ID   TBCE_DANRE              Reviewed;         521 AA.
AC   Q5U378;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Tubulin-specific chaperone E;
DE   AltName: Full=Tubulin-folding cofactor E;
GN   Name=tbce; ORFNames=zgc:123075;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Tubulin-folding protein; involved in the second step of the
CC       tubulin folding pathway.
CC   -!- SUBUNIT: Supercomplex made of cofactors A to E. Cofactors A and D
CC       function by capturing and stabilizing tubulin in a quasi-native
CC       conformation. Cofactor E binds to the cofactor D-tubulin complex;
CC       interaction with cofactor C then causes the release of tubulin
CC       polypeptides that are committed to the native state.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TBCE family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH85669.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC085669; AAH85669.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001035078.2; NM_001039989.2.
DR   RefSeq; XP_005155982.1; XM_005155925.3.
DR   AlphaFoldDB; Q5U378; -.
DR   SMR; Q5U378; -.
DR   STRING; 7955.ENSDARP00000065584; -.
DR   PaxDb; Q5U378; -.
DR   Ensembl; ENSDART00000160639; ENSDARP00000131097; ENSDARG00000099921.
DR   Ensembl; ENSDART00000171837; ENSDARP00000132970; ENSDARG00000099921.
DR   GeneID; 664760; -.
DR   KEGG; dre:664760; -.
DR   CTD; 6905; -.
DR   ZFIN; ZDB-GENE-051030-120; tbce.
DR   eggNOG; KOG3207; Eukaryota.
DR   GeneTree; ENSGT00530000063405; -.
DR   HOGENOM; CLU_017716_5_0_1; -.
DR   InParanoid; Q5U378; -.
DR   OMA; KKYALDW; -.
DR   OrthoDB; 249920at2759; -.
DR   PhylomeDB; Q5U378; -.
DR   TreeFam; TF313455; -.
DR   PRO; PR:Q5U378; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 11.
DR   Bgee; ENSDARG00000099921; Expressed in spleen and 25 other tissues.
DR   ExpressionAtlas; Q5U378; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0043014; F:alpha-tubulin binding; IBA:GO_Central.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0007023; P:post-chaperonin tubulin folding pathway; ISS:UniProtKB.
DR   GO; GO:0007021; P:tubulin complex assembly; IBA:GO_Central.
DR   CDD; cd17044; Ubl_TBCE; 1.
DR   Gene3D; 2.30.30.190; -; 1.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR036859; CAP-Gly_dom_sf.
DR   InterPro; IPR000938; CAP-Gly_domain.
DR   InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR044994; TBCE.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   InterPro; IPR044079; Ubl_TBCE.
DR   PANTHER; PTHR15140; PTHR15140; 1.
DR   Pfam; PF01302; CAP_GLY; 1.
DR   SMART; SM01052; CAP_GLY; 1.
DR   SMART; SM00369; LRR_TYP; 4.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   SUPFAM; SSF74924; SSF74924; 1.
DR   PROSITE; PS00845; CAP_GLY_1; 1.
DR   PROSITE; PS50245; CAP_GLY_2; 1.
PE   2: Evidence at transcript level;
KW   Chaperone; Cytoplasm; Cytoskeleton; Leucine-rich repeat;
KW   Reference proteome; Repeat.
FT   CHAIN           1..521
FT                   /note="Tubulin-specific chaperone E"
FT                   /id="PRO_0000083542"
FT   DOMAIN          24..68
FT                   /note="CAP-Gly"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT   REPEAT          147..168
FT                   /note="LRR 1"
FT   REPEAT          173..194
FT                   /note="LRR 2"
FT   REPEAT          199..220
FT                   /note="LRR 3"
FT   REPEAT          224..245
FT                   /note="LRR 4"
FT   REPEAT          247..268
FT                   /note="LRR 5"
FT   REPEAT          271..292
FT                   /note="LRR 6"
FT   REPEAT          301..322
FT                   /note="LRR 7"
FT   DOMAIN          335..377
FT                   /note="LRRCT"
SQ   SEQUENCE   521 AA;  59043 MW;  F6533A652A98685E CRC64;
     MMLDEAVGRR VCCDGERGTV RYVGPVPPTA GVWLGVEWDH PERGKHDGSH DGVRYFTCRH
     PTGGSFVRPQ KASFGVDYVT ALKQRYEVEI EEVTAEEMKI SSKTVVMVGF ENVKKKQSVK
     NLTEVGLRRC EVSAPGPENE IRNTTPFVQS LDLSGNLLSS WEVLAAITEQ LDSLQELHLS
     HNRLSISSAP SSLSSAFSHL RVLSINSCAL TWTQVLHCAP MWQQVEELYL ADNNITELLR
     PEHVLQALTV LDLSNNQIAQ ETVLEISHLP RLERLNLSST SLSEIKFSDV PAGKKTTLFP
     ALKELLLDDN NISEWRVVNE LEKLPSLVYL SCRRNPLLHK EKNLETARQI MIARLGQLEL
     LDMRQILSDE RRGAELDYCK MFGSAWLRAG GHREAEKNNP NTDFMTEHPR YLTLIQKYGA
     PDEGELREQK PFALKNQLLT ITFLCPEDLE RKPIEKKLPG SMIVQKVKGL LHRLLKLPGV
     ELKLTYTCAK MADREIEIDN DLKPLQFYSV EDGDKILVRW S