ID   TBP_TOBAC               Reviewed;         200 AA.
AC   P93348;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=TATA-box-binding protein;
DE   AltName: Full=TATA sequence-binding protein;
DE            Short=TBP;
DE   AltName: Full=TATA-binding factor;
DE   AltName: Full=TATA-box factor;
DE   AltName: Full=Transcription initiation factor TFIID TBP subunit;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Bright Yellow 2;
RX   PubMed=9177313; DOI=10.1023/a:1005759521285;
RA   Iwataki N., Hoya A., Yamazaki K.;
RT   "Restoration of TATA-dependent transcription in a heat-inactivated extract
RT   of tobacco nuclei by recombinant TATA-binding protein (TBP) from tobacco.";
RL   Plant Mol. Biol. 34:69-79(1997).
CC   -!- FUNCTION: General transcription factor that functions at the core of
CC       the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA
CC       box is the initial transcriptional step of the pre-initiation complex
CC       (PIC), playing a role in the activation of eukaryotic genes transcribed
CC       by RNA polymerase II.
CC   -!- SUBUNIT: Belongs to the TFIID complex together with the TBP-associated
CC       factors (TAFs). Binds DNA as monomer.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the TBP family. {ECO:0000305}.
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DR   EMBL; D86722; BAA13156.1; -; mRNA.
DR   RefSeq; NP_001312392.1; NM_001325463.1.
DR   RefSeq; XP_016466344.1; XM_016610858.1.
DR   AlphaFoldDB; P93348; -.
DR   SMR; P93348; -.
DR   STRING; 4097.P93348; -.
DR   GeneID; 107789091; -.
DR   KEGG; nta:107789091; -.
DR   OMA; VEYNRRK; -.
DR   PhylomeDB; P93348; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0140223; F:general transcription initiation factor activity; IBA:GO_Central.
DR   GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; IBA:GO_Central.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR   CDD; cd04516; TBP_eukaryotes; 1.
DR   Gene3D; 3.30.310.10; -; 2.
DR   HAMAP; MF_00408; TATA_bind_prot_arch; 1.
DR   InterPro; IPR000814; TBP.
DR   InterPro; IPR030491; TBP_CS.
DR   InterPro; IPR012295; TBP_dom_sf.
DR   InterPro; IPR033710; TBP_eukaryotic.
DR   PANTHER; PTHR10126; PTHR10126; 1.
DR   Pfam; PF00352; TBP; 2.
DR   PRINTS; PR00686; TIFACTORIID.
DR   PROSITE; PS00351; TFIID; 2.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Repeat; Transcription.
FT   CHAIN           1..200
FT                   /note="TATA-box-binding protein"
FT                   /id="PRO_0000153984"
FT   REPEAT          25..101
FT                   /note="1"
FT   REPEAT          115..192
FT                   /note="2"
SQ   SEQUENCE   200 AA;  22302 MW;  59C7EDDB90AE9E1D CRC64;
     MAEVGLEGNQ PVDLSKHPSG IVPTLQNIVS TVNLDCKLDL KAIALQARNA EYNPKRFAAV
     IMRIREPKTT ALIFASGKMV CTGAKSEQSS KLAARKYARI IQKLGFDAKF KDFKIQNIVG
     SCDVKFPIRL EGLAYSHGAF SSYEPELFPG LIYRMKQPKI VLLIFVSGKI VLTGAKVRDE
     TYTAFENIYP VLTEFRKNQQ