TC1A_CAEEL
ID TC1A_CAEEL Reviewed; 273 AA.
AC P03934; Q23636;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Transposable element Tc1 transposase;
GN Name=tc1a; Synonyms=tca; ORFNames=T07D3.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6306578; DOI=10.1093/nar/11.12.4201;
RA Rosenzweig B., Liao L.W., Hirsh D.;
RT "Sequence of the C. elegans transposable element Tc1.";
RL Nucleic Acids Res. 11:4201-4209(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=2156234; DOI=10.1093/nar/18.4.895;
RA Schukkink R.F., Plasterk R.H.A.;
RT "TcA, the putative transposase of the C. elegans Tc1 transposon, has an N-
RT terminal DNA binding domain.";
RL Nucleic Acids Res. 18:895-900(1990).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS.
RX PubMed=7813449; DOI=10.1002/j.1460-2075.1994.tb06959.x;
RA Vos J.C., Plasterk R.H.A.;
RT "Tc1 transposase of Caenorhabditis elegans is an endonuclease with a
RT bipartite DNA binding domain.";
RL EMBO J. 13:6125-6132(1994).
CC -!- FUNCTION: Probably essential for transposable element Tc1
CC transposition. The insertion of Tc1 is the main cause of spontaneous
CC mutations. It is an endonuclease which can produce a single strand nick
CC at the 5'-end of the transposon. {ECO:0000269|PubMed:2156234,
CC ECO:0000269|PubMed:7813449}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:2156234,
CC ECO:0000269|PubMed:7813449}.
CC -!- MISCELLANEOUS: The N-terminal region of Tc1 transposase has strong
CC affinity for double and single-stranded DNA.
CC -!- SIMILARITY: Belongs to the transposase 5 family. {ECO:0000305}.
CC -!- CAUTION: Some sequenced Tc1 elements display an extra base T that would
CC give rise to another start point for Tc1 transposase. This could result
CC in a protein of 335 AA instead of 273 AA (displayed). {ECO:0000305}.
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DR EMBL; X01005; CAA25498.1; -; Genomic_DNA.
DR EMBL; FO081675; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A04520; QQKWTA.
DR AlphaFoldDB; P03934; -.
DR SMR; P03934; -.
DR InParanoid; P03934; -.
DR Proteomes; UP000001940; Chromosome II.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006313; P:transposition, DNA-mediated; IEA:InterPro.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR038717; Tc1-like_DDE_dom.
DR InterPro; IPR002492; Transposase_Tc1-like.
DR Pfam; PF13358; DDE_3; 1.
DR Pfam; PF01498; HTH_Tnp_Tc3_2; 1.
PE 3: Inferred from homology;
KW DNA integration; DNA recombination; DNA-binding; Endonuclease; Hydrolase;
KW Nuclease; Nucleus; Reference proteome; Transposable element.
FT CHAIN 1..273
FT /note="Transposable element Tc1 transposase"
FT /id="PRO_0000072444"
SQ SEQUENCE 273 AA; 31820 MW; 57C3A635CC8E90DB CRC64;
MDRNILRSAR EDPHRTATDI QMIISSPNEP VPSKRTVRRR LQQAGLHGRK PVKKPFISKK
NRMARVAWAK AHLRWGRQEW AKHIWSDESK FNLFGSDGNS WVRRPVGSRY SPKYQCPTVK
HGGGSVMVWG CFTSTSMGPL RRIQSIMDRF QYENIFETTM RPWALQNVGR GFVFQQDNDP
KHTSLHVRSW FQRRHVHLLD WPSQSPDLNP IEHLWEELER RLGGIRASNA DAKFNQLENA
WKAIPMSVIH KLIDSMPRRC QAVIDANGYA TKY
