TBCEL_MOUSE
ID TBCEL_MOUSE Reviewed; 424 AA.
AC Q8C5W3; B9EID8; Q8C7A5;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Tubulin-specific chaperone cofactor E-like protein;
DE AltName: Full=Leucine-rich repeat-containing protein 35;
GN Name=Tbcel; Synonyms=Lrrc35;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Heart, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-41, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as a regulator of tubulin stability. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C5W3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C5W3-2; Sequence=VSP_019253;
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DR EMBL; AK052261; BAC34901.1; -; mRNA.
DR EMBL; AK077017; BAC36568.1; -; mRNA.
DR EMBL; BC139386; AAI39387.1; -; mRNA.
DR EMBL; BC139387; AAI39388.1; -; mRNA.
DR CCDS; CCDS23088.1; -. [Q8C5W3-1]
DR CCDS; CCDS90542.1; -. [Q8C5W3-1]
DR RefSeq; NP_766626.1; NM_173038.3. [Q8C5W3-1]
DR RefSeq; XP_006510456.1; XM_006510393.2.
DR RefSeq; XP_006510457.1; XM_006510394.3.
DR AlphaFoldDB; Q8C5W3; -.
DR SMR; Q8C5W3; -.
DR STRING; 10090.ENSMUSP00000067882; -.
DR iPTMnet; Q8C5W3; -.
DR PhosphoSitePlus; Q8C5W3; -.
DR EPD; Q8C5W3; -.
DR jPOST; Q8C5W3; -.
DR MaxQB; Q8C5W3; -.
DR PaxDb; Q8C5W3; -.
DR PeptideAtlas; Q8C5W3; -.
DR PRIDE; Q8C5W3; -.
DR ProteomicsDB; 254828; -. [Q8C5W3-1]
DR ProteomicsDB; 254829; -. [Q8C5W3-2]
DR Ensembl; ENSMUST00000066148; ENSMUSP00000067882; ENSMUSG00000037287. [Q8C5W3-1]
DR Ensembl; ENSMUST00000066179; ENSMUSP00000065125; ENSMUSG00000037287. [Q8C5W3-2]
DR Ensembl; ENSMUST00000125995; ENSMUSP00000114721; ENSMUSG00000037287. [Q8C5W3-1]
DR Ensembl; ENSMUST00000128959; ENSMUSP00000121164; ENSMUSG00000037287. [Q8C5W3-1]
DR Ensembl; ENSMUST00000138506; ENSMUSP00000116616; ENSMUSG00000037287. [Q8C5W3-1]
DR GeneID; 272589; -.
DR KEGG; mmu:272589; -.
DR UCSC; uc009paw.1; mouse. [Q8C5W3-1]
DR UCSC; uc009pax.1; mouse. [Q8C5W3-2]
DR CTD; 219899; -.
DR MGI; MGI:1925543; Tbcel.
DR VEuPathDB; HostDB:ENSMUSG00000037287; -.
DR eggNOG; KOG2982; Eukaryota.
DR GeneTree; ENSGT00530000063405; -.
DR HOGENOM; CLU_017716_1_1_1; -.
DR InParanoid; Q8C5W3; -.
DR OMA; TYLDWAC; -.
DR OrthoDB; 1495296at2759; -.
DR PhylomeDB; Q8C5W3; -.
DR TreeFam; TF320819; -.
DR BioGRID-ORCS; 272589; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Tbcel; mouse.
DR PRO; PR:Q8C5W3; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8C5W3; protein.
DR Bgee; ENSMUSG00000037287; Expressed in choroid plexus epithelium and 243 other tissues.
DR ExpressionAtlas; Q8C5W3; baseline and differential.
DR Genevisible; Q8C5W3; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0043014; F:alpha-tubulin binding; IBA:GO_Central.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007023; P:post-chaperonin tubulin folding pathway; IBA:GO_Central.
DR GO; GO:0007021; P:tubulin complex assembly; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR044994; TBCE.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR15140; PTHR15140; 1.
DR Pfam; PF14560; Ubiquitin_2; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton;
KW Leucine-rich repeat; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..424
FT /note="Tubulin-specific chaperone cofactor E-like protein"
FT /id="PRO_0000239669"
FT REPEAT 73..98
FT /note="LRR 1"
FT REPEAT 99..123
FT /note="LRR 2"
FT REPEAT 124..147
FT /note="LRR 3"
FT REPEAT 152..173
FT /note="LRR 4"
FT REPEAT 175..196
FT /note="LRR 5"
FT REPEAT 201..222
FT /note="LRR 6"
FT REPEAT 228..249
FT /note="LRR 7"
FT DOMAIN 262..303
FT /note="LRRCT"
FT DOMAIN 334..424
FT /note="Ubiquitin-like"
FT COILED 349..375
FT /evidence="ECO:0000255"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 41
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1
FT /note="M -> MASEVNASSPALNFSEKKM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019253"
FT CONFLICT 275
FT /note="K -> R (in Ref. 1; BAC34901)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 424 AA; 48032 MW; 3C1AD31DF4C7B27A CRC64;
MDQPSGRSFM QVLCEKYSPE NFPYRRGPGV GVHVPATPQG SPMKDRLNLP SVLVLNSCGI
TCAGDEREIA AFCAHVSELD LSDNKLQDWH EVSKIVSNVP QLEFLNLSSN PLSLSVLERT
CAGSFSGVRK LVLNNSKASW ETVHTILQEL PELEELFLCL NDYETVSCPS VCCHSLKLLH
ITDNNLQDWT EIRKLGVMFP SLDTLVLANN HLNAIEEPAD SLARLFPNLR SISLHKSGLQ
SWEDIDKLNS FPKLEEVRLL GIPLLQPYTT EERRKLVVAR LPSVSKLNGS VVTDGEREDS
ERFFIRYYVD VPQEEVPFRY HELITKYGKL EPLAEVDLRP QSSAKVEVHF NDQVEEMSIR
LDQTVAELKK QLKTLVQLPT SSMLLYYFDH EAPFGPEEMK YSSRALHSFG IRDGDKIFVE
SKTK
