TBX2_DANRE
ID TBX2_DANRE Reviewed; 687 AA.
AC Q7ZTU9; Q9IAL0; Q9W7B7;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 4.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=T-box transcription factor TBX2b;
DE Short=T-box protein 2b;
GN Name=tbx2b; Synonyms=tbx-c;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=10664151; DOI=10.1007/s004270050014;
RA Ruvinsky I., Oates A.C., Silver L.M., Ho R.K.;
RT "The evolution of paired appendages in vertebrates: T-box genes in the
RT zebrafish.";
RL Dev. Genes Evol. 210:82-91(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=SJD;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-682, FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=10331981; DOI=10.1242/dev.126.12.2703;
RA Dheen T., Sleptsova-Friedrich I., Xu Y., Clark M., Lehrach H., Gong Z.,
RA Korzh V.;
RT "Zebrafish tbx-c functions during formation of midline structures.";
RL Development 126:2703-2713(1999).
RN [4]
RP FUNCTION IN HEART DEVELOPMENT, AND DISRUPTION PHENOTYPE.
RX PubMed=18347092; DOI=10.1101/gad.1629408;
RA Chi N.C., Shaw R.M., De Val S., Kang G., Jan L.Y., Black B.L.,
RA Stainier D.Y.;
RT "Foxn4 directly regulates tbx2b expression and atrioventricular canal
RT formation.";
RL Genes Dev. 22:734-739(2008).
CC -!- FUNCTION: Transcription factor which acts as a transcriptional
CC repressor (By similarity). May also function as a transcriptional
CC activator (By similarity). Binds to the palindromic T site 5'-
CC TTCACACCTAGGTGTGAA-3' DNA sequence, or a half-site, which are present
CC in the regulatory region of several genes (By similarity). Involved in
CC the transcriptional regulation of genes required for mesoderm
CC differentiation. Plays a role in the specification of late notochordal
CC precursor cells and formation of the differentiated notochord. Required
CC for cardiac atrioventricular canal formation.
CC {ECO:0000250|UniProtKB:Q13207, ECO:0000250|UniProtKB:Q60707,
CC ECO:0000269|PubMed:10331981, ECO:0000269|PubMed:18347092}.
CC -!- SUBUNIT: Binds DNA as a monomer. {ECO:0000250|UniProtKB:Q13207}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13207}.
CC -!- TISSUE SPECIFICITY: Expressed in the axial mesoderm, notably, in the
CC notochordal precursor cells immediately before formation of the
CC notochord and in the chordoneural hinge of the tail bud, after the
CC notochord is formed. In addition, its expression is detected in the
CC ventral forebrain, sensory neurons, fin buds and excretory system.
CC {ECO:0000269|PubMed:10331981}.
CC -!- DEVELOPMENTAL STAGE: Expression seen in many tissues of ectodermal and
CC mesodermal origin during embryogenesis. First detected at bud stage in
CC the ventral prosencephalon and along the length of the notochord. As
CC segmentation proceeds, expressed in the optic primordia, otic placodes
CC and diffusely in cells over the yolk lying ventrally to the tail bud.
CC Midway through the segmentation period, expression also seen in the
CC acostic and lateral line sensory ganglia. At 22 hpf, found in the most
CC posterior portion of the pronephric ducts (PubMed:10664151). At the end
CC of gastrulation, expression seen in the prospective ventral forebrain,
CC single eye field and the presumptive notochord. At 12 hpf, expression
CC in the midline found only at the posterior end of the notochord where
CC it can be detected until the end of segmentation. At 20 hpf, expression
CC in the eye is restricted to the dorsal retina and also found in the
CC epiphysis, otic vesicles and sensory neurons of cranial ganglia. At 22
CC hpf, expressed in bud of pectoral fins and at 30 hpf, in a subset of
CC interneurons in the anterior spinal cord (PubMed:10331981).
CC {ECO:0000269|PubMed:10331981, ECO:0000269|PubMed:10664151}.
CC -!- DISRUPTION PHENOTYPE: Loss of atrioventricular canal formation and
CC cardiac looping. {ECO:0000269|PubMed:18347092}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD44194.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF179405; AAF59835.1; -; mRNA.
DR EMBL; BC051603; AAH51603.2; -; mRNA.
DR EMBL; AF136946; AAD44194.1; ALT_FRAME; mRNA.
DR RefSeq; NP_571126.1; NM_131051.1.
DR AlphaFoldDB; Q7ZTU9; -.
DR SMR; Q7ZTU9; -.
DR STRING; 7955.ENSDARP00000107769; -.
DR PaxDb; Q7ZTU9; -.
DR DNASU; 30253; -.
DR Ensembl; ENSDART00000005491; ENSDARP00000020624; ENSDARG00000116135.
DR Ensembl; ENSDART00000122101; ENSDARP00000107769; ENSDARG00000006120.
DR GeneID; 30253; -.
DR KEGG; dre:30253; -.
DR CTD; 30253; -.
DR ZFIN; ZDB-GENE-990726-27; tbx2b.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00940000158439; -.
DR HOGENOM; CLU_023038_1_0_1; -.
DR InParanoid; Q7ZTU9; -.
DR OMA; HQDERCT; -.
DR OrthoDB; 301173at2759; -.
DR PhylomeDB; Q7ZTU9; -.
DR TreeFam; TF106341; -.
DR PRO; PR:Q7ZTU9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 15.
DR Bgee; ENSDARG00000006120; Expressed in camera-type eye and 19 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0036302; P:atrioventricular canal development; IGI:ZFIN.
DR GO; GO:0048318; P:axial mesoderm development; IDA:ZFIN.
DR GO; GO:0030509; P:BMP signaling pathway; IMP:ZFIN.
DR GO; GO:0007420; P:brain development; IMP:ZFIN.
DR GO; GO:0060219; P:camera-type eye photoreceptor cell differentiation; IMP:ZFIN.
DR GO; GO:0003205; P:cardiac chamber development; IGI:ZFIN.
DR GO; GO:0007155; P:cell adhesion; IMP:ZFIN.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:ZFIN.
DR GO; GO:0035050; P:embryonic heart tube development; IMP:ZFIN.
DR GO; GO:0021538; P:epithalamus development; IMP:ZFIN.
DR GO; GO:0001947; P:heart looping; IMP:ZFIN.
DR GO; GO:0072068; P:late distal convoluted tubule development; IMP:ZFIN.
DR GO; GO:0048663; P:neuron fate commitment; IMP:ZFIN.
DR GO; GO:0030903; P:notochord development; IMP:ZFIN.
DR GO; GO:0042461; P:photoreceptor cell development; IMP:ZFIN.
DR GO; GO:0043704; P:photoreceptor cell fate specification; IMP:ZFIN.
DR GO; GO:0021982; P:pineal gland development; IMP:ZFIN.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0035777; P:pronephric distal tubule development; IMP:ZFIN.
DR GO; GO:0048793; P:pronephros development; IMP:ZFIN.
DR GO; GO:0072019; P:proximal convoluted tubule development; IMP:ZFIN.
DR GO; GO:0008016; P:regulation of heart contraction; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0046549; P:retinal cone cell development; IMP:ZFIN.
DR GO; GO:0046551; P:retinal cone cell fate commitment; IMP:ZFIN.
DR GO; GO:0042671; P:retinal cone cell fate determination; IMP:ZFIN.
DR GO; GO:0046548; P:retinal rod cell development; IMP:ZFIN.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR022582; TBX2/3_TAD.
DR InterPro; IPR002070; TF_Brachyury.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR Pfam; PF12598; TBX; 1.
DR PRINTS; PR00938; BRACHYURY.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..687
FT /note="T-box transcription factor TBX2b"
FT /id="PRO_0000262463"
FT DNA_BIND 103..276
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 303..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 611..687
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 654..681
FT /evidence="ECO:0000255"
FT COMPBIAS 336..357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..428
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 429..452
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 611..632
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 642..679
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 368
FT /note="E -> K (in Ref. 1; AAF59835)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 687 AA; 75335 MW; AC57238D05744256 CRC64;
MRDPVFTGTA MAYHPFHAHR PTDFPMSAFL AAAQPSFFPA LTLPPGALTK PIPDHTLAGA
AEAGLHPALS HHHQAAHLRS LKSLEPEEEV EDDPKVTLEA KDLWDQFHKL GTEMVITKSG
RRMFPPFKVR INGLDKKAKY ILLMDIVAAD DCRYKFHNSR WMVAGKADPE MPKRMYIHPD
SPATGEQWMA KPVAFHKLKL TNNISDKHGF TILNSMHKYQ PRFHIVRAND ILKLPYSTFR
TYVFPETDFI AVTAYQNDKI TQLKIDNNPF AKGFRDTGNG RREKRKQLTL PSLRMYEDQC
KVDRDGADSD ASSSEPTTGR DAGHSPGPVS SPLRFNRGSR DDKTCTDSEH EMDHQNDRCG
GSSSPAPEPS SPFRSRSEDW GREKPIAEKK DDYPDSRKTS DSIFSIRNLE KDKLESRSRK
DTDSSKKDTE NSGISGSKDS FSPLMVQTES PSHFGAGHLQ SLALSGLHSQ QFFNPLNTGQ
PLLFHPGQFA MAPGAFSAMG MGHLLASVSG AGGLENGSLS AQGTGSTPSP FPFHLSQHML
ASQGIPMPTF GGLFPYPYTY MAAAAAAASA LPASSSTASS LSRNPFLSSS TRPRLRFNPY
QLPVSIPQST NLLTTGLPSG LNPSSESSKC GSREASPVPD HKSGASQRNG SPKTTMKESI
NELQNIQRLV SGLESQRETS SPRDSPK
