TBP2_ARATH
ID TBP2_ARATH Reviewed; 200 AA.
AC P28148; Q53YT7;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=TATA-box-binding protein 2 {ECO:0000303|Ref.2};
DE AltName: Full=TATA sequence-binding protein 2 {ECO:0000303|Ref.2};
DE Short=AtTBP2 {ECO:0000303|PubMed:10634912};
DE Short=TBP-2 {ECO:0000303|Ref.2};
DE AltName: Full=TATA-binding factor 2 {ECO:0000303|Ref.2};
DE AltName: Full=TATA-box factor 2 {ECO:0000303|Ref.2};
DE AltName: Full=Transcription initiation factor TFIID TBP-2 subunit {ECO:0000303|PubMed:2197561};
GN Name=TBP2 {ECO:0000303|Ref.2};
GN OrderedLocusNames=At1g55520 {ECO:0000312|Araport:AT1G55520};
GN ORFNames=T5A14.8 {ECO:0000312|EMBL:AAD10645.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2197561; DOI=10.1038/346390a0;
RA Gasch A., Hoffmann A., Horikoshi M., Roeder R.G., Chua N.-H.;
RT "Arabidopsis thaliana contains two genes for TFIID.";
RL Nature 346:390-394(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Lawit S.J., Gurley W.B.;
RT "Binary protein-protein interactions of the Arabidopsis thaliana general
RT transcription factor IId.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION, INTERACTION WITH TFIIB1, AND MUTAGENESIS OF GLU-144; GLU-146 AND
RP LYS-197.
RX PubMed=10634912; DOI=10.2307/3871034;
RA Pan S., Czarnecka-Verner E., Gurley W.B.;
RT "Role of the TATA binding protein-transcription factor IIB interaction in
RT supporting basal and activated transcription in plant cells.";
RL Plant Cell 12:125-136(2000).
RN [9]
RP INTERACTION WITH TAF1.
RX PubMed=17340043; DOI=10.1007/s11103-007-9135-1;
RA Lawit S.J., O'Grady K., Gurley W.B., Czarnecka-Verner E.;
RT "Yeast two-hybrid map of Arabidopsis TFIID.";
RL Plant Mol. Biol. 64:73-87(2007).
RN [10]
RP FUNCTION.
RX PubMed=18668124; DOI=10.1038/emboj.2008.151;
RA Imamura S., Hanaoka M., Tanaka K.;
RT "The plant-specific TFIIB-related protein, pBrp, is a general transcription
RT factor for RNA polymerase I.";
RL EMBO J. 27:2317-2327(2008).
RN [11]
RP SUBUNIT.
RC STRAIN=cv. Wassilewskija;
RX PubMed=21266657; DOI=10.1105/tpc.110.080150;
RA Ding Y., Avramova Z., Fromm M.;
RT "Two distinct roles of ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1) at
RT promoters and within transcribed regions of ATX1-regulated genes.";
RL Plant Cell 23:350-363(2011).
RN [12]
RP INTERACTION WITH PTF2.
RX PubMed=23713077; DOI=10.1093/mp/sst083;
RA Niu Q.K., Liang Y., Zhou J.J., Dou X.Y., Gao S.C., Chen L.Q., Zhang X.Q.,
RA Ye D.;
RT "Pollen-expressed transcription factor 2 encodes a novel plant-specific
RT TFIIB-related protein that is required for pollen germination and
RT embryogenesis in Arabidopsis.";
RL Mol. Plant 6:1091-1108(2013).
RN [13]
RP INTERACTION WITH HAT5/ATHB-1 AND ATHB-7.
RX PubMed=24531799; DOI=10.1007/s00299-014-1576-9;
RA Capella M., Re D.A., Arce A.L., Chan R.L.;
RT "Plant homeodomain-leucine zipper I transcription factors exhibit different
RT functional AHA motifs that selectively interact with TBP or/and TFIIB.";
RL Plant Cell Rep. 33:955-967(2014).
CC -!- FUNCTION: General transcription factor (GTF) that functions at the core
CC of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the
CC TATA box is the initial transcriptional step of the pre-initiation
CC complex (PIC), playing a role in the activation of eukaryotic genes
CC transcribed by RNA polymerase II (By similarity). Interacts with TFIIB1
CC and is required for activated transcription and possibly basal
CC transcription (PubMed:10634912). May act as GTF of RNA polymerase I-
CC dependent transcription and rRNA synthesis. Forms a ternary complex
CC with PBRP1 and the rDNA promoter region (PubMed:18668124).
CC {ECO:0000250|UniProtKB:P20226, ECO:0000269|PubMed:10634912,
CC ECO:0000269|PubMed:18668124}.
CC -!- SUBUNIT: Belongs to the TFIID complex together with the TBP-associated
CC factors (TAFs). Binds DNA as monomer. Interacts with TAF1 (via N-
CC terminus) (PubMed:17340043). Interacts with TFIIB1 (PubMed:10634912).
CC Interacts with PTF2 (PubMed:23713077). Interacts with HAT5/ATHB-1 and
CC ATHB-7 (PubMed:24531799). Component of a nuclear protein complex
CC containing at least TATA binding proteins (TBPs, e.g. TBP1 and TBP2)
CC and ATX1 (PubMed:21266657). {ECO:0000269|PubMed:10634912,
CC ECO:0000269|PubMed:17340043, ECO:0000269|PubMed:21266657,
CC ECO:0000269|PubMed:23713077, ECO:0000269|PubMed:24531799}.
CC -!- INTERACTION:
CC P28148; P41151: HSFA1A; NbExp=2; IntAct=EBI-1247465, EBI-1544927;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P28147}.
CC -!- SIMILARITY: Belongs to the TBP family. {ECO:0000305}.
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DR EMBL; X54995; CAA38742.1; -; mRNA.
DR EMBL; AY463626; AAR28028.1; -; mRNA.
DR EMBL; AC005223; AAD10645.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33257.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33258.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58687.1; -; Genomic_DNA.
DR EMBL; BT006488; AAP21296.1; -; mRNA.
DR EMBL; AK227470; BAE99472.1; -; mRNA.
DR EMBL; AB493506; BAH30344.1; -; mRNA.
DR PIR; S10945; S10945.
DR RefSeq; NP_001321103.1; NM_001333717.1.
DR RefSeq; NP_175948.1; NM_104427.4.
DR RefSeq; NP_849812.1; NM_179481.2.
DR AlphaFoldDB; P28148; -.
DR SMR; P28148; -.
DR BioGRID; 27224; 14.
DR IntAct; P28148; 10.
DR STRING; 3702.AT1G55520.2; -.
DR PaxDb; P28148; -.
DR PRIDE; P28148; -.
DR ProteomicsDB; 234158; -.
DR EnsemblPlants; AT1G55520.1; AT1G55520.1; AT1G55520.
DR EnsemblPlants; AT1G55520.2; AT1G55520.2; AT1G55520.
DR EnsemblPlants; AT1G55520.4; AT1G55520.4; AT1G55520.
DR GeneID; 841999; -.
DR Gramene; AT1G55520.1; AT1G55520.1; AT1G55520.
DR Gramene; AT1G55520.2; AT1G55520.2; AT1G55520.
DR Gramene; AT1G55520.4; AT1G55520.4; AT1G55520.
DR KEGG; ath:AT1G55520; -.
DR Araport; AT1G55520; -.
DR TAIR; locus:2193904; AT1G55520.
DR eggNOG; KOG3302; Eukaryota.
DR HOGENOM; CLU_060161_4_2_1; -.
DR InParanoid; P28148; -.
DR OMA; NCEYEPE; -.
DR OrthoDB; 1219067at2759; -.
DR PhylomeDB; P28148; -.
DR PRO; PR:P28148; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P28148; baseline and differential.
DR Genevisible; P28148; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0140223; F:general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0017025; F:TBP-class protein binding; ISS:TAIR.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IDA:TAIR.
DR CDD; cd04516; TBP_eukaryotes; 1.
DR Gene3D; 3.30.310.10; -; 2.
DR HAMAP; MF_00408; TATA_bind_prot_arch; 1.
DR InterPro; IPR000814; TBP.
DR InterPro; IPR030491; TBP_CS.
DR InterPro; IPR012295; TBP_dom_sf.
DR InterPro; IPR033710; TBP_eukaryotic.
DR PANTHER; PTHR10126; PTHR10126; 1.
DR Pfam; PF00352; TBP; 2.
DR PRINTS; PR00686; TIFACTORIID.
DR PROSITE; PS00351; TFIID; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..200
FT /note="TATA-box-binding protein 2"
FT /id="PRO_0000153977"
FT REPEAT 25..101
FT /note="1"
FT /evidence="ECO:0000255"
FT REPEAT 115..192
FT /note="2"
FT /evidence="ECO:0000255"
FT MUTAGEN 144
FT /note="E->R: Unable to bind to TFIIB1."
FT /evidence="ECO:0000269|PubMed:10634912"
FT MUTAGEN 146
FT /note="E->R: Unable to bind to TFIIB1."
FT /evidence="ECO:0000269|PubMed:10634912"
FT MUTAGEN 197
FT /note="K->E: No effect on the binding to TFIIB1."
FT /evidence="ECO:0000269|PubMed:10634912"
SQ SEQUENCE 200 AA; 22396 MW; CFB9596EF8D9CEDD CRC64;
MADQGTEGSQ PVDLTKHPSG IVPTLQNIVS TVNLDCKLDL KAIALQARNA EYNPKRFAAV
IMRIREPKTT ALIFASGKMV CTGAKSEHLS KLAARKYARI VQKLGFPAKF KDFKIQNIVG
SCDVKFPIRL EGLAYSHSAF SSYEPELFPG LIYRMKLPKI VLLIFVSGKI VITGAKMREE
TYTAFENIYP VLREFRKVQQ
