TBXTA_DANRE
ID TBXTA_DANRE Reviewed; 423 AA.
AC Q07998; B3DG73;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=T-box transcription factor T-A {ECO:0000305};
DE AltName: Full=Brachyury protein homolog-A {ECO:0000305};
DE AltName: Full=No tail protein A-A {ECO:0000305};
DE Short=No tail protein-A;
DE AltName: Full=Protein T homolog-A {ECO:0000305};
DE AltName: Full=T-box protein ZfT-A {ECO:0000305};
DE Short=Zf-T-A;
GN Name=tbxta; Synonyms=ntl, ntla, t;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RC TISSUE=Embryo;
RX PubMed=1295726; DOI=10.1242/dev.116.4.1021;
RA Schulte-Merker S., Ho R.K., Herrmann B.G., Nuesslein-Volhard C.;
RT "The protein product of the zebrafish homologue of the mouse T gene is
RT expressed in nuclei of the germ ring and the notochord of the early
RT embryo.";
RL Development 116:1021-1032(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AB;
RX PubMed=15933901; DOI=10.1007/s10126-004-3500-8;
RA Yamakoshi K., Shishido Y., Shimoda N.;
RT "Generation of aberrant transcripts of and free DNA ends in zebrafish no
RT tail gene.";
RL Mar. Biotechnol. 7:163-172(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=8402905; DOI=10.1016/s0092-8674(05)80087-x;
RA Halpern M.E., Ho R.K., Walker C., Kimmel C.B.;
RT "Induction of muscle pioneers and floor plate is distinguished by the
RT zebrafish no tail mutation.";
RL Cell 75:99-111(1993).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=7600961; DOI=10.1242/dev.120.4.843;
RA Schulte-Merker S., Hammerschmidt M., Beuchle D., Cho K.W.,
RA De Robertis E.M., Nuesslein-Volhard C.;
RT "Expression of zebrafish goosecoid and no tail gene products in wild-type
RT and mutant no tail embryos.";
RL Development 120:843-852(1994).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=7600949; DOI=10.1242/dev.120.4.1009;
RA Schulte-Merker S., van Eeden F.J.M., Halpern M.E., Kimmel C.B.,
RA Nuesslein-Volhard C.;
RT "no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury)
RT gene.";
RL Development 120:1009-1015(1994).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12091302; DOI=10.1242/dev.129.14.3311;
RA Amacher S.L., Draper B.W., Summers B.R., Kimmel C.B.;
RT "The zebrafish T-box genes no tail and spadetail are required for
RT development of trunk and tail mesoderm and medial floor plate.";
RL Development 129:3311-3323(2002).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15084283; DOI=10.1016/j.cub.2004.04.002;
RA Amack J.D., Yost H.J.;
RT "The T box transcription factor no tail in ciliated cells controls
RT zebrafish left-right asymmetry.";
RL Curr. Biol. 14:685-690(2004).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=14660439; DOI=10.1242/dev.00915;
RA Marlow F., Gonzalez E.M., Yin C., Rojo C., Solnica-Krezel L.;
RT "No tail co-operates with non-canonical Wnt signaling to regulate posterior
RT body morphogenesis in zebrafish.";
RL Development 131:203-216(2004).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17717538; DOI=10.1038/nchembio.2007.30;
RA Shestopalov I.A., Sinha S., Chen J.K.;
RT "Light-controlled gene silencing in zebrafish embryos.";
RL Nat. Chem. Biol. 3:650-651(2007).
RN [11]
RP DISRUPTION PHENOTYPE.
RX PubMed=17294249; DOI=10.1007/s11033-007-9063-9;
RA Pei D.-S., Sun Y.-H., Long Y., Zhu Z.-Y.;
RT "Inhibition of no tail (ntl) gene expression in zebrafish by external guide
RT sequence (EGS) technique.";
RL Mol. Biol. Rep. 35:139-143(2008).
CC -!- FUNCTION: Involved in the transcriptional regulation of genes required
CC for mesoderm differentiation, including itself. Indispensable for the
CC formation of the notochord and the tail structure. Functions together
CC with tbx16/spadetail in development of trunk and tail mesoderm.
CC Functions by itself early in development to repress medial floor plate
CC and promote notochord fate but at later times, functions together with
CC tbx16/spadetail to promote medial floor plate formation. Acts in a
CC parallel pathway to, but cooperates with, non-canonical wnt-signaling
CC during tail formation. Required for the morphogenesis of Kupffer's
CC vesicle and regulates left-right asymmetry.
CC {ECO:0000269|PubMed:12091302, ECO:0000269|PubMed:14660439,
CC ECO:0000269|PubMed:15084283, ECO:0000269|PubMed:17717538,
CC ECO:0000269|PubMed:7600949}.
CC -!- SUBUNIT: Monomer. Binds DNA as a monomer.
CC {ECO:0000250|UniProtKB:P20293}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201,
CC ECO:0000269|PubMed:1295726, ECO:0000269|PubMed:7600949}.
CC -!- TISSUE SPECIFICITY: First expressed at the dorsal side of the blastula
CC embryo. Expressed in the germ ring, shield and chordamesoderm during
CC gastrulation and is restricted to the notochord and tailbud during
CC somitogenesis (at protein level). {ECO:0000269|PubMed:12091302,
CC ECO:0000269|PubMed:1295726, ECO:0000269|PubMed:17717538,
CC ECO:0000269|PubMed:7600961}.
CC -!- DEVELOPMENTAL STAGE: In the embryo, first expressed at the doming stage
CC (4.2 hours). Highest expression is found between 5.2 and 9.5 hours
CC (epiboly) after which levels gradually decrease, with expression
CC becoming absent by 48 hours. {ECO:0000269|PubMed:1295726}.
CC -!- INDUCTION: By mesoderm-inducing factor activin A.
CC {ECO:0000269|PubMed:1295726}.
CC -!- DISRUPTION PHENOTYPE: Loss of notochord cells and posterior structures.
CC Disrupts left-right development. {ECO:0000269|PubMed:14660439,
CC ECO:0000269|PubMed:15084283, ECO:0000269|PubMed:17294249,
CC ECO:0000269|PubMed:17717538, ECO:0000269|PubMed:8402905}.
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DR EMBL; S57147; AAB25829.1; -; mRNA.
DR EMBL; AB088068; BAC79149.1; -; Genomic_DNA.
DR EMBL; BC162297; AAI62297.1; -; mRNA.
DR EMBL; BC162300; AAI62300.1; -; mRNA.
DR PIR; A49125; A49125.
DR RefSeq; NP_571237.1; NM_131162.1.
DR AlphaFoldDB; Q07998; -.
DR SMR; Q07998; -.
DR BioGRID; 78591; 4.
DR STRING; 7955.ENSDARP00000005351; -.
DR PaxDb; Q07998; -.
DR Ensembl; ENSDART00000164594; ENSDARP00000141934; ENSDARG00000101576.
DR GeneID; 30399; -.
DR KEGG; dre:30399; -.
DR CTD; 30399; -.
DR ZFIN; ZDB-GENE-980526-437; tbxta.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00940000166269; -.
DR HOGENOM; CLU_038303_1_1_1; -.
DR InParanoid; Q07998; -.
DR OMA; PAHDSWS; -.
DR OrthoDB; 1201455at2759; -.
DR PhylomeDB; Q07998; -.
DR TreeFam; TF106341; -.
DR PRO; PR:Q07998; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 19.
DR Bgee; ENSDARG00000101576; Expressed in cranial skeletal system and 39 other tissues.
DR GO; GO:0000785; C:chromatin; IDA:ZFIN.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:ZFIN.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:ZFIN.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:ZFIN.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0060028; P:convergent extension involved in axis elongation; IGI:ZFIN.
DR GO; GO:0072045; P:convergent extension involved in nephron morphogenesis; IMP:ZFIN.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; IMP:ZFIN.
DR GO; GO:0035462; P:determination of left/right asymmetry in diencephalon; IMP:ZFIN.
DR GO; GO:0003140; P:determination of left/right asymmetry in lateral mesoderm; IMP:ZFIN.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR GO; GO:0048565; P:digestive tract development; IGI:ZFIN.
DR GO; GO:0003143; P:embryonic heart tube morphogenesis; IMP:ZFIN.
DR GO; GO:0001947; P:heart looping; IMP:ZFIN.
DR GO; GO:0003007; P:heart morphogenesis; IBA:GO_Central.
DR GO; GO:0070121; P:Kupffer's vesicle development; IMP:ZFIN.
DR GO; GO:0001889; P:liver development; IGI:ZFIN.
DR GO; GO:0007498; P:mesoderm development; IMP:ZFIN.
DR GO; GO:0001707; P:mesoderm formation; IGI:ZFIN.
DR GO; GO:0060034; P:notochord cell differentiation; IMP:ZFIN.
DR GO; GO:0030903; P:notochord development; IMP:ZFIN.
DR GO; GO:0014028; P:notochord formation; IMP:ZFIN.
DR GO; GO:0031016; P:pancreas development; IGI:ZFIN.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0036342; P:post-anal tail morphogenesis; IMP:ZFIN.
DR GO; GO:0035775; P:pronephric glomerulus morphogenesis; IMP:ZFIN.
DR GO; GO:2000223; P:regulation of BMP signaling pathway involved in heart jogging; IMP:ZFIN.
DR GO; GO:0042663; P:regulation of endodermal cell fate specification; IGI:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0061053; P:somite development; IMP:ZFIN.
DR GO; GO:0001756; P:somitogenesis; IMP:ZFIN.
DR GO; GO:0010159; P:specification of animal organ position; IMP:ZFIN.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR002070; TF_Brachyury.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00938; BRACHYURY.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..423
FT /note="T-box transcription factor T-A"
FT /id="PRO_0000184418"
FT DNA_BIND 44..212
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 215..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 280..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 423 AA; 45834 MW; 96FEB8109EF4407E CRC64;
MSASSPDQRL DHLLSAVESE FQKGSEKGDA SERDIKLSLE DAELWTKFKE LTNEMIVTKT
GRRMFPVLRA SVTGLDPNAM YSVLLDFVAA DNNRWKYVNG EWVPGGKPEP QSPSCVYIHP
DSPNFGAHWM KAPVSFSKVK LSNKLNGGGQ IMLNSLHKYE PRIHIVKVGG IQKMISSQSF
PETQFIAVTA YQNEEITALK IKHNPFAKAF LDAKERSDHK EVPDHSTDNQ QSGYSQLGGW
FLPSNGPMGP SSSPPQFNGA PVHSSGSYCE RYSSLRNHRA APYPSHYSHR STTTNNYMDN
SSGSLASHDS WSALQIPNSS GMGTLAHTTN TTSNTSQYPS LWSVAGTTLT PSGSASGSIT
GGLTSQFLRG SSMSYSGLTS SLPVSSPSSM YDPGLSEVGV GDAQFESSIA RLTASWAPVA
QSY
