TBP_XENLA
ID TBP_XENLA Reviewed; 297 AA.
AC P27633; Q3KPN3;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=TATA-box-binding protein;
DE AltName: Full=TATA sequence-binding protein;
DE AltName: Full=TATA-binding factor;
DE AltName: Full=TATA-box factor;
DE AltName: Full=Transcription initiation factor TFIID TBP subunit;
GN Name=tbp; Synonyms=tfiid;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1641350; DOI=10.1093/nar/20.14.3788;
RA Hashimoto S., Fujita H., Hasegawa S., Roeder R.G., Horikoshi M.;
RT "Conserved structural motifs within the N-terminal domain of TFIID tau from
RT Xenopus, mouse and human.";
RL Nucleic Acids Res. 20:3788-3788(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Oocyte;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PHOSPHORYLATION.
RX PubMed=8647263; DOI=10.1016/0014-5793(96)00420-6;
RA Labhart P.;
RT "Phosphorylation of the N-terminal domain of Xenopus TATA-box binding
RT protein by DNA-dependent protein kinase depends on the C-terminal core
RT domain.";
RL FEBS Lett. 386:110-114(1996).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16412700; DOI=10.1016/j.modgep.2005.09.005;
RA Xiao L., Kim M., DeJong J.;
RT "Developmental and cell type-specific regulation of core promoter
RT transcription factors in germ cells of frogs and mice.";
RL Gene Expr. Patterns 6:409-419(2006).
RN [5]
RP FUNCTION.
RX PubMed=17703192; DOI=10.1038/sj.emboj.7601822;
RA Jacobi U.G., Akkers R.C., Pierson E.S., Weeks D.L., Dagle J.M.,
RA Veenstra G.J.C.;
RT "TBP paralogs accommodate metazoan- and vertebrate-specific developmental
RT gene regulation.";
RL EMBO J. 26:3900-3909(2007).
CC -!- FUNCTION: General transcription factor that functions at the core of
CC the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA
CC box is the initial transcriptional step of the pre-initiation complex
CC (PIC), playing a role in the activation of eukaryotic genes transcribed
CC by RNA polymerase II. Members of the TBP family are differentially
CC required to regulate transcription and development during early
CC embryogenesis. Binds to the promoters of select genes.
CC {ECO:0000269|PubMed:17703192}.
CC -!- SUBUNIT: Belongs to the TFIID complex together with the TBP-associated
CC factors (TAFs). Binds DNA as monomer. {ECO:0000250|UniProtKB:P20226}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P20226}.
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously with highest expression in
CC the ovary and testis. {ECO:0000269|PubMed:16412700}.
CC -!- PTM: The N-terminal domain is extensively phosphorylated.
CC {ECO:0000269|PubMed:8647263}.
CC -!- SIMILARITY: Belongs to the TBP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X66033; CAA46832.1; -; mRNA.
DR EMBL; BC106644; AAI06645.1; -; mRNA.
DR PIR; I51648; I51648.
DR RefSeq; NP_001084369.1; NM_001090900.1.
DR AlphaFoldDB; P27633; -.
DR SMR; P27633; -.
DR BioGRID; 100789; 1.
DR MINT; P27633; -.
DR DNASU; 399465; -.
DR GeneID; 399465; -.
DR KEGG; xla:399465; -.
DR CTD; 399465; -.
DR Xenbase; XB-GENE-6253887; tbp.L.
DR OrthoDB; 1219067at2759; -.
DR Proteomes; UP000186698; Chromosome 5L.
DR Bgee; 399465; Expressed in blastula and 19 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; ISS:UniProtKB.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:UniProtKB.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006383; P:transcription by RNA polymerase III; ISS:UniProtKB.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; NAS:UniProtKB.
DR CDD; cd04516; TBP_eukaryotes; 1.
DR Gene3D; 3.30.310.10; -; 2.
DR HAMAP; MF_00408; TATA_bind_prot_arch; 1.
DR InterPro; IPR000814; TBP.
DR InterPro; IPR030491; TBP_CS.
DR InterPro; IPR012295; TBP_dom_sf.
DR InterPro; IPR033710; TBP_eukaryotic.
DR PANTHER; PTHR10126; PTHR10126; 1.
DR Pfam; PF00352; TBP; 2.
DR PRINTS; PR00686; TIFACTORIID.
DR PROSITE; PS00351; TFIID; 2.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..297
FT /note="TATA-box-binding protein"
FT /id="PRO_0000153962"
FT REPEAT 123..199
FT /note="1"
FT REPEAT 213..290
FT /note="2"
FT REGION 52..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..107
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 297 AA; 32703 MW; 9893593418AEFD81 CRC64;
MDQNNSIPPF QGLASPQGSL TPGINIFSPL ITYGTGLTPQ PVQTTNSLSI LEEQQRQQQQ
TQQSTLQQGN QGSGQTPQLF HPQTLTTAPL PGNTPLYPSP ITPMTPISPA TPASESSGIV
PQLQNIVSTV NLGCKLDLKT IALRARNAEY NPKRFAAVIM RIREPRTTAL IFSSGKMVCT
GAKSEEQSRL AARKYARVVQ KLGFPAKFLD FKIQNMVGSC DVKFPIRLEG LVLTHQQFSS
YEPELFPGLI YRMIKPRIVL LIFVSGKVVL TGAKVRAEIY EAFENIYPIL KGFRKTT
