ID   TBO2_TIBOB              Reviewed;          95 AA.
AC   P0DQP5;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Toxin Tbo-IT2 {ECO:0000303|PubMed:33406803};
DE   Flags: Precursor;
OS   Tibellus oblongus (Oblong running crab spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Dionycha; Philodromidae; Tibellus.
OX   NCBI_TaxID=336685;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 63-92, FUNCTION,
RP   RECOMBINANT EXPRESSION, TOXIC DOSE, MASS SPECTROMETRY, AMIDATION AT CYS-92,
RP   AND STRUCTURE BY NMR OF 63-92.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=33406803; DOI=10.3390/toxins13010029;
RA   Korolkova Y., Maleeva E., Mikov A., Lobas A., Solovyeva E., Gorshkov M.,
RA   Andreev Y., Peigneur S., Tytgat J., Kornilov F., Lushpa V., Mineev K.,
RA   Kozlov S.;
RT   "New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel
RT   Adaptation of ICK Fold.";
RL   Toxins 13:0-0(2021).
CC   -!- FUNCTION: This recombinant (non-amidated) toxin shows insecticidal
CC       activity on larvae of the housefly Musca domestica and has no activity
CC       on a panel of expressed neuronal receptors and ion channels.
CC       {ECO:0000269|PubMed:33406803}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33406803}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:33406803}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4200.71; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:33406803};
CC   -!- TOXIC DOSE: LD(100) is 200 ug/g when the recombinant non-amidated toxin
CC       is tested on larvae of the housefly Musca domestica.
CC       {ECO:0000269|PubMed:33406803}.
CC   -!- MISCELLANEOUS: Does not show effect on transient receptor potential
CC       cation channels (rTRPV1, mTRPV2, hTRPV3, and rTRPA1). Does not show
CC       effect and insect and arachnid sodium channels (BgNav, DmNav, and
CC       VdNav), as well as on mammals sodium channels (Nav1.4/SCN4A,
CC       Nav1.6/SCN8A, and Nav1.7/SCN9A). Does not show effect on insect and
CC       mammal potassium channels (Shaker, Kv1.1/KCNA1, Kv1.2/KCNA2,
CC       Kv1.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5, Kv1.6/KCNA8, Kv2.1/KCNB1,
CC       Kv3.1/KCNC1, Kv4.3/KCND3, and Kv10.1/KCNH1), and on calcium channels
CC       Cav3.1/CACNA1G. {ECO:0000269|PubMed:33406803}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 02 (plectoxin) family. 02
CC       (plectoxin) subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PDB; 7AY8; NMR; -; A=54-92.
DR   PDBsum; 7AY8; -.
DR   AlphaFoldDB; P0DQP5; -.
DR   SMR; P0DQP5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   Gene3D; 4.10.760.10; -; 1.
DR   InterPro; IPR036836; Agouti_dom_sf.
PE   1: Evidence at protein level;
KW   3D-structure; Amidation; Direct protein sequencing; Disulfide bond;
KW   Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..53
FT                   /evidence="ECO:0000305|PubMed:33406803"
FT                   /id="PRO_0000452714"
FT   CHAIN           54..92
FT                   /note="Toxin Tbo-IT2"
FT                   /evidence="ECO:0000269|PubMed:33406803"
FT                   /id="PRO_0000452715"
FT   MOD_RES         92
FT                   /note="Cysteine amide"
FT                   /evidence="ECO:0000269|PubMed:33406803"
FT   DISULFID        54..68
FT                   /evidence="ECO:0000269|PubMed:33406803,
FT                   ECO:0007744|PDB:7AY8"
FT   DISULFID        61..73
FT                   /evidence="ECO:0000269|PubMed:33406803,
FT                   ECO:0007744|PDB:7AY8"
FT   DISULFID        67..84
FT                   /evidence="ECO:0000269|PubMed:33406803,
FT                   ECO:0007744|PDB:7AY8"
FT   DISULFID        70..92
FT                   /evidence="ECO:0000269|PubMed:33406803,
FT                   ECO:0007744|PDB:7AY8"
FT   DISULFID        75..82
FT                   /evidence="ECO:0000269|PubMed:33406803,
FT                   ECO:0007744|PDB:7AY8"
SQ   SEQUENCE   95 AA;  10413 MW;  630C880444B6F500 CRC64;
     MTMKTLCLSL IVIGVLILVA VKAEDYVNIN SLEEAPEENV NINNLEETPE ESRCIQRHRS
     CRKSSECCGC SVCQCNLFGQ NCQCKSGGLI ACGKK