TBCK_MOUSE
ID TBCK_MOUSE Reviewed; 762 AA.
AC Q8BM85; Q8BWD3; Q8BY53;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=TBC domain-containing protein kinase-like protein;
GN Name=Tbck;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAC28746.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAC28746.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:BAC28746.1},
RC Mammary gland {ECO:0000312|EMBL:BAC35172.1}, and
RC Thymus {ECO:0000312|EMBL:BAC31100.1};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in the modulation of mTOR signaling and expression
CC of mTOR complex components. Involved in the regulation of cell
CC proliferation and growth. Involved in the control of actin-cytoskeleton
CC organization. {ECO:0000250|UniProtKB:Q8TEA7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8TEA7}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q8TEA7}.
CC Midbody {ECO:0000250|UniProtKB:Q8TEA7}. Note=Mainly localized in the
CC cytoplasm during interphase. During metaphase, TBCK accumulates at the
CC mitotic spindle. At the end of mitosis, it is detected at the midbody.
CC {ECO:0000250|UniProtKB:Q8TEA7}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:16141072};
CC IsoId=Q8BM85-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:16141072};
CC IsoId=Q8BM85-2; Sequence=VSP_052276, VSP_052277;
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive. {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC31100.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK034533; BAC28746.1; -; mRNA.
DR EMBL; AK041928; BAC31100.1; ALT_INIT; mRNA.
DR EMBL; AK052841; BAC35172.1; -; mRNA.
DR RefSeq; NP_001156927.1; NM_001163455.2.
DR AlphaFoldDB; Q8BM85; -.
DR SMR; Q8BM85; -.
DR BioGRID; 234859; 11.
DR IntAct; Q8BM85; 11.
DR STRING; 10090.ENSMUSP00000129205; -.
DR iPTMnet; Q8BM85; -.
DR PhosphoSitePlus; Q8BM85; -.
DR EPD; Q8BM85; -.
DR MaxQB; Q8BM85; -.
DR PaxDb; Q8BM85; -.
DR PeptideAtlas; Q8BM85; -.
DR PRIDE; Q8BM85; -.
DR ProteomicsDB; 262949; -. [Q8BM85-1]
DR ProteomicsDB; 262950; -. [Q8BM85-2]
DR DNASU; 271981; -.
DR GeneID; 271981; -.
DR KEGG; mmu:271981; -.
DR CTD; 93627; -.
DR MGI; MGI:2445052; Tbck.
DR eggNOG; KOG1093; Eukaryota.
DR InParanoid; Q8BM85; -.
DR OrthoDB; 472287at2759; -.
DR PhylomeDB; Q8BM85; -.
DR BioGRID-ORCS; 271981; 3 hits in 75 CRISPR screens.
DR ChiTaRS; Tbck; mouse.
DR PRO; PR:Q8BM85; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8BM85; protein.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0072686; C:mitotic spindle; ISO:MGI.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0030036; P:actin cytoskeleton organization; ISO:MGI.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0008283; P:cell population proliferation; ISO:MGI.
DR GO; GO:0032006; P:regulation of TOR signaling; ISO:MGI.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..762
FT /note="TBC domain-containing protein kinase-like protein"
FT /id="PRO_0000273279"
FT DOMAIN 1..273
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 466..651
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT VAR_SEQ 687..688
FT /note="EI -> GF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_052276"
FT VAR_SEQ 689..762
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_052277"
SQ SEQUENCE 762 AA; 86371 MW; 200651B920B1F9D4 CRC64;
MFPLKDAEMG AFTFFASALP HDVCGSNGLP LTPNSIKILG RFQILKTITH PRLCQYVDIS
RGKHERLVVV AEHCERSLED LLRERKPVSH STVLCIAYEV LEGLHYLNKH GIVHRALSPH
NILLDRKGHI KLAKFGLYHM TAHGDDVDFP IGYPSYLAPE VIAQGIPKTT DHVPSEKPLP
SGPKSDVWSL GIILFELCVG RKLFQSLDVS ERVKFLLTLG CVDDTIIVLA EEHGCLDIIK
ELPENVINIL KKCLTFHPSK RPTPDELMKD QVFSEVSPLY TPFIKPASLF SSSLRCADLT
LPEDISDLCK DIDNDYLAER SIEEVYYLWC LAGGDLEKEL INKEIIRSKP PVCTLPNFLF
EDGESFGQGR DRSSLLDDTT VTLSLCQLRN RLKDVGGEAF YPLLEDDQSN LPHSNSNNEL
SAAATLPLII RERDTEYQLN RIILFDRLLK AYPYKKNQIW KEARVDIPPL MRGLTWAALL
GVEGAIHAKY DAIDKDTPIP TDRQIEVDIP RCHQYDELLS SPEGHAKFRR VLKAWVVSHP
DLVYWQGLDS LCAPFLYLNF NNEALAYACM SAFIPKYLYN FFLKDNSHVI QEYLTVFSQM
IAFHDPELSN HLNEIGFIPD LYAIPWFLTM FTHVFPLHKI FHLWDTLLLG NSSFPFCIGV
AILQQLRDRL LANGFNECIL LFSDLPEIDI ERCVRESVNL FCWTPKSATY RQHAQPPKPA
SESSVVRSSA PYFSAECTDP PKTDLGNKAS PVWKMHCAAP RA
