TBG1_DROME
ID TBG1_DROME Reviewed; 475 AA.
AC P23257; Q9VQJ5;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Tubulin gamma-1 chain;
DE AltName: Full=Gamma-1-tubulin;
GN Name=gammaTub23C; Synonyms=Tub23C, TubG, TubG1, TubG23C; ORFNames=CG3157;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1904010; DOI=10.1016/0092-8674(91)90389-g;
RA Zheng Y., Jung M.K., Oakley B.R.;
RT "Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and
RT is associated with the centrosome.";
RL Cell 65:817-823(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-333.
RC STRAIN=Canton-S;
RA Tsoi S.C.M., Huang S.M., Sander M.;
RT "Genomic organization of the Drosophlia 23C genetic interval:
RT identification of 3 genes in the 10Kb region surrounding the RRP1 gene.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INTERACTION WITH OTE.
RX PubMed=22751930; DOI=10.1128/mcb.00814-12;
RA Habermann K., Mirgorodskaya E., Gobom J., Lehmann V., Mueller H.,
RA Bluemlein K., Deery M.J., Czogiel I., Erdmann C., Ralser M.,
RA von Kries J.P., Lange B.M.;
RT "Functional analysis of centrosomal kinase substrates in Drosophila
RT melanogaster reveals a new function of the nuclear envelope component
RT otefin in cell cycle progression.";
RL Mol. Cell. Biol. 32:3554-3569(2012).
CC -!- FUNCTION: Tubulin is the major constituent of microtubules. The gamma
CC chain is found at microtubule organizing centers (MTOC) such as the
CC spindle poles or the centrosome, suggesting that it is involved in the
CC minus-end nucleation of microtubule assembly.
CC -!- SUBUNIT: Interacts with Ote. {ECO:0000269|PubMed:22751930}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the tubulin family. {ECO:0000305}.
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DR EMBL; M61765; AAA28597.1; -; mRNA.
DR EMBL; AE014134; AAF51174.1; -; Genomic_DNA.
DR EMBL; AY069633; AAL39778.1; -; mRNA.
DR EMBL; AF073994; AAC27620.1; -; Genomic_DNA.
DR PIR; B39527; UBFFG.
DR RefSeq; NP_476804.1; NM_057456.4.
DR AlphaFoldDB; P23257; -.
DR SMR; P23257; -.
DR BioGRID; 59721; 28.
DR IntAct; P23257; 4.
DR STRING; 7227.FBpp0077326; -.
DR PaxDb; P23257; -.
DR PRIDE; P23257; -.
DR DNASU; 33501; -.
DR EnsemblMetazoa; FBtr0077641; FBpp0077326; FBgn0260639.
DR GeneID; 33501; -.
DR KEGG; dme:Dmel_CG3157; -.
DR CTD; 33501; -.
DR FlyBase; FBgn0260639; gammaTub23C.
DR VEuPathDB; VectorBase:FBgn0260639; -.
DR eggNOG; KOG1374; Eukaryota.
DR GeneTree; ENSGT00940000156957; -.
DR HOGENOM; CLU_015718_1_0_1; -.
DR InParanoid; P23257; -.
DR OMA; EHGINKE; -.
DR OrthoDB; 687389at2759; -.
DR PhylomeDB; P23257; -.
DR BioGRID-ORCS; 33501; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 33501; -.
DR PRO; PR:P23257; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0260639; Expressed in capitellum (Drosophila) and 25 other tissues.
DR Genevisible; P23257; DM.
DR GO; GO:0005813; C:centrosome; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0000930; C:gamma-tubulin complex; IPI:FlyBase.
DR GO; GO:0000931; C:gamma-tubulin large complex; IDA:FlyBase.
DR GO; GO:0008275; C:gamma-tubulin small complex; IPI:FlyBase.
DR GO; GO:0005874; C:microtubule; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000242; C:pericentriolar material; IDA:FlyBase.
DR GO; GO:0005819; C:spindle; IDA:FlyBase.
DR GO; GO:0005525; F:GTP binding; IDA:FlyBase.
DR GO; GO:0019001; F:guanyl nucleotide binding; IDA:FlyBase.
DR GO; GO:0005200; F:structural constituent of cytoskeleton; IBA:GO_Central.
DR GO; GO:0010457; P:centriole-centriole cohesion; IDA:FlyBase.
DR GO; GO:0007098; P:centrosome cycle; IMP:FlyBase.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR GO; GO:0000212; P:meiotic spindle organization; IBA:GO_Central.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IDA:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; HMP:FlyBase.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0051306; P:mitotic sister chromatid separation; IDA:FlyBase.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR GO; GO:0051726; P:regulation of cell cycle; IMP:FlyBase.
DR CDD; cd02188; gamma_tubulin; 1.
DR Gene3D; 1.10.287.600; -; 1.
DR Gene3D; 3.30.1330.20; -; 1.
DR Gene3D; 3.40.50.1440; -; 1.
DR InterPro; IPR002454; Gamma_tubulin.
DR InterPro; IPR008280; Tub_FtsZ_C.
DR InterPro; IPR000217; Tubulin.
DR InterPro; IPR037103; Tubulin/FtsZ-like_C.
DR InterPro; IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
DR InterPro; IPR036525; Tubulin/FtsZ_GTPase_sf.
DR InterPro; IPR023123; Tubulin_C.
DR InterPro; IPR017975; Tubulin_CS.
DR InterPro; IPR003008; Tubulin_FtsZ_GTPase.
DR PANTHER; PTHR11588; PTHR11588; 1.
DR Pfam; PF00091; Tubulin; 1.
DR Pfam; PF03953; Tubulin_C; 1.
DR PRINTS; PR01164; GAMMATUBULIN.
DR PRINTS; PR01161; TUBULIN.
DR SMART; SM00864; Tubulin; 1.
DR SMART; SM00865; Tubulin_C; 1.
DR SUPFAM; SSF52490; SSF52490; 1.
DR SUPFAM; SSF55307; SSF55307; 1.
DR PROSITE; PS00227; TUBULIN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; GTP-binding; Microtubule; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..475
FT /note="Tubulin gamma-1 chain"
FT /id="PRO_0000048454"
FT REGION 453..475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 142..148
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT CONFLICT 402..403
FT /note="KL -> NV (in Ref. 1; AAA28597)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 475 AA; 53339 MW; AD35304CA1490262 CRC64;
MPSEIITLQL GQCGNQIGFE FWKRLCLEHG ISPSGVLEDF ANDGLDRKDV FFYQADDDHY
IPRAVLLDLE PRVINTIMGS VYSKLYNPEN VYLSKHGGGA GNNWASGYSQ GEKLQEEVFD
IIDREADGSD SLEGFILCHS IAGGTGSGMG SFIMERLADR YPKKLIQTFS VFPNQDEISD
VVVQPYNSML TLKRLTTAAD SVVVLDNTAL NRIACDRLHI QNPSFSQINN LVSTIMSVST
TTLRYPSYMN NNLIGLTAPL IPTPQLHFLM TGYTPLTSDS DIHTQQLVNV RKTTVLDVMR
RLLQPKNMMV STGPDKSNHH CYISILNIIQ GEVDPTQVHK SLQRIRDRKM AQFIPWGPTS
IQVALSRSSP YVQSNHRVSG LMLANHTSIC SLFERALNQY DKLRKRGAFL DQFRREDIFK
DDLNELDESR ETVDCLVQEY EAATREDYMQ FSVKRGNGPV DSKSEDSRSV TSAGS
