TBX5_XENLA
ID TBX5_XENLA Reviewed; 519 AA.
AC Q9W7C2; Q9IBC5;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=T-box transcription factor TBX5;
DE Short=T-box protein 5;
GN Name=tbx5;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=10079235; DOI=10.1242/dev.126.8.1739;
RA Horb M.E., Thomsen G.H.;
RT "Tbx5 is essential for heart development.";
RL Development 126:1739-1751(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 118-250.
RX PubMed=10704879; DOI=10.1016/s0925-4773(99)00329-9;
RA Takabatake Y., Takabatake T., Takeshima K.;
RT "Conserved and divergent expression of T-box genes Tbx2-Tbx5 in Xenopus.";
RL Mech. Dev. 91:433-437(2000).
CC -!- FUNCTION: DNA-binding protein that regulates the transcription of
CC several genes and is involved in heart development and limb pattern
CC formation (PubMed:10079235). May bind to the core DNA motif of
CC promoters (By similarity). {ECO:0000250|UniProtKB:Q99593,
CC ECO:0000269|PubMed:10079235}.
CC -!- SUBUNIT: Monomer. Homodimer (via the T-box); binds DNA as homodimer.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q99593,
CC ECO:0000255|PROSITE-ProRule:PRU00201}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q99593}. Note=Shuttles between the cytoplasm and
CC the nucleus. {ECO:0000250|UniProtKB:Q99593}.
CC -!- DEVELOPMENTAL STAGE: Expressed exclusively in the developing heart and
CC eye. Expression begins within a subset of cardioblasts at neurula
CC stages and continues within the heart during morphogenesis at later
CC tadpole stages. Within the heart tube, expressed in all but the most
CC anterior domain, the bulbus cordis. {ECO:0000269|PubMed:10079235}.
CC -!- DOMAIN: The T-Box domain binds to double-stranded DNA.
CC {ECO:0000250|UniProtKB:Q99593}.
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DR EMBL; AF133036; AAD23592.1; -; mRNA.
DR EMBL; AB032944; BAA93084.1; -; mRNA.
DR RefSeq; NP_001079170.1; NM_001085701.2.
DR AlphaFoldDB; Q9W7C2; -.
DR SMR; Q9W7C2; -.
DR GeneID; 373738; -.
DR KEGG; xla:373738; -.
DR CTD; 373738; -.
DR Xenbase; XB-GENE-865653; tbx5.L.
DR Proteomes; UP000186698; Chromosome 1L.
DR Bgee; 373738; Expressed in heart and 3 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0032993; C:protein-DNA complex; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0048513; P:animal organ development; ISS:UniProtKB.
DR GO; GO:0007267; P:cell-cell signaling; ISS:UniProtKB.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; ISS:UniProtKB.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISS:UniProtKB.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0060044; P:negative regulation of cardiac muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0060039; P:pericardium development; ISS:UniProtKB.
DR GO; GO:0051891; P:positive regulation of cardioblast differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..519
FT /note="T-box transcription factor TBX5"
FT /id="PRO_0000262469"
FT DNA_BIND 63..238
FT /note="T-box"
FT /evidence="ECO:0000250|UniProtKB:Q99593,
FT ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 254..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 262..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 326..346
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 146
FT /note="A -> T (in Ref. 2; BAA93084)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="D -> A (in Ref. 2; BAA93084)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 519 AA; 58081 MW; 8B5E096C67FC3D1E CRC64;
MADTEEAYGM PDTPVEAEPK ELQCEPKQDN QLGASSKTPT SPPAAFTQQG MEGIKVFLHE
RELWLKFHEV GTEMIITKAG RRMFPSYKVK VTGLNPKTKY ILLMDIVPAD DHRYKFADNK
WSVTGKAEPA MPGRLYVHPD SPATGAHWMR QLVSFQKLKL TNNHLDPFGH IILNSMHKYQ
PRLHIVKADE NNGFGSKNTA FCTHVFSETD FIAVTSYQNH KITQLKIENN PFAKGFRGSD
DMELHRMSRM QSKEYPVVPR STVRQKVSSN HSPFSQETRN ITGSSTLNSQ YQCENGVSST
SQDLLPSSSA YTSLPHESGT IYHCTKRKVS EEPAEHSYKK PYMDTSPSEE DPFYRSGYPQ
PSSSSSSTTS FRTESAQRQA CMYASSAPAT EPVPSIEDIS CNSWSSVPSY SSCTVGGGMQ
PMERLPYQHF SAHFTSSSLM PRLSNHAGTQ PSDSHSMFQH QSSHQAIVRQ CNPQSGLQQS
SALQPTEFLY PHSVPRTISP HQYHSVHGVG MVPDWNENS
