TBX12_CAEEL
ID TBX12_CAEEL Reviewed; 346 AA.
AC P90971; O17276;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=T-box protein 12;
DE AltName: Full=Protein male abnormal 9;
GN Name=mab-9; Synonyms=tbx-12; ORFNames=T27A1.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9276934; DOI=10.1139/g97-061;
RA Agulnik S.I., Ruvinsky I., Silver L.M.;
RT "Three novel T-box genes in Caenorhabditis elegans.";
RL Genome 40:458-464(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=10716947;
RA Woollard A., Hodgkin J.;
RT "The Caenorhabditis elegans fate-determining gene mab-9 encodes a T-box
RT protein required to pattern the posterior hindgut.";
RL Genes Dev. 14:596-603(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF GLY-103.
RX PubMed=28056346; DOI=10.1016/j.neuron.2016.11.036;
RA Kerk S.Y., Kratsios P., Hart M., Mourao R., Hobert O.;
RT "Diversification of C. elegans Motor Neuron Identity via Selective Effector
RT Gene Repression.";
RL Neuron 93:80-98(2017).
CC -!- FUNCTION: Transcription factor (PubMed:28056346). Involved in cell fate
CC determination; required to pattern the posterior hindgut
CC (PubMed:10716947). Involved in motor neuron fate determination and
CC maintenance, acting as a transcriptional repressor to counteract gene
CC activation by transcription factor unc-3 in a subset of motor neurons
CC (PubMed:28056346). Required throughout development to repress
CC transcription by unc-3, probably acting by binding to specific promoter
CC elements (PubMed:28056346). Represses expression of VA and VB motor
CC neuron-specific effector genes, such as DEG/ENaC channel del-1 and the
CC innexin inx-12, in DA and DB motor neurons (PubMed:28056346). Represses
CC expression of transcription factor bnc-1, perhaps acting directly, in
CC DA and DB motor neurons (PubMed:28056346).
CC {ECO:0000269|PubMed:10716947, ECO:0000269|PubMed:28056346}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201,
CC ECO:0000269|PubMed:10716947}.
CC -!- DEVELOPMENTAL STAGE: First expressed during embryogenesis at the 1.5-
CC fold stage, and in very few cells throughout development
CC (PubMed:10716947). Expressed in L1 larvae in the posterior hindgut
CC cells B and F and in later L1 stage in the B daughter cells B.a and B.p
CC (PubMed:10716947). Also expressed in two posterior hypodermal nuclei of
CC the large syncytial cell hyp7 and in the nervous system in two neurons
CC of the lumbar ganglion (PubMed:10716947).
CC {ECO:0000269|PubMed:10716947}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown by injection into the
CC gonad results in male progeny with grossly abnormal tails with no
CC spicules (PubMed:10716947). Hermaphrodite progeny are constipated and
CC show backward uncoordination (PubMed:10716947).
CC {ECO:0000269|PubMed:10716947}.
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DR EMBL; U56081; AAB37243.1; -; mRNA.
DR EMBL; AJ252168; CAB65731.1; -; mRNA.
DR EMBL; FO081751; CCD73630.1; -; Genomic_DNA.
DR PIR; A88023; A88023.
DR RefSeq; NP_493750.1; NM_061349.6.
DR AlphaFoldDB; P90971; -.
DR SMR; P90971; -.
DR BioGRID; 38818; 3.
DR IntAct; P90971; 3.
DR STRING; 6239.T27A1.6; -.
DR PaxDb; P90971; -.
DR EnsemblMetazoa; T27A1.6.1; T27A1.6.1; WBGene00003106.
DR GeneID; 173441; -.
DR KEGG; cel:CELE_T27A1.6; -.
DR UCSC; T27A1.6; c. elegans.
DR CTD; 173441; -.
DR WormBase; T27A1.6; CE24016; WBGene00003106; mab-9.
DR eggNOG; KOG3586; Eukaryota.
DR GeneTree; ENSGT00940000168440; -.
DR HOGENOM; CLU_014430_2_0_1; -.
DR InParanoid; P90971; -.
DR OMA; ERSPGDM; -.
DR OrthoDB; 1185429at2759; -.
DR PhylomeDB; P90971; -.
DR SignaLink; P90971; -.
DR PRO; PR:P90971; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003106; Expressed in larva and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001708; P:cell fate specification; IMP:WormBase.
DR GO; GO:0007507; P:heart development; IBA:GO_Central.
DR GO; GO:0040011; P:locomotion; IMP:WormBase.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0048620; P:post-embryonic hindgut morphogenesis; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..346
FT /note="T-box protein 12"
FT /id="PRO_0000184474"
FT DNA_BIND 86..268
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 33..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..47
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 103
FT /note="G->R: In ot720; abolishes unc-129 expression in DA
FT and DB motor neurons. Causes ectopic expression of
FT transcription factor bnc-1 in DA and DB motor neurons."
FT /evidence="ECO:0000269|PubMed:28056346"
FT CONFLICT 296
FT /note="Q -> R (in Ref. 1; AAB37243)"
FT /evidence="ECO:0000305"
FT CONFLICT 331
FT /note="A -> G (in Ref. 1; AAB37243)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 346 AA; 39435 MW; 899F0788DEB9FD4D CRC64;
MSKRCASDRD DKDSEPVKKP RFSIANILDE VEDEEDVEVD VEDVDDVDLS SIPSKSPERS
RGRPKIGLKM KEGNLPIECK LEGSELWAKF FDLGTEMIIT KSGRRMFPTV KVSFTNVILD
ALYYIFLDVV PVDSKRYRYI YNKSAWLTAG KAEPVPKNRY YLHPDSPFTG DQLLKHVISF
EKTKLTNNEV DKTGHLILNS MHKYQPRIHI VQRQKANPLD PNKVVMSEEK HCTYTFPETQ
FMAVTAYQNQ LITKLKIEKN PFAKGFRDPT GRSPDEMERS PGDMMLSNFY HSSALQQAMF
QQCLSKTLQL NPSIMMLYQN VFPTGNSLPA APTVPGNPAE ISIKSE
