ID   TBXT_CANLF              Reviewed;         435 AA.
AC   Q9GL27;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=T-box transcription factor T {ECO:0000305};
DE   AltName: Full=Brachyury protein {ECO:0000305};
DE   AltName: Full=Protein T;
GN   Name=TBXT {ECO:0000250|UniProtKB:O15178}; Synonyms=T;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-63, AND POLYMORPHISM.
RX   PubMed=11252170; DOI=10.1007/s003350010253;
RA   Haworth K.E., Putt W., Cattanach B., Breen M., Binns M., Lingaas F.,
RA   Edwards Y.H.;
RT   "Canine homolog of the T-box transcription factor T; failure of the protein
RT   to bind to its DNA target leads to a short-tail phenotype.";
RL   Mamm. Genome 12:212-218(2001).
CC   -!- FUNCTION: Involved in the transcriptional regulation of genes required
CC       for mesoderm formation and differentiation. Binds to a palindromic site
CC       (called T site) and activates gene transcription when bound to such a
CC       site. {ECO:0000250, ECO:0000250|UniProtKB:P20293}.
CC   -!- SUBUNIT: Monomer. Binds DNA as a monomer. {ECO:0000250,
CC       ECO:0000250|UniProtKB:P20293}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O15178}.
CC   -!- POLYMORPHISM: A mutation in position 63 is responsible for a short-tail
CC       trait in a bob-tailed dog (PubMed:11252170). The homozygous mutation is
CC       lethal (PubMed:11252170). {ECO:0000269|PubMed:11252170}.
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DR   EMBL; AJ245513; CAC10530.1; -; mRNA.
DR   RefSeq; NP_001003092.1; NM_001003092.1.
DR   AlphaFoldDB; Q9GL27; -.
DR   SMR; Q9GL27; -.
DR   STRING; 9615.ENSCAFP00000050704; -.
DR   PaxDb; Q9GL27; -.
DR   GeneID; 403653; -.
DR   KEGG; cfa:403653; -.
DR   CTD; 6862; -.
DR   eggNOG; KOG3585; Eukaryota.
DR   HOGENOM; CLU_038303_1_1_1; -.
DR   InParanoid; Q9GL27; -.
DR   OMA; RNDHKDM; -.
DR   OrthoDB; 1201455at2759; -.
DR   TreeFam; TF106341; -.
DR   Proteomes; UP000002254; Chromosome 1.
DR   Bgee; ENSCAFG00000000807; Expressed in renal medulla and 10 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR   GO; GO:0003007; P:heart morphogenesis; IBA:GO_Central.
DR   GO; GO:0001707; P:mesoderm formation; IBA:GO_Central.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0001756; P:somitogenesis; IBA:GO_Central.
DR   CDD; cd00182; TBOX; 1.
DR   Gene3D; 2.60.40.820; -; 1.
DR   InterPro; IPR008967; p53-like_TF_DNA-bd.
DR   InterPro; IPR046360; T-box_DNA-bd.
DR   InterPro; IPR036960; T-box_sf.
DR   InterPro; IPR002070; TF_Brachyury.
DR   InterPro; IPR001699; TF_T-box.
DR   InterPro; IPR018186; TF_T-box_CS.
DR   PANTHER; PTHR11267; PTHR11267; 1.
DR   Pfam; PF00907; T-box; 1.
DR   PRINTS; PR00938; BRACHYURY.
DR   PRINTS; PR00937; TBOX.
DR   SMART; SM00425; TBOX; 1.
DR   SUPFAM; SSF49417; SSF49417; 1.
DR   PROSITE; PS01283; TBOX_1; 1.
DR   PROSITE; PS01264; TBOX_2; 1.
DR   PROSITE; PS50252; TBOX_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..435
FT                   /note="T-box transcription factor T"
FT                   /id="PRO_0000184413"
FT   DNA_BIND        51..219
FT                   /note="T-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT   REGION          280..310
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          384..412
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        293..310
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         63
FT                   /note="I -> M (in a bob-tailed dog; alters the ability of
FT                   the protein to bind to its consensus DNA target)"
FT                   /evidence="ECO:0000269|PubMed:11252170"
SQ   SEQUENCE   435 AA;  47148 MW;  CDCDB4A53A395AE2 CRC64;
     MSSPGAESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEDSE LWLRFKELTN
     EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH RWKYVNGEWV PGGKPEPQAP
     SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGAQR
     MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERSDHKDMM EEPGDSQQSG
     YSQWGWLIPG TSTLCPPATP HPQFGGPLSL PSTHGCERYP ALRNHRPSPY PSPYAHRNNS
     PTYSDNPSAC LSMLQSHDSW PSLGTPAHTS MLPMSHSAGP PTGSSQYPSL WSVSNGTITP
     GAQPAGMSNG LGAQFFRGSS AHGAPLGHAV PAPSASGSPL YEGAPTATDV PDSQYDASAQ
     ARLIASWTPV SPPSM