TBX5_XENTR
ID TBX5_XENTR Reviewed; 519 AA.
AC Q3SA47;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=T-box transcription factor TBX5;
DE Short=T-box protein 5;
GN Name=tbx5;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=16477648; DOI=10.1002/dvdy.20714;
RA Showell C., Christine K.S., Mandel E.M., Conlon F.L.;
RT "Developmental expression patterns of Tb x 1, Tb x 2, Tb x 5, and Tb x 20
RT in Xenopus tropicalis.";
RL Dev. Dyn. 235:1623-1630(2006).
CC -!- FUNCTION: DNA-binding protein that regulates the transcription of
CC several genes and is involved in heart development and limb pattern
CC formation. May bind to the core DNA motif of promoters.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SUBUNIT: Monomer. Homodimer (via the T-box); binds DNA as homodimer.
CC {ECO:0000250|UniProtKB:Q99593}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q99593,
CC ECO:0000255|PROSITE-ProRule:PRU00201}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q99593}. Note=Shuttles between the cytoplasm and
CC the nucleus. {ECO:0000250|UniProtKB:Q99593}.
CC -!- DEVELOPMENTAL STAGE: In late neurulae (stage 19), expression seen
CC within the eye anlagen, with higher levels dorsally. At early tail bud
CC stage (stage 25), expressed in each developing eye and in the heart
CC primordia, located ventrally. In stage 26 embryos, the Tbx5-expressing
CC cells of the heart primordia are seen to converge at the ventral
CC midline, while expression also seen in two bilateral groups of cells
CC continuous with and extending dorsally from the heart primordia.
CC Expression in the dorsal region of the eye found to be maintained until
CC early tadpole stages (stage 40), although expression becomes greatly
CC restricted between stages 33 and 40. At stage 31/32, strong expression
CC detected in the posterior region of the heart tube. After looping of
CC the heart, a higher level of expression detected in the ventricle than
CC in the atrium. {ECO:0000269|PubMed:16477648}.
CC -!- DOMAIN: The T-Box domain binds to double-stranded DNA.
CC {ECO:0000250|UniProtKB:Q99593}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ124207; AAZ79652.1; -; mRNA.
DR AlphaFoldDB; Q3SA47; -.
DR SMR; Q3SA47; -.
DR STRING; 8364.ENSXETP00000059243; -.
DR eggNOG; KOG3585; Eukaryota.
DR InParanoid; Q3SA47; -.
DR Proteomes; UP000008143; Genome assembly.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0032993; C:protein-DNA complex; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0048513; P:animal organ development; ISS:UniProtKB.
DR GO; GO:0003218; P:cardiac left ventricle formation; IBA:GO_Central.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; ISS:UniProtKB.
DR GO; GO:0035115; P:embryonic forelimb morphogenesis; ISS:UniProtKB.
DR GO; GO:0030326; P:embryonic limb morphogenesis; ISS:UniProtKB.
DR GO; GO:0007507; P:heart development; ISS:UniProtKB.
DR GO; GO:0060044; P:negative regulation of cardiac muscle cell proliferation; ISS:UniProtKB.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0007389; P:pattern specification process; IBA:GO_Central.
DR GO; GO:0060039; P:pericardium development; ISS:UniProtKB.
DR GO; GO:0051891; P:positive regulation of cardioblast differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..519
FT /note="T-box transcription factor TBX5"
FT /id="PRO_0000262470"
FT DNA_BIND 63..238
FT /note="T-box"
FT /evidence="ECO:0000250|UniProtKB:Q99593,
FT ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 254..312
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 335..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 262..308
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..376
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 519 AA; 58104 MW; 408F01226E45232B CRC64;
MADTEEAYGM PDTPVEAEPK ELQCEPKQDN QMGASSKTPT SPQAAFTQQG MEGIKVFLHE
RELWLKFHEV GTEMIITKAG RRMFPSYKVK VTGLNPKTKY ILLMDIVPAD DHRYKFADNK
WSVTGKAEPA MPGRLYVHPD SPATGTHWMR QLVSSQKLKL TNNHLDPFGH IILNSMHKYQ
PRLHIVKADE NNGFGSKNTA FCTHVFSETA FIAATSYQNH KITQLKIENN PFAKGFRGSD
DMELHRMSRM QSKEYPVVPR STVRQKVSSN HSPFSQETRN ITGSSTLNSQ YQCENGVSST
SQDLLPPSSA YPSLPHESAP IYHCTKRKVS EEPAELSYKK PYMDTSPSEE DPYYRSGYPQ
PSSSSSSNNS FRTESAQRQA CMYASSAPAT EPVPSLEDIS CNSWSSVPPY SSCTVGGGMQ
PMERLPYQHF SAHFTSSSLM PRLSNHGSTQ PSDSHSMFQH QSTHQTIVRQ CNPQPGLQQS
SALQSTEFLY PHSVPRTISP HQYHSVHGVG MAPDWNENS
