ID   TBP2_ENTHI              Reviewed;         212 AA.
AC   C4M7H7;
DT   18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=TATA-box-binding protein 2;
DE            Short=EhTBP2 {ECO:0000303|PubMed:20026212};
DE   AltName: Full=TATA sequence-binding protein;
DE   AltName: Full=TATA-binding factor;
DE   AltName: Full=TATA-box factor;
DE   AltName: Full=Transcription initiation factor TFIID TBP subunit;
GN   ORFNames=EHI_112050;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1] {ECO:0000312|EMBL:EAL47145.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RX   PubMed=15729342; DOI=10.1038/nature03291;
RA   Loftus B.J., Anderson I., Davies R., Alsmark U.C., Samuelson J., Amedeo P.,
RA   Roncaglia P., Berriman M., Hirt R.P., Mann B.J., Nozaki T., Suh B., Pop M.,
RA   Duchene M., Ackers J., Tannich E., Leippe M., Hofer M., Bruchhaus I.,
RA   Willhoeft U., Bhattacharya A., Chillingworth T., Churcher C.M., Hance Z.,
RA   Harris B., Harris D., Jagels K., Moule S., Mungall K.L., Ormond D.,
RA   Squares R., Whitehead S., Quail M.A., Rabbinowitsch E., Norbertczak H.,
RA   Price C., Wang Z., Guillen N., Gilchrist C., Stroup S.E., Bhattacharya S.,
RA   Lohia A., Foster P.G., Sicheritz-Ponten T., Weber C., Singh U.,
RA   Mukherjee C., El-Sayed N.M.A., Petri W.A., Clark C.G., Embley T.M.,
RA   Barrell B.G., Fraser C.M., Hall N.;
RT   "The genome of the protist parasite Entamoeba histolytica.";
RL   Nature 433:865-868(2005).
RN   [2] {ECO:0000305, ECO:0000312|EMBL:EAL47145.1}
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RA   Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000305}
RP   IDENTIFICATION.
RX   PubMed=20026212; DOI=10.1016/j.pep.2009.12.007;
RA   Castanon-Sanchez C.A., Luna-Arias J.P., de Dios-Bravo M.G.,
RA   Herrera-Aguirre M.E., Olivares-Trejo J.J., Orozco E., Hernandez J.M.;
RT   "Entamoeba histolytica: a unicellular organism containing two active genes
RT   encoding for members of the TBP family.";
RL   Protein Expr. Purif. 70:48-59(2010).
CC   -!- FUNCTION: General transcription factor that functions at the core of
CC       the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA
CC       box is the initial transcriptional step of the pre-initiation complex
CC       (PIC), playing a role in the activation of eukaryotic genes transcribed
CC       by RNA polymerase II. {ECO:0000305}.
CC   -!- SUBUNIT: Belongs to the TFIID complex together with the TBP-associated
CC       factors (TAFs). Binds DNA as monomer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TBP family. {ECO:0000255}.
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DR   EMBL; DS571329; EAL47145.1; -; Genomic_DNA.
DR   RefSeq; XP_652533.1; XM_647441.1.
DR   AlphaFoldDB; C4M7H7; -.
DR   SMR; C4M7H7; -.
DR   GeneID; 3406838; -.
DR   KEGG; ehi:EHI_112050; -.
DR   VEuPathDB; AmoebaDB:EHI5A_043810; -.
DR   VEuPathDB; AmoebaDB:EHI7A_023660; -.
DR   VEuPathDB; AmoebaDB:EHI8A_114190; -.
DR   VEuPathDB; AmoebaDB:EHI_112050; -.
DR   VEuPathDB; AmoebaDB:KM1_049680; -.
DR   eggNOG; KOG3302; Eukaryota.
DR   InParanoid; C4M7H7; -.
DR   Proteomes; UP000001926; Partially assembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0140223; F:general transcription initiation factor activity; IBA:GO_Central.
DR   GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISS:UniProtKB.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR   CDD; cd04516; TBP_eukaryotes; 1.
DR   Gene3D; 3.30.310.10; -; 2.
DR   HAMAP; MF_00408; TATA_bind_prot_arch; 1.
DR   InterPro; IPR000814; TBP.
DR   InterPro; IPR030491; TBP_CS.
DR   InterPro; IPR012295; TBP_dom_sf.
DR   InterPro; IPR033710; TBP_eukaryotic.
DR   PANTHER; PTHR10126; PTHR10126; 1.
DR   Pfam; PF00352; TBP; 2.
DR   PRINTS; PR00686; TIFACTORIID.
DR   PROSITE; PS00351; TFIID; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..212
FT                   /note="TATA-box-binding protein 2"
FT                   /id="PRO_0000394122"
FT   REPEAT          30..114
FT                   /note="1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          120..201
FT                   /note="2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   212 AA;  23733 MW;  37CCEB5A6D511A66 CRC64;
     MSQLGNICHA DYMSTSTESQ ERSLNNPNDT HPEIVNVVST FQLGVKLELR KIVQKARNAE
     YNPKRFAGAI MRISSPKSTA LIFQTGKIVC TGTRSIEESK IASKKYAKII KKIGYPIHYS
     NFNVQNIVGS CDVKFQIALR TLVDSYLAFC QYEPEVFPGL VYRMASPKVT LLVFSTGKVV
     LTGAKDEESL NLAYKNIYPI LLANRKEDIS NQ