TBXT_MOUSE
ID TBXT_MOUSE Reviewed; 436 AA.
AC P20293;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=T-box transcription factor T {ECO:0000305};
DE AltName: Full=Brachyury protein {ECO:0000305};
DE AltName: Full=Protein T;
GN Name=Tbxt {ECO:0000250|UniProtKB:O15178};
GN Synonyms=T {ECO:0000303|PubMed:2154694};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2154694; DOI=10.1038/343617a0;
RA Herrmann B.G., Labeit S., Poustka A., King T.R., Lehrach H.;
RT "Cloning of the T gene required in mesoderm formation in the mouse.";
RL Nature 343:617-622(1990).
RN [2]
RP DNA-BINDING, AND SUBUNIT.
RX PubMed=8344258; DOI=10.1002/j.1460-2075.1993.tb05990.x;
RA Kispert A., Herrmann B.G.;
RT "The Brachyury gene encodes a novel DNA binding protein.";
RL EMBO J. 12:3211-3220(1993).
RN [3]
RP FUNCTION, DNA-BINDING, AND SUBCELLULAR LOCATION.
RX PubMed=7588606; DOI=10.1002/j.1460-2075.1995.tb00158.x;
RA Kispert A., Koschorz B., Herrmann B.G.;
RT "The T protein encoded by Brachyury is a tissue-specific transcription
RT factor.";
RL EMBO J. 14:4763-4772(1995).
CC -!- FUNCTION: Involved in the transcriptional regulation of genes required
CC for mesoderm formation and differentiation (PubMed:7588606). Binds to a
CC palindromic T site 5'-TTCACACCTAGGTGTGAA-3' DNA sequence and activates
CC gene transcription when bound to such a site (PubMed:8344258,
CC PubMed:7588606). {ECO:0000269|PubMed:7588606,
CC ECO:0000269|PubMed:8344258}.
CC -!- SUBUNIT: Monomer. Binds DNA as a monomer. {ECO:0000269|PubMed:8344258}.
CC -!- INTERACTION:
CC P20293; Q9WUI0: Mixl1; NbExp=7; IntAct=EBI-13637160, EBI-13637214;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:7588606}.
CC -!- DEVELOPMENTAL STAGE: During gastrula and neurula stages in involuting
CC mesoderm and in the notochord.
CC -!- DISEASE: Note=Embryo lacking the T gene fail to form the notochord, the
CC entire posterior region and the allantois, and die at about 10 days of
CC gestation.
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DR EMBL; X51683; CAA35985.1; -; mRNA.
DR CCDS; CCDS28383.1; -.
DR PIR; S08156; S08156.
DR RefSeq; NP_033335.1; NM_009309.2.
DR RefSeq; XP_011244504.1; XM_011246202.1.
DR AlphaFoldDB; P20293; -.
DR SMR; P20293; -.
DR DIP; DIP-61216N; -.
DR IntAct; P20293; 3.
DR STRING; 10090.ENSMUSP00000074236; -.
DR PhosphoSitePlus; P20293; -.
DR PaxDb; P20293; -.
DR PRIDE; P20293; -.
DR Antibodypedia; 925; 473 antibodies from 35 providers.
DR DNASU; 20997; -.
DR Ensembl; ENSMUST00000074667; ENSMUSP00000074236; ENSMUSG00000062327.
DR GeneID; 20997; -.
DR KEGG; mmu:20997; -.
DR UCSC; uc008ajq.1; mouse.
DR CTD; 20997; -.
DR MGI; MGI:98472; T.
DR VEuPathDB; HostDB:ENSMUSG00000062327; -.
DR eggNOG; KOG3585; Eukaryota.
DR GeneTree; ENSGT00940000157912; -.
DR HOGENOM; CLU_038303_1_1_1; -.
DR InParanoid; P20293; -.
DR OMA; RNDHKDM; -.
DR OrthoDB; 1201455at2759; -.
DR PhylomeDB; P20293; -.
DR TreeFam; TF106341; -.
DR BioGRID-ORCS; 20997; 0 hits in 2 CRISPR screens.
DR PRO; PR:P20293; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; P20293; protein.
DR Bgee; ENSMUSG00000062327; Expressed in embryonic post-anal tail and 54 other tissues.
DR ExpressionAtlas; P20293; baseline and differential.
DR Genevisible; P20293; MM.
DR GO; GO:0000785; C:chromatin; IDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:BHF-UCL.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:BHF-UCL.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IMP:MGI.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR GO; GO:0060349; P:bone morphogenesis; IGI:MGI.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; NAS:UniProtKB.
DR GO; GO:0060379; P:cardiac muscle cell myoblast differentiation; IMP:BHF-UCL.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:0071300; P:cellular response to retinoic acid; IEA:Ensembl.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; IMP:MGI.
DR GO; GO:0048706; P:embryonic skeletal system development; IMP:MGI.
DR GO; GO:0003007; P:heart morphogenesis; ISO:MGI.
DR GO; GO:0007498; P:mesoderm development; IMP:MGI.
DR GO; GO:0001707; P:mesoderm formation; IBA:GO_Central.
DR GO; GO:0007509; P:mesoderm migration involved in gastrulation; IMP:MGI.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IDA:BHF-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:BHF-UCL.
DR GO; GO:0001839; P:neural plate morphogenesis; IMP:MGI.
DR GO; GO:0001843; P:neural tube closure; IMP:MGI.
DR GO; GO:0030903; P:notochord development; IMP:MGI.
DR GO; GO:0014028; P:notochord formation; IMP:MGI.
DR GO; GO:0007341; P:penetration of zona pellucida; IMP:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:BHF-UCL.
DR GO; GO:0036342; P:post-anal tail morphogenesis; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0022414; P:reproductive process; IMP:MGI.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IDA:MGI.
DR GO; GO:0060395; P:SMAD protein signal transduction; IDA:MGI.
DR GO; GO:0001756; P:somitogenesis; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0001570; P:vasculogenesis; IMP:MGI.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR002070; TF_Brachyury.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 1.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00938; BRACHYURY.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..436
FT /note="T-box transcription factor T"
FT /id="PRO_0000184415"
FT DNA_BIND 51..219
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
SQ SEQUENCE 436 AA; 47440 MW; 1A0BDF9BA7FC2484 CRC64;
MSSPGTESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEESE LWLRFKELTN
EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVTADNH RWKYVNGEWV PGGKPEPQAP
SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGPQR
MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERNDHKDVM EEPGDCQQPG
YSQWGWLVPG AGTLCPPASS HPQFGGSLSL PSTHGCERYP ALRNHRSSPY PSPYAHRNSS
PTYADNSSAC LSMLQSHDNW SSLGVPGHTS MLPVSHNASP PTGSSQYPSL WSVSNGTITP
GSQTAGVSNG LGAQFFRGSP AHYTPLTHTV SAATSSSSGS PMYEGAATVT DISDSQYDTA
QSLLIASWTP VSPPSM
