TBX5_CHICK
ID TBX5_CHICK Reviewed; 521 AA.
AC Q9PWE8; O73719;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=T-box transcription factor TBX5;
DE Short=T-box protein 5;
GN Name=TBX5;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=9550719; DOI=10.1242/dev.125.10.1867;
RA Issac A., Rodriguez-Esteban C., Ryan A., Altabef M., Tsukui T., Patel K.,
RA Tickle C., Izpisua-Belmonte J.-C.;
RT "Tbx genes and limb identity in chick embryo development.";
RL Development 125:1867-1875(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=10373308; DOI=10.1006/dbio.1999.9298;
RA Bruneau B.G., Logan M., Davis N., Levi T., Tabin C.J., Seidman J.G.,
RA Seidman C.E.;
RT "Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram
RT syndrome.";
RL Dev. Biol. 211:100-108(1999).
CC -!- FUNCTION: DNA-binding protein that regulates the transcription of
CC several genes and is involved in heart development and limb pattern
CC formation. May bind to the core DNA motif of promoters.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SUBUNIT: Monomer. Homodimer (via the T-box); binds DNA as homodimer.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- INTERACTION:
CC Q9PWE8; Q679P3: PDLIM7; NbExp=4; IntAct=EBI-6663870, EBI-6693710;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q99593,
CC ECO:0000255|PROSITE-ProRule:PRU00201}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q99593}. Note=Shuttles between the cytoplasm and
CC the nucleus. {ECO:0000250|UniProtKB:Q99593}.
CC -!- DEVELOPMENTAL STAGE: Early in the developing heart, uniformly expressed
CC throughout the entire cardiac crescent. Upon formation of the linear
CC heart tube, expressed in a graded fashion, stronger near the posterior
CC end and weaker at the anterior end. As the heart tube loops, asymmetric
CC expression continues; it is expressed in the presumptive left
CC ventricle, but not the right ventricle or outflow tract. This pattern
CC of expression is maintained in more mature hearts (PubMed:10373308).
CC First detected in the presumptive wing and leg mesoderm respectively at
CC around stages 14-15. As limb outgrowth proceeds, expressed throughout
CC the mesoderm and in the wing (PubMed:9550719).
CC {ECO:0000269|PubMed:10373308, ECO:0000269|PubMed:9550719}.
CC -!- DOMAIN: The T-Box domain binds to double-stranded DNA.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC41299.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF033671; AAC41299.1; ALT_FRAME; mRNA.
DR EMBL; AF069396; AAC23683.2; -; mRNA.
DR RefSeq; NP_989504.1; NM_204173.1.
DR RefSeq; XP_015150318.1; XM_015294832.1.
DR AlphaFoldDB; Q9PWE8; -.
DR SMR; Q9PWE8; -.
DR IntAct; Q9PWE8; 1.
DR STRING; 9031.ENSGALP00000013419; -.
DR PaxDb; Q9PWE8; -.
DR Ensembl; ENSGALT00000013434; ENSGALP00000013419; ENSGALG00000008253.
DR GeneID; 373988; -.
DR KEGG; gga:373988; -.
DR CTD; 6910; -.
DR VEuPathDB; HostDB:geneid_373988; -.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00940000156506; -.
DR HOGENOM; CLU_037025_1_0_1; -.
DR InParanoid; Q9PWE8; -.
DR OMA; GVANHGS; -.
DR OrthoDB; 344556at2759; -.
DR PhylomeDB; Q9PWE8; -.
DR TreeFam; TF106341; -.
DR PRO; PR:Q9PWE8; -.
DR Proteomes; UP000000539; Chromosome 15.
DR Bgee; ENSGALG00000008253; Expressed in heart and 2 other tissues.
DR ExpressionAtlas; Q9PWE8; baseline and differential.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:AgBase.
DR GO; GO:0005884; C:actin filament; IDA:AgBase.
DR GO; GO:0005737; C:cytoplasm; IDA:AgBase.
DR GO; GO:0005634; C:nucleus; IDA:AgBase.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0032993; C:protein-DNA complex; ISS:UniProtKB.
DR GO; GO:0001725; C:stress fiber; IDA:AgBase.
DR GO; GO:0051087; F:chaperone binding; IPI:AgBase.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0030274; F:LIM domain binding; IPI:AgBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0097110; F:scaffold protein binding; IPI:AgBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0048513; P:animal organ development; ISS:UniProtKB.
DR GO; GO:0003218; P:cardiac left ventricle formation; IEP:BHF-UCL.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; ISS:UniProtKB.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; IMP:AgBase.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISS:UniProtKB.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0035137; P:hindlimb morphogenesis; TAS:AgBase.
DR GO; GO:0060174; P:limb bud formation; IMP:AgBase.
DR GO; GO:0060173; P:limb development; TAS:AgBase.
DR GO; GO:0060044; P:negative regulation of cardiac muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0007389; P:pattern specification process; IBA:GO_Central.
DR GO; GO:0060039; P:pericardium development; ISS:UniProtKB.
DR GO; GO:0051891; P:positive regulation of cardioblast differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:AgBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:AgBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0032526; P:response to retinoic acid; TAS:AgBase.
DR GO; GO:0003281; P:ventricular septum development; IEP:BHF-UCL.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..521
FT /note="T-box transcription factor TBX5"
FT /id="PRO_0000262467"
FT DNA_BIND 63..238
FT /note="T-box"
FT /evidence="ECO:0000250|UniProtKB:Q99593,
FT ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 254..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..45
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 262..281
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..346
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 521 AA; 58402 MW; 17633D0F1DBCE30B CRC64;
MADTEEGFGL PSTPVDSEAK ELQAEAKQDP QLGTTSKAPT SPQAAFTQQG MEGIKVFLHE
RELWLKFHEV GTEMIITKAG RRMFPSYKVK VTGLNPKTKY ILLMDIVPAD DHRYKFADNK
WSVTGKAEPA MPGRLYVHPD SPATGAHWMR QLVSFQKLKL TNNHLDPFGH IILNSMHKYQ
PRLHIVKADE NNGFGSKNTA FCTHVFPETA FIAVTSYQNH KITQLKIENN PFAKGFRGSD
DMELHRMSRM QSKEYPVVPR STVRQKVSSN HSPFSGETRV LSTSSNLGSQ YQCENGVSST
SQDLLPPTNP YPISQEHSQI YHCTKRKDEE CSTTEHPYKK PYMETSPAEE DPFYRSSYPQ
QQGLNTSYRT ESAQRQACMY ASSAPPTDPV PSLEDISCNT WPSVPSYSSC TVSAMQPMDR
LPYQHFSAHF TSGPLMPRLS SVANHTSPQI GDTHSMFQHQ TSVSHQPIVR QCGPQTGIQS
PPSSLQPAEF LYSHGVPRTL SPHQYHSVHG VGMVPEWSEN S
