TBPL1_CAEEL
ID TBPL1_CAEEL Reviewed; 508 AA.
AC B2D6P4; G5EC52; Q9XZQ7;
DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=TATA box-binding protein-like 1 {ECO:0000250|UniProtKB:P62380};
DE Short=TBP-like {ECO:0000305};
DE AltName: Full=TBP-like factor {ECO:0000303|PubMed:10470030};
DE Short=CeTLF {ECO:0000303|PubMed:11030349};
GN Name=tlf-1 {ECO:0000312|WormBase:F39H11.2b};
GN ORFNames=F39H11.2 {ECO:0000312|WormBase:F39H11.2b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAF59926.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=11030349; DOI=10.1016/s1097-2765(00)00068-x;
RA Kaltenbach L., Horner M.A., Rothman J.H., Mango S.E.;
RT "The TBP-like factor CeTLF is required to activate RNA polymerase II
RT transcription during C. elegans embryogenesis.";
RL Mol. Cell 6:705-713(2000).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000312|EMBL:CAB41469.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-457 (ISOFORM A).
RX PubMed=10470030; DOI=10.1016/s0968-0004(99)01436-x;
RA Dantonel J.-C., Wurtz J.-M., Poch O., Moras D., Tora L.;
RT "The TBP-like factor: an alternative transcription factor in metazoa?";
RL Trends Biochem. Sci. 24:335-339(1999).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=11030350; DOI=10.1016/s1097-2765(00)00069-1;
RA Dantonel J.C., Quintin S., Lakatos L., Labouesse M., Tora L.;
RT "TBP-like factor is required for embryonic RNA polymerase II transcription
RT in C. elegans.";
RL Mol. Cell 6:715-722(2000).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=14726532; DOI=10.1074/jbc.m310731200;
RA Walker A.K., Shi Y., Blackwell T.K.;
RT "An extensive requirement for transcription factor IID-specific TAF-1 in
RT Caenorhabditis elegans embryonic transcription.";
RL J. Biol. Chem. 279:15339-15347(2004).
CC -!- FUNCTION: May be a general transcription factor (PubMed:11030350,
CC PubMed:11030349). Plays an essential role for RNA polymerase II/ama-1
CC transcription in early embryos whereby it activates a subset of RNA
CC polymerase II promoters and facilitates the reestablishment of
CC transcription after mitosis (PubMed:14726532).
CC {ECO:0000269|PubMed:11030349, ECO:0000269|PubMed:11030350,
CC ECO:0000269|PubMed:14726532}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11030349,
CC ECO:0000269|PubMed:11030350, ECO:0000269|PubMed:14726532}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:F39H11.2b};
CC IsoId=B2D6P4-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F39H11.2a};
CC IsoId=B2D6P4-2; Sequence=VSP_060675;
CC -!- DEVELOPMENTAL STAGE: Widely expressed throughout embryogenesis,
CC starting at the two-cell stage and probably expressed both maternally
CC and zygotically (at protein level). {ECO:0000269|PubMed:11030349,
CC ECO:0000269|PubMed:11030350}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes early embryonic
CC defects, including premature cell division of the Ea and Ep
CC blastomeres, a failure to undergo gastrulation, and embryonic arrest
CC (PubMed:11030350, PubMed:11030349). Knockdown causes severe loss of
CC polymerase II large subunit ama-1 phosphorylation (PubMed:11030350,
CC PubMed:11030349). Knockdown also causes a reduction in expression
CC levels of several embryonic genes (PubMed:11030350, PubMed:11030349,
CC PubMed:14726532). {ECO:0000269|PubMed:11030349,
CC ECO:0000269|PubMed:11030350, ECO:0000269|PubMed:14726532}.
CC -!- SIMILARITY: Belongs to the TBP family. {ECO:0000305}.
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DR EMBL; AF228692; AAF59926.1; -; mRNA.
DR EMBL; BX284601; CAB03082.2; -; Genomic_DNA.
DR EMBL; BX284601; CAQ35044.1; -; Genomic_DNA.
DR EMBL; AJ238443; CAB41469.1; -; mRNA.
DR PIR; T22000; T22000.
DR RefSeq; NP_001122474.1; NM_001129002.1. [B2D6P4-1]
DR RefSeq; NP_492356.1; NM_059955.4. [B2D6P4-2]
DR AlphaFoldDB; B2D6P4; -.
DR SMR; B2D6P4; -.
DR STRING; 6239.F39H11.2b; -.
DR PaxDb; B2D6P4; -.
DR PeptideAtlas; B2D6P4; -.
DR EnsemblMetazoa; F39H11.2a.1; F39H11.2a.1; WBGene00006577. [B2D6P4-2]
DR EnsemblMetazoa; F39H11.2b.1; F39H11.2b.1; WBGene00006577. [B2D6P4-1]
DR GeneID; 172676; -.
DR KEGG; cel:CELE_F39H11.2; -.
DR UCSC; F39H11.2a; c. elegans. [B2D6P4-1]
DR CTD; 172676; -.
DR WormBase; F39H11.2a; CE27159; WBGene00006577; tlf-1.
DR WormBase; F39H11.2b; CE42480; WBGene00006577; tlf-1. [B2D6P4-1]
DR eggNOG; KOG3302; Eukaryota.
DR GeneTree; ENSGT00940000155712; -.
DR HOGENOM; CLU_038511_0_0_1; -.
DR InParanoid; B2D6P4; -.
DR OMA; IVLEFRC; -.
DR OrthoDB; 1005572at2759; -.
DR PRO; PR:B2D6P4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00006577; Expressed in embryo and 4 other tissues.
DR ExpressionAtlas; B2D6P4; baseline and differential.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0140223; F:general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR CDD; cd04517; TLF; 1.
DR Gene3D; 3.30.310.10; -; 2.
DR InterPro; IPR000814; TBP.
DR InterPro; IPR015445; TBP-like.
DR InterPro; IPR012295; TBP_dom_sf.
DR PANTHER; PTHR10126; PTHR10126; 1.
DR Pfam; PF00352; TBP; 2.
DR PRINTS; PR00686; TIFACTORIID.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..508
FT /note="TATA box-binding protein-like 1"
FT /id="PRO_0000450699"
FT REGION 145..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 456..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 13..15
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_060675"
SQ SEQUENCE 508 AA; 57805 MW; 643DBDE0BE3CB5F8 CRC64;
MQMGDHQMMG NQRYYRTYVQ KVMPAQAGGA VSQNATYVQT AGIRTVHHDG NGQQRIVQLP
PGVRQIQQNG VGPAYVRQVP GGQPMQVNFG HPGTIAGRNV AVGVQMRPVQ GHNVQQGYQR
QQVANQIQQQ NRAVFMAQNQ QGQQQISYAQ AQHRQQQQNQ QQHHQQPQHF NHPSQQNQMI
MQHRQPQMHH NQQHQMVQPQ MTRHQMAQHH AQQPHPQIYV PRDMNLAVPL REPSPEPIPV
KIEVPDVPPE GTSAANEEPM PDDGDIDIQI RNVVCNYTLP LHIDLRKLAM NTHNVTYERE
KGVMMKQKRS PGCYIKVYSS GKVYIVGCRS EADCKRAARS IARHVQRVMG KTKERVSIRN
YRVNNVLATC RLPFGIKIEE VAAKYPSEST YEPELSVGLV WRSVTPKATL RIHTTGSITV
TGAQSEADVL EVLSKIYPIV LEFRCLERAK GNVAAQKKRK RKAPVNRGPP IKRERFDDSN
YRNSGVINNQ VYFSDEDEDL YDELDLEE
