TBT2_ORYSJ
ID TBT2_ORYSJ Reviewed; 448 AA.
AC Q2R0J4;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Tryptamine benzoyltransferase 2 {ECO:0000305};
DE Short=OsTBT2 {ECO:0000303|PubMed:27354554};
DE EC=2.3.1.- {ECO:0000305};
GN Name=TBT2 {ECO:0000303|PubMed:27354554};
GN OrderedLocusNames=Os11g0643100 {ECO:0000312|EMBL:BAT15024.1},
GN LOC_Os11g42370 {ECO:0000312|EMBL:ABA95035.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=27354554; DOI=10.1105/tpc.16.00265;
RA Peng M., Gao Y., Chen W., Wang W., Shen S., Shi J., Wang C., Zhang Y.,
RA Zou L., Wang S., Wan J., Liu X., Gong L., Luo J.;
RT "Evolutionarily distinct BAHD N-acyltransferases are responsible for
RT natural variation of aromatic amine conjugates in rice.";
RL Plant Cell 28:1533-1550(2016).
CC -!- FUNCTION: Hydroxycinnamoyl transferase that catalyzes the transfer of
CC an acyl from benzoyl-CoA to tryptamine, to produce benzoyl tryptamine.
CC Serotonin and tyramine serve as acyl acceptors in vitro. Specific for
CC benzoyl-CoA as acyl donor. Has no activity with p-coumaroyl-CoA,
CC caffeoyl-CoA, or feruloyl-CoA as acyl donors.
CC {ECO:0000269|PubMed:27354554}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=81.3 uM for benzoyl-CoA {ECO:0000269|PubMed:27354554};
CC KM=45.4 uM for tryptamine {ECO:0000269|PubMed:27354554};
CC KM=37.5 uM for serotonin {ECO:0000269|PubMed:27354554};
CC KM=114.3 uM for tyramine {ECO:0000269|PubMed:27354554};
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; DP000010; ABA95035.1; -; Genomic_DNA.
DR EMBL; AP008217; BAF28721.1; -; Genomic_DNA.
DR EMBL; AP014967; BAT15024.1; -; Genomic_DNA.
DR EMBL; AK061327; BAG87858.1; -; mRNA.
DR RefSeq; XP_015615816.1; XM_015760330.1.
DR AlphaFoldDB; Q2R0J4; -.
DR SMR; Q2R0J4; -.
DR STRING; 4530.OS11T0643100-01; -.
DR PaxDb; Q2R0J4; -.
DR PRIDE; Q2R0J4; -.
DR EnsemblPlants; Os11t0643100-01; Os11t0643100-01; Os11g0643100.
DR GeneID; 4351010; -.
DR Gramene; Os11t0643100-01; Os11t0643100-01; Os11g0643100.
DR KEGG; osa:4351010; -.
DR eggNOG; ENOG502QVP8; Eukaryota.
DR HOGENOM; CLU_014546_6_0_1; -.
DR InParanoid; Q2R0J4; -.
DR OMA; FCTTWAR; -.
DR OrthoDB; 1130893at2759; -.
DR SABIO-RK; Q2R0J4; -.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR ExpressionAtlas; Q2R0J4; baseline and differential.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0050734; F:hydroxycinnamoyltransferase activity; IDA:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..448
FT /note="Tryptamine benzoyltransferase 2"
FT /id="PRO_0000437769"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 155
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT ACT_SITE 386
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
SQ SEQUENCE 448 AA; 48023 MW; 2D03D3CFF7F15132 CRC64;
MEITSSAMLK PAPTPTPHPL AGEKVPLTAF DRAAFDVFVP MVFAYRAPAP SSEAVKEGLR
MAVAAYPLAA GRLAVDVAAD GQGRRRRRRV LHVNDEGALV LDATVEADLD AVLAANVATD
LYPAPPEHSF GAAVLQVQLT RFRCSGLVVG LIVHHHVFDG HSTSAFCTTW ARAVRDGEAF
SVPSPCLDRA ITSVPRSPPA PVFDHRSIEF KVGNKSSDSS GAAAAAVEKI TNIGVRFTAK
FVAELKARVG GRCSTFECVL AHAWKKMTAA RGLKPEEFTR VRVAVNCRRR ANPPAPADLF
GNMVLWAFPR LQVRRLLSAS YRDVVGAIRA AVARVDGEYI QSFVDYVEVA DARGEELAAT
AAEPGETLCP DLEVDSWLGF RFHEMDLGTG PPAAVLSPDL PIEGLMILVP VGGDGGGVDL
FVALADDRAQ VFEQICYSLE EHAIPSHL
