TBD2B_MOUSE
ID TBD2B_MOUSE Reviewed; 965 AA.
AC Q3U0J8; Q2VPQ4; Q6PHN8; Q6ZPZ5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=TBC1 domain family member 2B;
GN Name=Tbc1d2b; Synonyms=Kiaa1055;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 447-965.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-959, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: GTPase-activating protein that plays a role in the early
CC steps of endocytosis. {ECO:0000250|UniProtKB:Q9UPU7}.
CC -!- SUBCELLULAR LOCATION: Early endosome {ECO:0000250|UniProtKB:Q9UPU7}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98083.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK129273; BAC98083.1; ALT_INIT; mRNA.
DR EMBL; AK156788; BAE33855.1; -; mRNA.
DR EMBL; BC056467; AAH56467.2; -; mRNA.
DR EMBL; BC108420; AAI08421.1; -; mRNA.
DR CCDS; CCDS23401.1; -.
DR RefSeq; NP_919315.2; NM_194334.2.
DR AlphaFoldDB; Q3U0J8; -.
DR SMR; Q3U0J8; -.
DR BioGRID; 211878; 2.
DR STRING; 10090.ENSMUSP00000045413; -.
DR iPTMnet; Q3U0J8; -.
DR PhosphoSitePlus; Q3U0J8; -.
DR EPD; Q3U0J8; -.
DR jPOST; Q3U0J8; -.
DR MaxQB; Q3U0J8; -.
DR PaxDb; Q3U0J8; -.
DR PeptideAtlas; Q3U0J8; -.
DR PRIDE; Q3U0J8; -.
DR ProteomicsDB; 263081; -.
DR Antibodypedia; 56232; 37 antibodies from 11 providers.
DR DNASU; 67016; -.
DR Ensembl; ENSMUST00000041767; ENSMUSP00000045413; ENSMUSG00000037410.
DR GeneID; 67016; -.
DR KEGG; mmu:67016; -.
DR UCSC; uc009rac.1; mouse.
DR CTD; 23102; -.
DR MGI; MGI:1914266; Tbc1d2b.
DR VEuPathDB; HostDB:ENSMUSG00000037410; -.
DR eggNOG; KOG2058; Eukaryota.
DR GeneTree; ENSGT00940000157737; -.
DR HOGENOM; CLU_011278_0_0_1; -.
DR InParanoid; Q3U0J8; -.
DR OMA; EQAFFKP; -.
DR OrthoDB; 1162786at2759; -.
DR PhylomeDB; Q3U0J8; -.
DR TreeFam; TF317336; -.
DR BioGRID-ORCS; 67016; 1 hit in 70 CRISPR screens.
DR PRO; PR:Q3U0J8; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q3U0J8; protein.
DR Bgee; ENSMUSG00000037410; Expressed in vault of skull and 229 other tissues.
DR ExpressionAtlas; Q3U0J8; baseline and differential.
DR Genevisible; Q3U0J8; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000195; Rab-GTPase-TBC_dom.
DR InterPro; IPR035969; Rab-GTPase_TBC_sf.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00566; RabGAP-TBC; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00164; TBC; 1.
DR SUPFAM; SSF47923; SSF47923; 2.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50086; TBC_RABGAP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..965
FT /note="TBC1 domain family member 2B"
FT /id="PRO_0000315714"
FT DOMAIN 34..139
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 664..858
FT /note="Rab-GAP TBC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00163"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 257..288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 310..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 339..537
FT /evidence="ECO:0000255"
FT COMPBIAS 257..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 311..340
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UPU7"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UPU7"
FT MOD_RES 475
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UPU7"
FT MOD_RES 959
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 719
FT /note="T -> A (in Ref. 2; BAE33855)"
FT /evidence="ECO:0000305"
FT CONFLICT 865
FT /note="F -> L (in Ref. 2; BAE33855)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 965 AA; 109950 MW; 3329196304E487E4 CRC64;
MPGAGDGVEE SCSGGEGAVP GTGSEAGAVA GREPSRLCGY LQKLSGKGPL RGYRSRWFVF
DSRRCYLYYF KSPQDALPLG HLDIADACFS YQGRDEAAEP GADPPTHFQV HSAGAVTVLK
APNRELMTYW LQELQQKRWE YCNSLDMMKW DSRTSPTPGD FPKGLVARDT TDIISQHPNP
SAEKARTVLA VEAAPGELVG DRAAHQPAPG HPNPINFYSL KQWGNELKNS MSSFRPGRGH
SESRRTVFYT NEEWELLDPP PKDLEESLVP EERKKPMPEG SKGVASSGFP FEFGRNPYKG
KRPLKDIIGS YKNRHSSSDP LLEGTATSSG SSGGPTKPVP EMQLQIQSQQ EELEQLKKDL
SSQKELIRLL QQTVRSSQYD KYFTNPQISQ GVPGDTLELL HQKDEQILGL SGQLERFGLE
KESLQQEVRT LKSKVGELNE RLGMLMETIQ AKDEVIIKLS ACEGSVSSPT LGPSSPLAIP
ASKDQLELDR LKDSLQGYKS QNKFLNKEIL ELSALRRNAE RRERDLMAKY SSLEAKLCQV
ESKYLILLQE MKTPVCSEEQ GPARDVIAQL LEDALQVESQ EQPEQAFVKP HLVSEFDIYG
FRTVPDDDEE EKLVAKVRAL DLKTLYLTDN QEVSTGVKWE NYFASTMNRE MACSPELKNL
IRAGIPHEHR SKVWKWCVDR HTRKFKDSME PDYFQTLLQK ALEKQNPASK QIELDLLRTL
PNNKHYSSPT SEGIQKLRSV LLAFSWRNPD IGYCQGLNRL VAVALLYLDQ EDAFWCLVTI
VEVFMPRDYY TKTLLGSQVD QRVFRDLLSE KLPRLHTHFE QYKVDYTLIT FNWFLVVFVD
SVVSDILFKI WDSFLYEGPK VIFRFALALF KYKEEEILKL QDSMSIFKYL RYFTRTILDA
RKLISISFGD LNPFPLRQIR NRRAYHLEKV RLELTELEAI REDFLRERDT SPDKGELVSD
EEEDT
