ID   TBE_HUMAN               Reviewed;         475 AA.
AC   Q9UJT0; Q5H8W8; Q8NEG3;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 163.
DE   RecName: Full=Tubulin epsilon chain;
DE   AltName: Full=Epsilon-tubulin;
GN   Name=TUBE1; Synonyms=TUBE;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX   PubMed=10620804; DOI=10.1038/71350;
RA   Chang P., Stearns T.;
RT   "Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins
RT   reveal new aspects of centrosome structure and function.";
RL   Nat. Cell Biol. 2:30-35(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14574404; DOI=10.1038/nature02055;
RA   Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA   Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA   Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA   Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA   Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA   Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA   Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA   Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA   Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA   French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA   Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA   Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA   Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA   Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA   Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA   Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA   Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA   Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA   Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA   Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA   Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA   Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA   Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA   Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA   West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA   Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA   Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA   Rogers J., Beck S.;
RT   "The DNA sequence and analysis of human chromosome 6.";
RL   Nature 425:805-811(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBUNIT: Found in a complex with TEDC1, TEDC2, TUBE1 and TUBD1.
CC       {ECO:0000250|UniProtKB:Q9D6T1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome {ECO:0000269|PubMed:10620804}. Note=Associated with
CC       pericentriolar material. {ECO:0000269|PubMed:10620804}.
CC   -!- SIMILARITY: Belongs to the tubulin family. {ECO:0000305}.
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DR   EMBL; AF201334; AAF09585.1; -; mRNA.
DR   EMBL; Z99289; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471051; EAW48272.1; -; Genomic_DNA.
DR   EMBL; BC025405; AAH25405.1; -; mRNA.
DR   EMBL; BC031101; AAH31101.1; -; mRNA.
DR   CCDS; CCDS5100.1; -.
DR   RefSeq; NP_057346.1; NM_016262.4.
DR   AlphaFoldDB; Q9UJT0; -.
DR   SMR; Q9UJT0; -.
DR   BioGRID; 119353; 18.
DR   IntAct; Q9UJT0; 5.
DR   STRING; 9606.ENSP00000357651; -.
DR   DrugBank; DB00570; Vinblastine.
DR   GlyConnect; 1863; 7 N-Linked glycans (1 site).
DR   GlyGen; Q9UJT0; 1 site, 6 N-linked glycans (1 site).
DR   iPTMnet; Q9UJT0; -.
DR   PhosphoSitePlus; Q9UJT0; -.
DR   BioMuta; TUBE1; -.
DR   DMDM; 8928405; -.
DR   EPD; Q9UJT0; -.
DR   jPOST; Q9UJT0; -.
DR   MassIVE; Q9UJT0; -.
DR   MaxQB; Q9UJT0; -.
DR   PaxDb; Q9UJT0; -.
DR   PeptideAtlas; Q9UJT0; -.
DR   PRIDE; Q9UJT0; -.
DR   ProteomicsDB; 84643; -.
DR   Antibodypedia; 19362; 196 antibodies from 25 providers.
DR   DNASU; 51175; -.
DR   Ensembl; ENST00000368662.10; ENSP00000357651.5; ENSG00000074935.14.
DR   GeneID; 51175; -.
DR   KEGG; hsa:51175; -.
DR   MANE-Select; ENST00000368662.10; ENSP00000357651.5; NM_016262.5; NP_057346.1.
DR   UCSC; uc003pvq.4; human.
DR   CTD; 51175; -.
DR   DisGeNET; 51175; -.
DR   GeneCards; TUBE1; -.
DR   HGNC; HGNC:20775; TUBE1.
DR   HPA; ENSG00000074935; Low tissue specificity.
DR   MIM; 607345; gene.
DR   neXtProt; NX_Q9UJT0; -.
DR   OpenTargets; ENSG00000074935; -.
DR   PharmGKB; PA134936770; -.
DR   VEuPathDB; HostDB:ENSG00000074935; -.
DR   eggNOG; KOG1375; Eukaryota.
DR   GeneTree; ENSGT00940000155723; -.
DR   HOGENOM; CLU_015718_1_0_1; -.
DR   InParanoid; Q9UJT0; -.
DR   OMA; FPSACDD; -.
DR   OrthoDB; 1166669at2759; -.
DR   PhylomeDB; Q9UJT0; -.
DR   TreeFam; TF330882; -.
DR   PathwayCommons; Q9UJT0; -.
DR   SignaLink; Q9UJT0; -.
DR   SIGNOR; Q9UJT0; -.
DR   BioGRID-ORCS; 51175; 309 hits in 1084 CRISPR screens.
DR   ChiTaRS; TUBE1; human.
DR   GeneWiki; TUBE1; -.
DR   GenomeRNAi; 51175; -.
DR   Pharos; Q9UJT0; Tbio.
DR   PRO; PR:Q9UJT0; -.
DR   Proteomes; UP000005640; Chromosome 6.
DR   RNAct; Q9UJT0; protein.
DR   Bgee; ENSG00000074935; Expressed in right hemisphere of cerebellum and 182 other tissues.
DR   ExpressionAtlas; Q9UJT0; baseline and differential.
DR   Genevisible; Q9UJT0; HS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR   GO; GO:0000242; C:pericentriolar material; TAS:ProtInc.
DR   GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; IBA:GO_Central.
DR   GO; GO:0007098; P:centrosome cycle; TAS:ProtInc.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR   Gene3D; 1.10.287.600; -; 1.
DR   Gene3D; 3.40.50.1440; -; 1.
DR   InterPro; IPR004057; Epsilon_tubulin.
DR   InterPro; IPR008280; Tub_FtsZ_C.
DR   InterPro; IPR000217; Tubulin.
DR   InterPro; IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
DR   InterPro; IPR036525; Tubulin/FtsZ_GTPase_sf.
DR   InterPro; IPR023123; Tubulin_C.
DR   InterPro; IPR017975; Tubulin_CS.
DR   InterPro; IPR003008; Tubulin_FtsZ_GTPase.
DR   PANTHER; PTHR11588; PTHR11588; 1.
DR   PANTHER; PTHR11588:SF317; PTHR11588:SF317; 1.
DR   Pfam; PF00091; Tubulin; 1.
DR   Pfam; PF03953; Tubulin_C; 1.
DR   PRINTS; PR01519; EPSLNTUBULIN.
DR   PRINTS; PR01161; TUBULIN.
DR   SMART; SM00864; Tubulin; 1.
DR   SMART; SM00865; Tubulin_C; 1.
DR   SUPFAM; SSF52490; SSF52490; 1.
DR   SUPFAM; SSF55307; SSF55307; 1.
DR   PROSITE; PS00227; TUBULIN; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; GTP-binding; Microtubule; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..475
FT                   /note="Tubulin epsilon chain"
FT                   /id="PRO_0000048486"
FT   BINDING         148..154
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        91
FT                   /note="D -> G (in Ref. 4; AAH31101)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        469
FT                   /note="P -> R (in Ref. 4; AAH31101)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   475 AA;  52932 MW;  3E8E717CBA6AFC80 CRC64;
     MTQSVVVQVG QCGNQIGCCF WDLALREHAA VNQKGIYDEA ISSFFRNVDT RVVGDGGSIS
     KGKICSLKAR AVLIDMEEGV VNEILQGPLR DVFDTKQLIT DISGSGNNWA VGHKVFGSLY
     QDQILEKFRK SAEHCDCLQC FFIIHSMGGG TGSGLGTFLL KVLEDEFPEV YRFVTSIYPS
     GEDDVITSPY NSILAMKELN EHADCVLPID NQSLFDIISK IDLMVNSGKL GTTVKPKSLV
     TSSSGALKKQ HKKPFDAMNN IVANLLLNLT SSARFEGSLN MDLNEISMNL VPFPQLHYLV
     SSLTPLYTLT DVNIPPRRLD QMFSDAFSKD HQLLRADPKH SLYLACALMV RGNVQISDLR
     RNIERLKPSL QFVSWNQEGW KTSLCSVPPV GHSHSLLALA NNTCVKPTFM ELKERFMRLY
     KKKAHLHHYL QVEGMEESCF TEAVSSLSAL IQEYDQLDAT KNMPVQDLPR LSIAM