ID   TC3A_CAEEL              Reviewed;         329 AA.
AC   P34257;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Transposable element Tc3 transposase;
GN   Name=tc3a; ORFNames=B0303.5;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=1538779; DOI=10.1038/356037a0;
RA   Sulston J., Du Z., Thomas K., Wilson R., Hillier L., Staden R.,
RA   Halloran N., Green P., Thierry-Mieg J., Qiu L., Dear S., Coulson A.,
RA   Craxton M., Durbin R., Berks M., Metzstein M., Hawkins T., Ainscough R.,
RA   Waterston R.;
RT   "The C. elegans genome sequencing project: a beginning.";
RL   Nature 356:37-41(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [3]
RP   FUNCTION.
RX   PubMed=8389698;
RA   van Luenen H.G.A.M., Colloms S.D., Plasterk R.H.A.;
RT   "Mobilization of quiet, endogenous Tc3 transposons of Caenorhabditis
RT   elegans by forced expression of Tc3 transposase.";
RL   EMBO J. 12:2513-2520(1993).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 2-52 IN COMPLEX WITH DNA.
RX   PubMed=9312061; DOI=10.1093/emboj/16.19.6044;
RA   van Pouderoyen G., Ketting R.F., Perrakis A., Plasterk R.H.A., Sixma T.K.;
RT   "Crystal structure of the specific DNA-binding domain of Tc3 transposase of
RT   C.elegans in complex with transposon DNA.";
RL   EMBO J. 16:6044-6054(1997).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS) OF 1-135 IN COMPLEX WITH DNA.
RX   PubMed=15304566; DOI=10.1093/nar/gkh770;
RA   Watkins S., van Pouderoyen G., Sixma T.K.;
RT   "Structural analysis of the bipartite DNA-binding domain of Tc3 transposase
RT   bound to transposon DNA.";
RL   Nucleic Acids Res. 32:4306-4312(2004).
CC   -!- FUNCTION: Binds specifically to the terminal nucleotides of the TC3
CC       inverted repeat. Its expression results in frequent excision and
CC       transposition of endogenous TC3 elements. TC3 transposase acts by
CC       making double strand breaks at the ends of TC3 element. The excised
CC       element would then be inserted into a target sequence.
CC       {ECO:0000269|PubMed:8389698}.
CC   -!- SUBUNIT: Homodimer or homotetramer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the transposase 5 family. {ECO:0000305}.
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DR   EMBL; FO080163; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; S27787; S27787.
DR   PDB; 1TC3; X-ray; 2.45 A; C=2-52.
DR   PDB; 1U78; X-ray; 2.69 A; A=1-135.
DR   PDBsum; 1TC3; -.
DR   PDBsum; 1U78; -.
DR   AlphaFoldDB; P34257; -.
DR   SMR; P34257; -.
DR   InParanoid; P34257; -.
DR   EvolutionaryTrace; P34257; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   DisProt; DP02799; -.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   IDEAL; IID50076; -.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR038717; Tc1-like_DDE_dom.
DR   InterPro; IPR025898; Tc3_transposase_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF13358; DDE_3; 1.
DR   Pfam; PF11427; HTH_Tnp_Tc3_1; 1.
DR   SUPFAM; SSF46689; SSF46689; 2.
PE   1: Evidence at protein level;
KW   3D-structure; DNA integration; DNA recombination; DNA-binding; Nucleus;
KW   Reference proteome; Transposable element.
FT   CHAIN           1..329
FT                   /note="Transposable element Tc3 transposase"
FT                   /id="PRO_0000072445"
FT   DNA_BIND        2..135
FT   HELIX           9..20
FT                   /evidence="ECO:0007829|PDB:1TC3"
FT   HELIX           25..32
FT                   /evidence="ECO:0007829|PDB:1TC3"
FT   HELIX           36..44
FT                   /evidence="ECO:0007829|PDB:1TC3"
FT   TURN            47..50
FT                   /evidence="ECO:0007829|PDB:1TC3"
FT   HELIX           63..75
FT                   /evidence="ECO:0007829|PDB:1U78"
FT   HELIX           80..86
FT                   /evidence="ECO:0007829|PDB:1U78"
FT   HELIX           93..102
FT                   /evidence="ECO:0007829|PDB:1U78"
SQ   SEQUENCE   329 AA;  38253 MW;  F7421854CE11C6DB CRC64;
     MPRGSALSDT ERAQLDVMKL LNVSLHEMSR KISRSRHCIR EYLKDPVSYG TSKRAPRRKA
     LSVRDERNVI RAASNSCKTA RDIRNELQLS ASKRTILNVI KRSGVIVRQK LRPAPLLSAD
     HKLKRLEFAK NNMGTNWSKV VFSDEKKFNL DGPDGCRYYW RDLRKEPMVF SRRNFGGGTV
     MVWGAFTEKK KLEIQFVSSK MNSTDYQNVL ELELSKYLRH YSRKDFRFQQ DNATIHVSNS
     TRDYFKLKKI NLLDWPARSP DLNPIENLWG ILVRIVYAQN KTYPTVASLK QGILDAWKSI
     PDNQLKSLVR SMEDRLIEII RTQGNPINY