/TCB1D9B_PH-GRAM2.
DR Pfam; PF02893; GRAM; 2.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00568; GRAM; 2.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; GTPase activation; Reference proteome; Repeat.
FT CHAIN 1..1264
FT /note="TBC1 domain family member 9"
FT /id="PRO_0000288500"
FT DOMAIN 146..213
FT /note="GRAM 1"
FT DOMAIN 293..361
FT /note="GRAM 2"
FT DOMAIN 515..702
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT DOMAIN 886..921
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 410..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1119..1162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1123..1137
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 562
FT /note="Arginine finger"
FT /evidence="ECO:0000250"
FT SITE 601
FT /note="Glutamine finger"
FT /evidence="ECO:0000250"
FT VAR_SEQ 121..353
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025698"
FT CONFLICT 376
FT /note="N -> S (in Ref. 1; BAE22209)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="T -> N (in Ref. 1; BAE22209)"
FT /evidence="ECO:0000305"
FT CONFLICT 544
FT /note="G -> D (in Ref. 2; BAD32328)"
FT /evidence="ECO:0000305"
FT CONFLICT 1261
FT /note="A -> R (in Ref. 1; BAC36601)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1264 AA; 143024 MW; CBCB4203FD6C48D3 CRC64;
MWVNPEEVLL ANALWITERA NPYFILQRRK GHGGDGGGGG GLAGLLVGTL DVVLDSSARV
APYRILYQTP DSLVYWTIAC GGSRKEVTEH WEWLEQNLLQ TLSIFENEND VTTFVRGKIQ
GIIAEYNKIN DVKEDEDTEK FKEAIVKFHR LFGMPEEEKL VNYYSCSYWK GRVPRQGWMY
LSINHLCFSS FLMGREAKLV IRWVDITQLE KNATLLLPDM IKVSTRSSEH FFSVFLNINE
TFKLMEQLAN IAMRQLLDNE GFEQDRSLPK LKKKSPKKVS ALKRDLDARA KSERYRALFR
LPKDEKLDGH TDCTLWTPFN KMHILGQMFV STNYICFTSK EENLCSLIIP LREVTIVEKA
DSCSVLPSPL SISTRNRMTF LFANLKDRDF LVQRISDFLQ QTTSRIYSDK EFSGSCNSSD
DEVYSRPSSL VSSSPQRSTS SDADGERPFN LNGNSVPTAT QTLMTMYRRR SPEEFNPKLA
KEFLKEQAWK IHFAEYGQGI CMYRTEKTRE LVLKGIPESM RGELWLLLSG AINEKATHPG
YYEGLVEKSM GKYNLATEEI ERDLHRSLPE HPAFQNEMGI AALRRVLTAY AFRNPNIGYC
QAMNIVTSVL LLYAKEEEAF WLLVALCERM LPDYYNTRVV GALVDQGVFE ELARDYVPQL
YDCMQDLGVI STISLSWFLT LFLSVMPFES AVVVVDCFFY EGIKVIFQLA LAVLDANVDK
LLNCKDDGEA MTVLGRYLDS VTNKDSTLPP IPHLHSLLSD DVGPYPAVDI FRLIGTSYEK
FGTIRADLIE QMRFKQRLKV IQTLEDTTKR NVVRTIVTET SFTIDELEEL YALFKAEHLT
SCYWGGSSNA LDRHDPSLPY LEQYRIDFEQ FKGMFVLLFP WACGTHSDVL ASRLFQLLDE
NGDSLINFRE FVSGLSAACH GDLTEKLKLL YKMHVLPEPS CDQDEPDSAF EATQYFFEDI
TPECTHVVGL DSRGKQSADD GFVTVSLKQD RGKRANSQEN RNYLKLWTAE NKSKSKTAKD
LPKLNQGQFI ELCKTMYNMF SEDPNEQELY HATAAVTSLL LEIGEVGKFF ITQPAKEDAV
PGPPCGQAIP GMLFPKKGSS QSYVVESTEP LTASLAVDSE EHSLGGQMED IKLEDSSPRD
NGACSSMLIS DDDTKDDSSM SSYSVLSAGS HEEDKLHCED IGEDTVLVRS SQGRATLPRS
SSLDRDWAIT FEQFLASLLT EPALVRYFDK PVCMMARVTS AKNIRMMGKP LTSASDYEIS
ALSG
