ID   TBFA_EPTST              Reviewed;         643 AA.
AC   Q90501;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Thread biopolymer filament subunit alpha;
OS   Eptatretus stoutii (Pacific hagfish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Cyclostomata; Myxini;
OC   Myxiniformes; Myxinidae; Eptatretinae; Eptatretus.
OX   NCBI_TaxID=7765;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Slime gland;
RX   PubMed=7535307; DOI=10.1242/jcs.107.11.3133;
RA   Koch E.A., Spitzer R.H., Pithawalla R.B., Parry D.A.D.;
RT   "An unusual intermediate filament subunit from the cytoskeletal biopolymer
RT   released extracellularly into seawater by the primitive hagfish (Eptatretus
RT   stouti).";
RL   J. Cell Sci. 107:3133-3144(1994).
CC   -!- FUNCTION: Released extracellularly into seawater and provides physical
CC       and biological defense against invasive organism by modulation of the
CC       viscoelastic properties of mucus.
CC   -!- SUBUNIT: Coiled-coil heterodimer of an alpha and a gamma subunit.
CC       Assemble into 10 nm filaments. Forms a massive, conical, intermediate
CC       filament biopolymer of approximately 60 cm.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- SIMILARITY: Belongs to the intermediate filament family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR   EMBL; U11865; AAC59661.1; -; mRNA.
DR   PIR; I50539; I50539.
DR   AlphaFoldDB; Q90501; -.
DR   SMR; Q90501; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005882; C:intermediate filament; IEA:UniProtKB-KW.
DR   InterPro; IPR018039; IF_conserved.
DR   InterPro; IPR039008; IF_rod_dom.
DR   Pfam; PF00038; Filament; 1.
DR   SMART; SM01391; Filament; 1.
DR   PROSITE; PS00226; IF_ROD_1; 1.
DR   PROSITE; PS51842; IF_ROD_2; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Direct protein sequencing; Intermediate filament; Secreted.
FT   CHAIN           1..643
FT                   /note="Thread biopolymer filament subunit alpha"
FT                   /id="PRO_0000063863"
FT   DOMAIN          192..510
FT                   /note="IF rod"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT   REGION          1..191
FT                   /note="Head"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          193..227
FT                   /note="Coil 1A"
FT   REGION          228..240
FT                   /note="Linker 1"
FT   REGION          241..341
FT                   /note="Coil 1B"
FT   REGION          342..362
FT                   /note="Linker 12"
FT   REGION          363..381
FT                   /note="Coil 2A"
FT   REGION          382..389
FT                   /note="Linker 2"
FT   REGION          390..510
FT                   /note="Coil 2B"
FT   REGION          511..643
FT                   /note="Tail"
FT   REGION          622..643
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   643 AA;  66694 MW;  FB146930BAFD819C CRC64;
     MSISQTVSKS YTKSVSRGGQ GVSYSQSSSH KVGGGSVRYG TTYSSGGISR VLGFQGGAGG
     AASAGFGGSV GGSGLSRVLG GSMVSGYRSG MGVGGLSLSG TAGLPVSLRG VGAGKALHAI
     TSAFRTRVGG PGTSVGGYGV NYSFLPSTAG PSFGGPFGGP FGGPFGGPLG PGYIDPATLP
     SPDTVQHTRI REKQDLQTLN TKFANLVDQV RTLEQHNAIL KAQISMITSP SDTPEGPVNT
     AVVASTVTAT YNAQIEDLRT TNTALHSEID HLTTIINDIT TKYEEQVEVT RTLETDWNTN
     KDNIDNTYLT IVDLQTKVQG LDEQINTTKQ IYNARVREVQ AAVTGGPTAA YSIRVDNTHQ
     AIDLTTSLQE MKTHYEVLAT KSREEAFTQV QPRIQEMAVT VQAGPQAIIQ AKEQIHVFKL
     QIDSVHREID RLHRKNTDVE REITVIETNI HTQSDEWTNN INSLKVDLEV IKKQITQYAR
     DYQDLLATKM SLDVEIAAYK KLLDSEETRI SHGGGITITT NAGTFPGGLS AAPGGGASYA
     MVPAGVGGVG LAGVGGYGFR SMGGGGGVGY GAGGGGVGYG VGGGFGGGMG MSMSRMSMGA
     AVGGGSYGSG SGYSGGFGLS SSRAGYSASR KSYSSARSSS RIY