ID   TBG1_EUPOC              Reviewed;         461 AA.
AC   P34786;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Tubulin gamma-1 chain;
DE   AltName: Full=Gamma-1-tubulin;
OS   Euplotoides octocarinatus (Freshwater ciliate) (Euplotes octocarinatus).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Hypotrichia; Euplotida; Euplotidae; Euplotes.
OX   NCBI_TaxID=2716877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=3(58)-IX;
RX   PubMed=8294024; DOI=10.1016/0378-1119(93)90487-n;
RA   Liang A., Heckmann K.;
RT   "The macronuclear gamma-tubulin-encoding gene of Euplotes octocarinatus
RT   contains two introns and an in-frame TGA.";
RL   Gene 136:319-322(1993).
RN   [2]
RP   SEQUENCE REVISION.
RA   Liang A.;
RL   Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Tubulin is the major constituent of microtubules. The gamma
CC       chain is found at microtubule organizing centers (MTOC) such as the
CC       spindle poles or the centrosome, suggesting that it is involved in the
CC       minus-end nucleation of microtubule assembly.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the tubulin family. {ECO:0000305}.
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DR   EMBL; X71353; CAA50488.1; -; Genomic_DNA.
DR   EMBL; Y09552; CAA70743.1; -; mRNA.
DR   EMBL; Y09554; CAA70745.1; -; mRNA.
DR   AlphaFoldDB; P34786; -.
DR   SMR; P34786; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000930; C:gamma-tubulin complex; IEA:InterPro.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0007020; P:microtubule nucleation; IEA:InterPro.
DR   GO; GO:0007017; P:microtubule-based process; IEA:InterPro.
DR   CDD; cd02188; gamma_tubulin; 1.
DR   Gene3D; 1.10.287.600; -; 1.
DR   Gene3D; 3.30.1330.20; -; 1.
DR   Gene3D; 3.40.50.1440; -; 1.
DR   InterPro; IPR002454; Gamma_tubulin.
DR   InterPro; IPR008280; Tub_FtsZ_C.
DR   InterPro; IPR000217; Tubulin.
DR   InterPro; IPR037103; Tubulin/FtsZ-like_C.
DR   InterPro; IPR018316; Tubulin/FtsZ_2-layer-sand-dom.
DR   InterPro; IPR036525; Tubulin/FtsZ_GTPase_sf.
DR   InterPro; IPR023123; Tubulin_C.
DR   InterPro; IPR017975; Tubulin_CS.
DR   InterPro; IPR003008; Tubulin_FtsZ_GTPase.
DR   PANTHER; PTHR11588; PTHR11588; 1.
DR   Pfam; PF00091; Tubulin; 1.
DR   Pfam; PF03953; Tubulin_C; 1.
DR   PRINTS; PR01164; GAMMATUBULIN.
DR   PRINTS; PR01161; TUBULIN.
DR   SMART; SM00864; Tubulin; 1.
DR   SMART; SM00865; Tubulin_C; 1.
DR   SUPFAM; SSF52490; SSF52490; 1.
DR   SUPFAM; SSF55307; SSF55307; 1.
DR   PROSITE; PS00227; TUBULIN; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoskeleton; GTP-binding; Microtubule; Nucleotide-binding.
FT   CHAIN           1..461
FT                   /note="Tubulin gamma-1 chain"
FT                   /id="PRO_0000048462"
FT   BINDING         142..148
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   461 AA;  51992 MW;  2E0298598BB99C96 CRC64;
     MPREIITCQV GQCGNQIGME FWKQLCMEHG INPEGILEDF AVNGEDRKDV FFYQADDEHY
     VPRAVLIDLE PRVINGIQKS AYSSLYNPEN IYIAKHGGGA GNNWGRGYTD AEKVQDEILE
     MIDREADGSD SLEGFVLTHS IAGGTGSGFG SYLLERLNDH YPKKLIQTYS VFPIENDVVV
     QPYNCLLSIK RLTLNADCVV VLDNNALTSI AVDRLKILQP TFSQINSIVS TVMAASTTTL
     RYPGYMNNDM VGLIASLVPT PRCHFLMTGY TPLSLDQKFN SVRKTTVLDV MRRLLQTKNI
     MVTGAVKKGA YMSILNVIQG DVDPTQVHKS LQRIRERKLA NFIPWGPASI QVALSKKSPY
     IDSGHKVSGL MLANHTGIRS IFKVLYDQYR TFRKRDAYMN IFKQTKIFED NLDEFDSSDE
     VVKSLIDEYA AAEKMDYINW GNDDDDMQFD PREPPKFSNI Q