TBX9_CAEEL
ID TBX9_CAEEL Reviewed; 292 AA.
AC Q22289; Q86M50;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=T-box transcription factor tbx-9;
GN Name=tbx-9; ORFNames=T07C4.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2;
RX PubMed=15066124; DOI=10.1111/j.1356-9597.2004.00725.x;
RA Andachi Y.;
RT "Caenorhabditis elegans T-box genes tbx-9 and tbx-8 are required for
RT formation of hypodermis and body-wall muscle in embryogenesis.";
RL Genes Cells 9:331-344(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15102704; DOI=10.1242/dev.01110;
RA Pocock R., Ahringer J., Mitsch M., Maxwell S., Woollard A.;
RT "A regulatory network of T-box genes and the even-skipped homologue vab-7
RT controls patterning and morphogenesis in C. elegans.";
RL Development 131:2373-2385(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=15892873; DOI=10.1186/gb-2005-6-5-r45;
RA Baugh L.R., Wen J.C., Hill A.A., Slonim D.K., Brown E.L., Hunter C.P.;
RT "Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic
RT patterning genes identifies conserved genetic interactions.";
RL Genome Biol. 6:RESEARCH0045.1-RESEARCH0045.8(2005).
RN [5] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24885717; DOI=10.1186/1471-213x-14-17;
RA Liu W.J., Reece-Hoyes J.S., Walhout A.J., Eisenmann D.M.;
RT "Multiple transcription factors directly regulate Hox gene lin-39
RT expression in ventral hypodermal cells of the C. elegans embryo and larva,
RT including the hypodermal fate regulators LIN-26 and ELT-6.";
RL BMC Dev. Biol. 14:17-17(2014).
CC -!- FUNCTION: Transcription factor (By similarity). Involved in the control
CC of early morphogenesis of the intestine, hypodermis and body-wall
CC muscle (PubMed:15066124, PubMed:15102704). Involved in regulating
CC expression of vab-7 (PubMed:15102704). Appears to have partially
CC redundant function to tbx-8 (PubMed:15066124). Positively modulates
CC expression of homeobox protein lin-39, perhaps by binding to regulatory
CC regions of the lin-39 gene, acting in the vulval lineage.
CC {ECO:0000250|UniProtKB:Q16650, ECO:0000269|PubMed:15066124,
CC ECO:0000269|PubMed:15102704, ECO:0000269|PubMed:24885717}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251,
CC ECO:0000269|PubMed:15066124, ECO:0000269|PubMed:15102704}.
CC -!- DEVELOPMENTAL STAGE: Expressed during morphogenesis in the hypodermis,
CC body-wall muscle, intestine and dorsal, lateral and ventral hypodermal
CC cells (PubMed:15066124, PubMed:15102704). First detected in the E cell
CC at the 8 cell stage and its daughter Ea and Ep cells until the 24 cell
CC stage (PubMed:15066124, PubMed:15102704). At the 24 cell stage detected
CC in 5 cells including AB descendant cells and also the Ca and Cp cells
CC (PubMed:15066124). At the 200 cell stage, detected in two pairs of MS
CC descendant cells (PubMed:15066124). Thereafter detected in a subset of
CC cells at each stage up to approximately the 400 cell stage, becoming
CC undetectable just before morphogenesis (PubMed:15066124). During
CC morphogenesis detected in the hypodermis, body-wall muscle, intestine
CC and dorsal, lateral and ventral hypodermal cells up until early L1
CC larval stage (PubMed:15066124, PubMed:15102704).
CC {ECO:0000269|PubMed:15066124, ECO:0000269|PubMed:15102704}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes about 5% embryonic
CC lethality with 1-10% of hatching larvae displaying posterior
CC morphological defects (PubMed:15892873, PubMed:15102704). Defective
CC intercalation of dorsal hypodermis (PubMed:15102704). Simultaneous
CC RNAi-mediated knockdown of tbx-8 causes at least 48% of embryos to fail
CC to hatch and those that do display severe morphological defects and die
CC as larvae (PubMed:15892873, PubMed:15102704). Simultaneous RNAi-
CC mediated knockdown of tbx-8 abolishes muscle expression of vab-7
CC (PubMed:15102704). RNAi-mediated knockdown at the larval L1 stage
CC causes a decrease in expression of lin-39 at the larval L3 stage
CC (PubMed:24885717). Knockdown in L1 stage larvae, in a lin-39 mutant
CC background, causes abnormal fusion of vulval precursor cells at larval
CC stage L2 (PubMed:24885717). {ECO:0000269|PubMed:15102704,
CC ECO:0000269|PubMed:15892873, ECO:0000269|PubMed:24885717}.
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DR EMBL; AB019521; BAC66463.1; -; mRNA.
DR EMBL; Z29443; CAA82575.2; -; Genomic_DNA.
DR PIR; S41019; S41019.
DR RefSeq; NP_499286.2; NM_066885.4.
DR AlphaFoldDB; Q22289; -.
DR SMR; Q22289; -.
DR BioGRID; 41642; 122.
DR IntAct; Q22289; 118.
DR STRING; 6239.T07C4.6; -.
DR MoonDB; Q22289; Predicted.
DR PaxDb; Q22289; -.
DR EnsemblMetazoa; T07C4.6.1; T07C4.6.1; WBGene00006546.
DR GeneID; 176449; -.
DR KEGG; cel:CELE_T07C4.6; -.
DR UCSC; T07C4.6; c. elegans.
DR CTD; 176449; -.
DR WormBase; T07C4.6; CE36892; WBGene00006546; tbx-9.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00970000196046; -.
DR HOGENOM; CLU_032588_0_0_1; -.
DR InParanoid; Q22289; -.
DR OMA; MIPLHSM; -.
DR OrthoDB; 1306558at2759; -.
DR PhylomeDB; Q22289; -.
DR Reactome; R-CEL-2032785; YAP1- and WWTR1 (TAZ)-stimulated gene expression.
DR SignaLink; Q22289; -.
DR PRO; PR:Q22289; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00006546; Expressed in embryo and 18 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; ISS:WormBase.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0010172; P:embryonic body morphogenesis; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:WormBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..292
FT /note="T-box transcription factor tbx-9"
FT /id="PRO_0000184472"
FT DNA_BIND 10..194
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 192..227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 292 AA; 33045 MW; CE1424246959C787 CRC64;
MSKVKVSIEG SQETLWKIFH AEVNEMIVTK NGRKLFPKLE YIVEGLDENK LYAIMLQLQP
VGESRFKFSG GKWQETGKAE KQVDAKKMWH ADGVRKGSDW MWSSICFDRV KISNYSESNN
ASMIYLNSMH KYIPVLTIYE SPSESPFCVP QSSNQIVATA KFPHTEFIAV TAYQNQKITD
LKIKHNSFAK GFRDGNLSRK RRSPSYSDGS NSQSPSPKSR SPPEVAPLQS MPPINPFLFY
FPHMLSENLP VQFPFAFPFL SPLPSTPSSS SSELSIVKEE DQEVEEDIDI VG
