TBX5_RAT
ID TBX5_RAT Reviewed; 517 AA.
AC Q5I2P1;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=T-box transcription factor TBX5;
DE Short=T-box protein 5;
GN Name=Tbx5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Brown Norway; TISSUE=Heart;
RA Gong L.G., Sun K.L., Qiu G.R., Xu X.Y., Xin N.;
RT "TBX5 cDNA clone from Rattus norvegicus embryo heart.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-binding protein that regulates the transcription of
CC several genes and is involved in heart development and limb pattern
CC formation. Binds to the core DNA motif of NPPA promoter.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SUBUNIT: Monomer. Homodimer (via the T-box); binds DNA as homodimer.
CC Interacts (via the T-box) with NKX2-5 (via the homeobox); this complex
CC binds DNA. Interacts with GATA4. Interacts with KAT2A and KAT2B.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q99593,
CC ECO:0000255|PROSITE-ProRule:PRU00201}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q99593}. Note=Shuttles between the cytoplasm and
CC the nucleus. Acetylation at Lys-338 promotes nuclear retention.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- DOMAIN: The T-Box domain binds to double-stranded DNA.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- PTM: Acetylation at Lys-338 by KAT2A and KAT2B promotes nuclear
CC retention. {ECO:0000250|UniProtKB:Q99593}.
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DR EMBL; AY859491; AAW33688.1; -; mRNA.
DR RefSeq; NP_001009964.1; NM_001009964.1.
DR AlphaFoldDB; Q5I2P1; -.
DR SMR; Q5I2P1; -.
DR STRING; 10116.ENSRNOP00000001893; -.
DR PaxDb; Q5I2P1; -.
DR GeneID; 304514; -.
DR KEGG; rno:304514; -.
DR UCSC; RGD:1305702; rat.
DR CTD; 6910; -.
DR RGD; 1305702; Tbx5.
DR eggNOG; KOG3585; Eukaryota.
DR InParanoid; Q5I2P1; -.
DR PhylomeDB; Q5I2P1; -.
DR PRO; PR:Q5I2P1; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0032993; C:protein-DNA complex; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:RGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0048513; P:animal organ development; ISS:UniProtKB.
DR GO; GO:0003283; P:atrial septum development; ISO:RGD.
DR GO; GO:0060413; P:atrial septum morphogenesis; ISO:RGD.
DR GO; GO:0003167; P:atrioventricular bundle cell differentiation; ISO:RGD.
DR GO; GO:0060928; P:atrioventricular node cell development; ISO:RGD.
DR GO; GO:0060929; P:atrioventricular node cell fate commitment; ISO:RGD.
DR GO; GO:0003181; P:atrioventricular valve morphogenesis; ISO:RGD.
DR GO; GO:0086054; P:bundle of His cell to Purkinje myocyte communication by electrical coupling; ISO:RGD.
DR GO; GO:0003166; P:bundle of His development; ISO:RGD.
DR GO; GO:0003218; P:cardiac left ventricle formation; ISO:RGD.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; ISO:RGD.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; ISS:UniProtKB.
DR GO; GO:0086019; P:cell-cell signaling involved in cardiac conduction; ISO:RGD.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; ISS:UniProtKB.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISS:UniProtKB.
DR GO; GO:0003197; P:endocardial cushion development; ISO:RGD.
DR GO; GO:0035136; P:forelimb morphogenesis; ISO:RGD.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0030324; P:lung development; ISO:RGD.
DR GO; GO:0002009; P:morphogenesis of an epithelium; ISO:RGD.
DR GO; GO:0060044; P:negative regulation of cardiac muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0007389; P:pattern specification process; ISO:RGD.
DR GO; GO:0060039; P:pericardium development; ISS:UniProtKB.
DR GO; GO:1903781; P:positive regulation of cardiac conduction; ISO:RGD.
DR GO; GO:0060045; P:positive regulation of cardiac muscle cell proliferation; ISO:RGD.
DR GO; GO:0051891; P:positive regulation of cardioblast differentiation; ISS:UniProtKB.
DR GO; GO:1903598; P:positive regulation of gap junction assembly; ISO:RGD.
DR GO; GO:0072513; P:positive regulation of secondary heart field cardioblast proliferation; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0003163; P:sinoatrial node development; ISO:RGD.
DR GO; GO:0060290; P:transdifferentiation; IMP:RGD.
DR GO; GO:0003229; P:ventricular cardiac muscle tissue development; ISO:RGD.
DR GO; GO:0003281; P:ventricular septum development; ISO:RGD.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..517
FT /note="T-box transcription factor TBX5"
FT /id="PRO_0000184437"
FT DNA_BIND 58..238
FT /note="T-box"
FT /evidence="ECO:0000250|UniProtKB:Q99593,
FT ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 270..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 270..298
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 331..345
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 348..369
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 338
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q99593"
SQ SEQUENCE 517 AA; 57745 MW; 24F233DE80FAD872 CRC64;
MADADEGFGL ARTPLEPDSK DRSCDSKPES ALGAPSKSPS SPQAAFTQQG MEGIKVFLHE
RELWLKFHEV GTEMIITKAG RQMFPSYKVK VTGLNPKTKY ILLMDIVPAD DHRYKFADNK
WSVTGKAEPA MPGRLYVHPD SPATGAHWMR QLVSFQKLKL TNNHLDPFGH IILNSMHKYQ
PRLHIVKADE NNGFGSKNTA FCTHVFPETA FIAVTSYQNH KITQLKIENN PFAKGFRGSD
DLELHRMSRM RSKEYPVVPR STVRHKVANH SPFSSETRAL STSSNLGSQY QCENGVSGPS
QDLLPPPNPY PLAQEHSQIY HCTKRKDEEC SSTEHPYKKP YMETSPSEED TFYRSGYPQQ
QGLSTSYRTE SAQRQACMYA SSAPPSEPVP SLEDISCNTW PSMPSYSSCT VTTVQPMDRL
PYQHFSAHFT SGPLVPRLAG MANHGSPQLG EGMFQHQTSV THQPVVRQCG PQTGLQSPGS
LQPPEFLYTH GVPRTLSPHQ YHSVHGVGMV PEWSENS
