TC32A_BRANA
ID TC32A_BRANA Reviewed; 320 AA.
AC A0A078IS66; P81259; Q7DM16;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 29-OCT-2014, sequence version 1.
DT 03-AUG-2022, entry version 27.
DE RecName: Full=Short-chain dehydrogenase TIC 32 A, chloroplastic {ECO:0000305};
DE EC=1.1.1.- {ECO:0000305};
DE AltName: Full=Pod-specific dehydrogenase SAC25 {ECO:0000303|PubMed:8111020};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 32 A {ECO:0000305};
DE Short=BnTIC32A {ECO:0000305};
GN Name=TIC32A {ECO:0000305};
GN Synonyms=BnaC04g53640D {ECO:0000312|EMBL:CDY52817.1},
GN SAC25 {ECO:0000303|PubMed:8111020};
GN ORFNames=GSBRNA2T00007891001 {ECO:0000312|EMBL:CDY52817.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Rafal; TISSUE=Pod;
RX PubMed=8111020; DOI=10.1007/bf00040589;
RA Coupe S.A., Taylor J.E., Isaac P.G., Roberts J.A.;
RT "Characterization of a mRNA that accumulates during development of oilseed
RT rape pods.";
RL Plant Mol. Biol. 24:223-227(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE, AND FUNCTION.
RA Isaac P.G., Roberts J.A., Coupe S.A.;
RT "CONTROL OF PLANT ABSCISSION AND POD DEHISCENCE.";
RL Patent number WO9423043, 13-OCT-1994.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh;
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts (By
CC similarity). Maybe involved in pod abscission or dehiscence (pod
CC shatter) (Probable). {ECO:0000250|UniProtKB:Q6RVV4,
CC ECO:0000305|PubMed:8111020, ECO:0000305|Ref.2}.
CC -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:Q6RVV4}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000250|UniProtKB:Q6RVV4}.
CC -!- TISSUE SPECIFICITY: Expressed in the dehiscence zone of developing
CC pods. {ECO:0000269|PubMed:8111020}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during pod development.
CC {ECO:0000269|PubMed:8111020}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; X74225; CAB58175.1; -; mRNA.
DR EMBL; A40116; CAA02476.1; -; Unassigned_DNA.
DR EMBL; LK033133; CDY52817.1; -; Genomic_DNA.
DR PIR; S42651; S42651.
DR RefSeq; NP_001302998.1; NM_001316069.1.
DR AlphaFoldDB; A0A078IS66; -.
DR SMR; A0A078IS66; -.
DR EnsemblPlants; CDY52817; CDY52817; GSBRNA2T00007891001.
DR GeneID; 106397857; -.
DR Gramene; CDY52817; CDY52817; GSBRNA2T00007891001.
DR KEGG; bna:106397857; -.
DR OMA; NRPVVGW; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR ExpressionAtlas; A0A078IS66; baseline.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Calmodulin-binding; Chloroplast; Membrane; NADP; Oxidoreductase; Plastid;
KW Plastid inner membrane; Protein transport; Reference proteome; Transport.
FT CHAIN 1..320
FT /note="Short-chain dehydrogenase TIC 32 A, chloroplastic"
FT /id="PRO_0000448489"
FT REGION 301..317
FT /note="Interaction with calmodulin"
FT /evidence="ECO:0000250|UniProtKB:Q6RVV4"
FT ACT_SITE 196
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P00334"
FT BINDING 40..46
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q8KES3"
FT BINDING 92..93
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q8KES3"
FT BINDING 119
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q8KES3"
FT BINDING 140
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250|UniProtKB:Q8KES3"
FT BINDING 174
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q8KES3"
FT CONFLICT 300
FT /note="I -> T (in Ref. 1; CAB58175 and 2; CAA02476)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 34923 MW; B27DB6BE5192434C CRC64;
MEIYGMVTGK AGKSGYGSAS TAEDVTHSID AKHLTAIITG GTSGIGLEAA RVLGMRGAHV
IIASRNTKAA NDSKEMILQM YPNARIDCLQ LDLSSIKSVR SFIHQFLALN VPLNILINNA
GVMFCPFQLS EDGIESQFAT NHIGHFLLTN LLLDKMKSSA RESGIEGRIV NLSSIAHTYT
YTEGIMFDYI NDPDRYSEKK AYGQSKLANL LHSNALSRKL QEEGVNITIN SVHPGLITTN
LFRHSGLGMA VLKAMSFFLW KNIPQGAATT CYVALHPDLK DVTGKYFADC NVTTPSNFAI
DTTLADKLWD FSIKLVESLP
