TBP6_YEAST
ID TBP6_YEAST Reviewed; 754 AA.
AC P40328; D6W3U2; P40324; Q02845; Q05773;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Probable 26S proteasome subunit YTA6;
DE AltName: Full=Tat-binding homolog 6;
GN Name=YTA6; OrderedLocusNames=YPL074W; ORFNames=LPF11C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7754704; DOI=10.1002/yea.320100903;
RA Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C.,
RA Vetter I., Feldmann H.;
RT "Identification of a set of yeast genes coding for a novel family of
RT putative ATPases with high similarity to constituents of the 26S protease
RT complex.";
RL Yeast 10:1141-1155(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-85.
RX PubMed=3025612; DOI=10.1128/mcb.6.11.3774-3784.1986;
RA Baker H.V.;
RT "Glycolytic gene expression in Saccharomyces cerevisiae: nucleotide
RT sequence of GCR1, null mutants, and evidence for expression.";
RL Mol. Cell. Biol. 6:3774-3784(1986).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-255, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA56959.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA56959.1; Type=Miscellaneous discrepancy; Note=Rearrangements.; Evidence={ECO:0000305};
CC Sequence=CAA56960.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA56960.1; Type=Miscellaneous discrepancy; Note=Rearrangements.; Evidence={ECO:0000305};
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DR EMBL; X81071; CAA56960.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X81071; CAA56959.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U41849; AAB68264.1; -; Genomic_DNA.
DR EMBL; M14145; AAA66912.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11358.1; -; Genomic_DNA.
DR PIR; S61113; S61113.
DR RefSeq; NP_015251.1; NM_001183888.1.
DR AlphaFoldDB; P40328; -.
DR SMR; P40328; -.
DR BioGRID; 36106; 191.
DR DIP; DIP-4025N; -.
DR IntAct; P40328; 12.
DR MINT; P40328; -.
DR STRING; 4932.YPL074W; -.
DR iPTMnet; P40328; -.
DR MaxQB; P40328; -.
DR PaxDb; P40328; -.
DR PRIDE; P40328; -.
DR TopDownProteomics; P40328; -.
DR EnsemblFungi; YPL074W_mRNA; YPL074W; YPL074W.
DR GeneID; 856031; -.
DR KEGG; sce:YPL074W; -.
DR SGD; S000005995; YTA6.
DR VEuPathDB; FungiDB:YPL074W; -.
DR eggNOG; KOG0740; Eukaryota.
DR GeneTree; ENSGT00940000176549; -.
DR HOGENOM; CLU_000688_15_3_1; -.
DR InParanoid; P40328; -.
DR OMA; YHLKKLM; -.
DR BioCyc; YEAST:G3O-33981-MON; -.
DR PRO; PR:P40328; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P40328; protein.
DR GO; GO:0005938; C:cell cortex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISS:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015415; Vps4_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF09336; Vps4_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..754
FT /note="Probable 26S proteasome subunit YTA6"
FT /id="PRO_0000084759"
FT REGION 219..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 317..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..252
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 253..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..334
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 338..352
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..404
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 511..518
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 255
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 20
FT /note="L -> LQL (in Ref. 4; AAA66912)"
FT /evidence="ECO:0000305"
FT CONFLICT 85
FT /note="D -> I (in Ref. 4; AAA66912)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 754 AA; 85300 MW; 709F33D563382356 CRC64;
MAHEKFSIPE NFTLAQSLQL LYSVVKNQYK NLADLIINSK GNKDTVTYGK IHKNLDTLLV
YVNEGLRKIE KTYTLKKGLG NLVVDHPELR SIIEDFQILG QDIRIARRKA ETLMAEGNGS
PSLSSSSSVL GLGTGNGLRF PKLWRMGSKR DKLKEADEKE AKINKQADNI RRARKLEEEK
KLGAKLQYER DLELQREKLI ELKVKEKVEF EVAQKLEEER VKREEEERKH REQAEKKRIS
TLKHERKTNY KSRASLDNFS SSSKSSGKID NSLIKRRSLD VVRTSGERVR TPVRKSMEAA
EIGMAAQLAW SQYQNGANHS KVSNNGSHSN ELQIRYKPTP PLKKRYDYKK PTVNRPIIKS
PTLNRQNSKS SRNIPTNSKL KASKSNTNKV SRRNEQNLEP SSPVLVSATA VPAESKPMRS
KSGTPDKESS ASSSLDSRKE DILKSVQGVD RNACEQILNE ILVTDEKVYW EDIAGLRNAK
NSLKEAVVYP FLRPDLFKGL REPVRGMLLF GPPGTGKTMI AKAVATESNS TFFSVSASSL
LSKYLGESEK LVRALFYMAK KLSPSIIFID EIDSMLTARS DNENESSRRI KTELLIQWSS
LSSATAQSED RNNTLDSRVL VLGATNLPWA IDDAARRRFS RKLYIPLPDY ETRLYHLKRL
MAKQKNSLQD LDYELITEMT EGFSGSDLTS LAKEAAMEPI RDLGDKLMFA DFDKIRGIEI
KDFQNALLTI KKSVSSESLQ KYEEWSSKFG SNGS
