TBCE_DROME
ID TBCE_DROME Reviewed; 523 AA.
AC A1Z6J5; A1Z6J4; Q8MT85;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Tubulin-specific chaperone E {ECO:0000303|PubMed:19297412};
DE AltName: Full=Tubulin-folding cofactor E {ECO:0000305};
GN Name=Tbce {ECO:0000303|PubMed:19297412, ECO:0000312|FlyBase:FBgn0033055};
GN ORFNames=CG7861 {ECO:0000312|FlyBase:FBgn0033055};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM48343.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM48343.1};
RC TISSUE=Ovary {ECO:0000312|EMBL:AAM48343.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ACH92265.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:ACH92265.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19297412; DOI=10.1242/dev.029983;
RA Jin S., Pan L., Liu Z., Wang Q., Xu Z., Zhang Y.Q.;
RT "Drosophila Tubulin-specific chaperone E functions at neuromuscular
RT synapses and is required for microtubule network formation.";
RL Development 136:1571-1581(2009).
CC -!- FUNCTION: Tubulin-folding protein which is required for the development
CC of the neuronal microtubule network. Essential for the development and
CC function of neuromuscular synapses. Likely to promote microtubule
CC formation by acting in the negative regulation of the microtubule-
CC severing protein spas. {ECO:0000269|PubMed:19297412}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19297412}.
CC -!- DEVELOPMENTAL STAGE: In embryos, expressed ubiquitously with higher
CC expression levels in the central nervous system and muscles (at protein
CC level). Expression levels decrease from the embryonic to larval stage
CC (at protein level). In third-stage larva, high levels of expression in
CC the epidermal cells with lower levels of expression in the muscles,
CC central neurons and peripheral axons (at protein level).
CC {ECO:0000269|PubMed:19297412}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethal, with a few escapers that
CC develop to first-instar larvae. In larval muscles the microtubule (MT)
CC network is greatly reduced with a decrease in the number and length of
CC MT fibers. RNAi-mediated knockdown in larval neurons and muscles
CC results in a significant increase in roll-over time. RNAi-mediated
CC knockdown in pre- and post-synaptic neurons results in increased
CC branching number, increased bouton number and decreased bouton size at
CC the neuromuscular junction (NMJ) synaptic terminals. Presynaptic
CC knockdown also results in increased excitatory junction potentials
CC (EJPs) and miniature excitatory junction potentials (mEJPs) of NMJ
CC synapses, whereas knockdown in postsynaptic neurons has no effect on
CC neurotransmission parameters. {ECO:0000269|PubMed:19297412}.
CC -!- SIMILARITY: Belongs to the TBCE family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57288.3; -; Genomic_DNA.
DR EMBL; AE013599; AAS64773.1; -; Genomic_DNA.
DR EMBL; AY118314; AAM48343.1; -; mRNA.
DR EMBL; BT044200; ACH92265.1; -; mRNA.
DR RefSeq; NP_610197.2; NM_136353.2.
DR RefSeq; NP_995747.1; NM_206025.2.
DR AlphaFoldDB; A1Z6J5; -.
DR SMR; A1Z6J5; -.
DR IntAct; A1Z6J5; 1.
DR STRING; 7227.FBpp0085327; -.
DR PaxDb; A1Z6J5; -.
DR PRIDE; A1Z6J5; -.
DR DNASU; 35532; -.
DR EnsemblMetazoa; FBtr0085974; FBpp0085327; FBgn0033055.
DR EnsemblMetazoa; FBtr0335286; FBpp0307274; FBgn0033055.
DR GeneID; 35532; -.
DR KEGG; dme:Dmel_CG7861; -.
DR UCSC; CG7861-RA; d. melanogaster.
DR UCSC; CG7861-RB; d. melanogaster.
DR CTD; 6905; -.
DR FlyBase; FBgn0033055; Tbce.
DR VEuPathDB; VectorBase:FBgn0033055; -.
DR eggNOG; KOG3207; Eukaryota.
DR GeneTree; ENSGT00530000063405; -.
DR HOGENOM; CLU_017716_5_0_1; -.
DR OMA; KKYALDW; -.
DR OrthoDB; 249920at2759; -.
DR PhylomeDB; A1Z6J5; -.
DR BioGRID-ORCS; 35532; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35532; -.
DR PRO; PR:A1Z6J5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033055; Expressed in embryonic/larval hemocyte (Drosophila) and 23 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0043014; F:alpha-tubulin binding; IBA:GO_Central.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0046785; P:microtubule polymerization; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007274; P:neuromuscular synaptic transmission; IMP:FlyBase.
DR GO; GO:0007023; P:post-chaperonin tubulin folding pathway; IBA:GO_Central.
DR GO; GO:0008582; P:regulation of synaptic assembly at neuromuscular junction; IMP:FlyBase.
DR GO; GO:0007021; P:tubulin complex assembly; IBA:GO_Central.
DR CDD; cd17044; Ubl_TBCE; 1.
DR Gene3D; 2.30.30.190; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR036859; CAP-Gly_dom_sf.
DR InterPro; IPR000938; CAP-Gly_domain.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR044994; TBCE.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR044079; Ubl_TBCE.
DR PANTHER; PTHR15140; PTHR15140; 1.
DR Pfam; PF01302; CAP_GLY; 1.
DR SMART; SM01052; CAP_GLY; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR SUPFAM; SSF74924; SSF74924; 1.
DR PROSITE; PS50245; CAP_GLY_2; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; Leucine-rich repeat; Neurogenesis;
KW Reference proteome; Repeat.
FT CHAIN 1..523
FT /note="Tubulin-specific chaperone E"
FT /id="PRO_0000439453"
FT DOMAIN 31..75
FT /note="CAP-Gly"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT REPEAT 155..180
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 181..204
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 209..232
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 235..258
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 260..284
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 285..310
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 315..337
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT CONFLICT 103
FT /note="E -> V (in Ref. 3; AAM48343)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 523 AA; 59424 MW; 814DF349F33D53FD CRC64;
MVGIIDEVQL FYPLGTRIKI GDNYGTVRYV GEVSGHMGSW LGIEWDDGLR GKHNGIVDGK
RYFQTQTPTG GSFIRPGKVG PCATLEDAAR ERYLNYDSSN VDESLIREAQ ASLQASLFEV
VGMDKIARKQ SKFEQLEEVS VDQTPVNAAG YLKELTHLTT LNVSHTLIWN WEIVASIAQQ
LPSLTNLNLS SNRLVLPTSS QITELEPSFR QLKRINLRSC GFSDWKDVMH TALLWPNILS
LGLQENSLGQ LAEVDRTKIF KQLHELDLHR TNIMDFDQVT KLGNLTTLRL LNIMENGIEE
IKLPDCDSQE KLNIFVSLEQ LNLLHNPIWN EADAFNELDK LPQLKRLSKT PHLKSNFDEM
FSKAVASIAS LQFINKAEVT AEQRRGAEYD IWKKYALDWM QATQGGTDSL REFCRRHRTY
PLLVKKYGSP ADFVPRSQAK QSNLINVSIR HQLTGETWEK KVPRMITVQT LQGLVMKRFR
LSGDVPQLCY VDALHPDLVV PLDNNAKTLD FYSVQEHDTV LVQ
