TBX8_CAEEL
ID TBX8_CAEEL Reviewed; 315 AA.
AC Q22292; Q75WK3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 3.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=T-box transcription factor tbx-8;
GN Name=tbx-8; ORFNames=T07C4.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2; TISSUE=Embryo;
RX PubMed=15066124; DOI=10.1111/j.1356-9597.2004.00725.x;
RA Andachi Y.;
RT "Caenorhabditis elegans T-box genes tbx-9 and tbx-8 are required for
RT formation of hypodermis and body-wall muscle in embryogenesis.";
RL Genes Cells 9:331-344(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15102704; DOI=10.1242/dev.01110;
RA Pocock R., Ahringer J., Mitsch M., Maxwell S., Woollard A.;
RT "A regulatory network of T-box genes and the even-skipped homologue vab-7
RT controls patterning and morphogenesis in C. elegans.";
RL Development 131:2373-2385(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=15892873; DOI=10.1186/gb-2005-6-5-r45;
RA Baugh L.R., Wen J.C., Hill A.A., Slonim D.K., Brown E.L., Hunter C.P.;
RT "Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic
RT patterning genes identifies conserved genetic interactions.";
RL Genome Biol. 6:RESEARCH0045.1-RESEARCH0045.8(2005).
CC -!- FUNCTION: Transcription factor (By similarity). Involved in the control
CC of early morphogenesis of the intestine, hypodermis and body-wall
CC muscle (PubMed:15066124, PubMed:15102704). Involved in regulating
CC expression of vab-7 (PubMed:15102704). Appears to have partially
CC redundant function to tbx-9 (PubMed:15066124).
CC {ECO:0000250|UniProtKB:Q16650, ECO:0000269|PubMed:15066124,
CC ECO:0000269|PubMed:15102704}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251,
CC ECO:0000269|PubMed:15066124, ECO:0000269|PubMed:15102704}.
CC -!- DEVELOPMENTAL STAGE: Expressed during morphogenesis in the hypodermis,
CC body-wall muscle, intestine and dorsal, lateral and ventral hypodermal
CC cells (PubMed:15066124, PubMed:15102704). First detected in the Ea and
CC Ep cells until the 24 cell stage (PubMed:15066124, PubMed:15102704). At
CC the 24 cell stage detected in 5 cells including AB descendant cells and
CC also the Ca and Cp cells (PubMed:15066124). At the 200 cell stage,
CC detected in two pairs of MS descendant cells (PubMed:15066124).
CC Thereafter detected in a subset of cells at each stage up to
CC approximately the 400 cell stage, becoming undetectable just before
CC morphogenesis (PubMed:15066124). During morphogenesis detected in the
CC hypodermis, body-wall muscle, intestine and dorsal, lateral and ventral
CC hypodermal cells up until early L1 larval stage (PubMed:15066124,
CC PubMed:15102704). {ECO:0000269|PubMed:15066124,
CC ECO:0000269|PubMed:15102704}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes about 5% embryonic
CC lethality with 1-10% of hatching larvae displaying posterior
CC morphological defects (PubMed:15102704, PubMed:15892873). Defective
CC intercalation of dorsal hypodermis (PubMed:15102704). Simultaneous
CC RNAi-mediated knockdown of tbx-9 causes at least 48% of embryos to fail
CC to hatch and those that do display severe morphological defects and die
CC as larvae (PubMed:15892873, PubMed:15102704). Simultaneous RNAi-
CC mediated knockdown of tbx-9 abolishes muscle expression of vab-7
CC (PubMed:15102704). {ECO:0000269|PubMed:15102704,
CC ECO:0000269|PubMed:15892873}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB121091; BAD14918.1; -; mRNA.
DR EMBL; Z29443; CAA82578.2; -; Genomic_DNA.
DR PIR; C88579; C88579.
DR RefSeq; NP_499287.2; NM_066886.3.
DR AlphaFoldDB; Q22292; -.
DR SMR; Q22292; -.
DR BioGRID; 41643; 23.
DR IntAct; Q22292; 23.
DR STRING; 6239.T07C4.2; -.
DR EPD; Q22292; -.
DR PaxDb; Q22292; -.
DR EnsemblMetazoa; T07C4.2.1; T07C4.2.1; WBGene00006545.
DR EnsemblMetazoa; T07C4.2.2; T07C4.2.2; WBGene00006545.
DR GeneID; 176450; -.
DR KEGG; cel:CELE_T07C4.2; -.
DR UCSC; T07C4.2; c. elegans.
DR CTD; 176450; -.
DR WormBase; T07C4.2; CE36891; WBGene00006545; tbx-8.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00970000196046; -.
DR HOGENOM; CLU_032588_1_0_1; -.
DR InParanoid; Q22292; -.
DR OMA; PRRLMLH; -.
DR OrthoDB; 1306558at2759; -.
DR PhylomeDB; Q22292; -.
DR Reactome; R-CEL-2032785; YAP1- and WWTR1 (TAZ)-stimulated gene expression.
DR SignaLink; Q22292; -.
DR PRO; PR:Q22292; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00006545; Expressed in embryo and 18 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; ISS:WormBase.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0010172; P:embryonic body morphogenesis; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:WormBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..315
FT /note="T-box transcription factor tbx-8"
FT /id="PRO_0000184471"
FT DNA_BIND 11..195
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 193..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 195..209
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..231
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 315 AA; 35910 MW; 9163326BEFCDC2F5 CRC64;
MTDQVYVTLR EDQDKLWNLF HYHKNEMIVT KSGRKMFPKL EYVVRGLTPN KLYAMMLHIE
PSDDHRYKFS SGEWVKSGKA EKHREPKKLW HADGVRSGKE WMTNPVCFDR VKITNCAEST
NASMIFLHSM HKYTPVMSIY ESPSESPFSV PQPSTRLVTS VRLTYTEFIA VTAYQNDAVI
KLKIKFNPFA KGFREGSQSD RKRNSPSADD STTDESSSQV SSPQPKKSRT VSVSPPLMPR
ILPTMIPPPP AINPLFYSLP YFSHLAATGN FPPVPFQFPF GLPCFSPMSF PTPPPSLKNV
KKEEQEDIEQ EINVV
