TBX4_MOUSE
ID TBX4_MOUSE Reviewed; 552 AA.
AC P70325; B1AQT9; Q8R5F6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=T-box transcription factor TBX4;
DE Short=T-box protein 4;
GN Name=Tbx4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/SvEv;
RA Arruda E.P., Bruneau B.G.;
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 92-265.
RC TISSUE=Embryo;
RX PubMed=8878690; DOI=10.1093/genetics/144.1.249;
RA Agulnik S.I., Garvey N., Hancock S., Ruvinsky I., Chapman D.L., Agulnik I.,
RA Bollag R.J., Papaioannou V.E., Silver L.M.;
RT "Evolution of mouse T-box genes by tandem duplication and cluster
RT dispersion.";
RL Genetics 144:249-254(1996).
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=8853987;
RX DOI=10.1002/(sici)1097-0177(199608)206:4<379::aid-aja4>3.0.co;2-f;
RA Chapman D.L., Garvey N., Hancock S., Alexiou M., Agulnik S.I.,
RA Gibson-Brown J.J., Cebra-Thomas J., Bollag R.J., Silver L.M.,
RA Papaioannou V.E.;
RT "Expression of the T-box family genes, Tbx1-Tbx5, during early mouse
RT development.";
RL Dev. Dyn. 206:379-390(1996).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-514, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transcriptional regulator that has an essential role in the
CC organogenesis of lungs, pelvis, and hindlimbs.
CC {ECO:0000250|UniProtKB:P57082}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201}.
CC -!- DEVELOPMENTAL STAGE: First expressed at day 7.5, exclusively in the
CC allantois where expression continues through day 8.5. At day 9.5,
CC expression is found in the genital papilla, body wall and limb buds
CC (higher levels in hindlimb). At day 12.5, expressed in the mesenchyme
CC of the mandibular arch, of the lung and of that surrounding the
CC trachea. Also found in the sinus venosus/common atrium of the
CC developing heart. {ECO:0000269|PubMed:8853987}.
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DR EMBL; AY075134; AAL77209.1; -; mRNA.
DR EMBL; AL592465; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466556; EDL15777.1; -; Genomic_DNA.
DR EMBL; U57329; AAC53108.1; -; mRNA.
DR CCDS; CCDS25196.1; -.
DR PIR; S72230; S72230.
DR RefSeq; NP_035666.2; NM_011536.2.
DR RefSeq; XP_006532843.1; XM_006532780.2.
DR AlphaFoldDB; P70325; -.
DR SMR; P70325; -.
DR BioGRID; 203989; 1.
DR IntAct; P70325; 1.
DR STRING; 10090.ENSMUSP00000103680; -.
DR iPTMnet; P70325; -.
DR PhosphoSitePlus; P70325; -.
DR MaxQB; P70325; -.
DR PaxDb; P70325; -.
DR PRIDE; P70325; -.
DR ProteomicsDB; 263138; -.
DR Antibodypedia; 18589; 152 antibodies from 24 providers.
DR DNASU; 21387; -.
DR Ensembl; ENSMUST00000000096; ENSMUSP00000000096; ENSMUSG00000000094.
DR Ensembl; ENSMUST00000108047; ENSMUSP00000103682; ENSMUSG00000000094.
DR GeneID; 21387; -.
DR KEGG; mmu:21387; -.
DR UCSC; uc007ksc.2; mouse.
DR CTD; 9496; -.
DR MGI; MGI:102556; Tbx4.
DR VEuPathDB; HostDB:ENSMUSG00000000094; -.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00940000158882; -.
DR InParanoid; P70325; -.
DR OMA; GNHQTLQ; -.
DR OrthoDB; 344556at2759; -.
DR PhylomeDB; P70325; -.
DR BioGRID-ORCS; 21387; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Tbx4; mouse.
DR PRO; PR:P70325; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P70325; protein.
DR Bgee; ENSMUSG00000000094; Expressed in secondary oocyte and 110 other tissues.
DR ExpressionAtlas; P70325; baseline and differential.
DR Genevisible; P70325; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001525; P:angiogenesis; IMP:MGI.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0035116; P:embryonic hindlimb morphogenesis; ISS:UniProtKB.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:MGI.
DR GO; GO:1990401; P:embryonic lung development; ISS:UniProtKB.
DR GO; GO:0035108; P:limb morphogenesis; IDA:MGI.
DR GO; GO:0030324; P:lung development; IMP:MGI.
DR GO; GO:0002009; P:morphogenesis of an epithelium; IMP:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048705; P:skeletal system morphogenesis; ISS:UniProtKB.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..552
FT /note="T-box transcription factor TBX4"
FT /id="PRO_0000184434"
FT DNA_BIND 76..256
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 514
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 66..67
FT /note="EQ -> DE (in Ref. 1; AAL77209)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="Missing (in Ref. 4; AAC53108)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="S -> R (in Ref. 1; AAL77209)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="A -> G (in Ref. 1; AAL77209)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 552 AA; 61101 MW; 6F4E24171A1B4716 CRC64;
MLQDKGLSES EEAFRAPGPA LGEASNTSTT NAPEPALATP GLSGAALSSP PGQGADVAAA
AAAAAEQTIE NIKVGLHEKE LWKKFHEAGT EMIITKAGRR MFPSYKVKVT GMNPKTKYIL
LIDIVPADDH RYKFCDNKWM VAGKAEPAMP GRLYVHPDSP ATGAHWMRQL VSFQKLKLTN
NHLDPFGHII LNSMHKYQPR LHIVKADENN AFGSKNTAFC THVFPETSFI SVTSYQNHKI
TQLKIENNPF AKGFRGSDDS DLRVARLQSK EYPVISKSIM RQRLVSSQLS AKPDVSPLHS
AHQALQHYQY ENGAHMQFAA AEPQDLPLNT FPTQRDSSLF YHCLKRRDSA RHLDLPCKRS
YLETPSSVGD DHYFRSPPPY DQQMLSPSYC SEVTPREACM YSSSGPEIAG VSAVDDLPPP
PLSCNMWTSV SPYTSYSVQT METVPYQPFP AHFTATTVMP RLPTIAAQSA QPPGNAHFSV
YNQLSQSQVR ERGPSASFPR ERGLPGMCER KPPSPHLNTA NEFLYSQSFS LTRESSLQYH
SGMGTVENWT DG
