TCA17_YEAST
ID TCA17_YEAST Reviewed; 152 AA.
AC P32613; D3DLK2; Q6Q561;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=TRAPP-associated protein TCA17;
DE AltName: Full=17 kDa TRAPP complex-associated protein;
GN Name=TCA17; OrderedLocusNames=YEL048C; ORFNames=SYGP-ORF36;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, INTERACTION WITH THE TRAPP II COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=19220810; DOI=10.1111/j.1600-0854.2009.00895.x;
RA Montpetit B., Conibear E.;
RT "Identification of the novel TRAPP associated protein Tca17.";
RL Traffic 10:713-723(2009).
RN [6]
RP FUNCTION, AND INTERACTION WITH THE TRAPP II COMPLEX.
RX PubMed=19416478; DOI=10.1111/j.1600-0854.2009.00906.x;
RA Scrivens P.J., Shahrzad N., Moores A., Morin A., Brunet S., Sacher M.;
RT "TRAPPC2L is a novel, highly conserved TRAPP-interacting protein.";
RL Traffic 10:724-736(2009).
CC -!- FUNCTION: Required, together with the TRAPP II subunit TRS33, for TRAPP
CC II complex assembly or stability, and for proper Golgi localization of
CC TRAPP and the Rab GTPase YPT31. {ECO:0000269|PubMed:19220810,
CC ECO:0000269|PubMed:19416478}.
CC -!- SUBUNIT: Interacts with the TRAPP II complex; TRAPP II subunits TRS33
CC and TRS65 are required for this interaction.
CC {ECO:0000269|PubMed:19220810, ECO:0000269|PubMed:19416478}.
CC -!- INTERACTION:
CC P32613; Q03660: TRS130; NbExp=7; IntAct=EBI-22370, EBI-19461;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000269|PubMed:19220810}.
CC -!- MISCELLANEOUS: Present with 8660 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TRAPP small subunits family. Sedlin
CC subfamily. {ECO:0000305}.
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DR EMBL; U18779; AAB64994.1; -; Genomic_DNA.
DR EMBL; AY558442; AAS56768.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07606.1; -; Genomic_DNA.
DR PIR; S30829; S30829.
DR RefSeq; NP_010866.1; NM_001178863.1.
DR PDB; 3PR6; X-ray; 1.80 A; A=1-152.
DR PDB; 7E2C; EM; 4.18 A; A=1-152.
DR PDB; 7E2D; EM; 3.71 A; A=1-152.
DR PDB; 7E8S; EM; 4.36 A; A/L=1-152.
DR PDB; 7E8T; EM; 3.80 A; A=1-152.
DR PDB; 7E93; EM; 6.54 A; A/L=1-152.
DR PDB; 7E94; EM; 4.67 A; A/L=1-152.
DR PDB; 7EA3; EM; 4.31 A; A/N=1-152.
DR PDBsum; 3PR6; -.
DR PDBsum; 7E2C; -.
DR PDBsum; 7E2D; -.
DR PDBsum; 7E8S; -.
DR PDBsum; 7E8T; -.
DR PDBsum; 7E93; -.
DR PDBsum; 7E94; -.
DR PDBsum; 7EA3; -.
DR AlphaFoldDB; P32613; -.
DR SMR; P32613; -.
DR BioGRID; 36682; 75.
DR ComplexPortal; CPX-1939; TRAPPII protein complex.
DR DIP; DIP-5288N; -.
DR IntAct; P32613; 14.
DR STRING; 4932.YEL048C; -.
DR MaxQB; P32613; -.
DR PaxDb; P32613; -.
DR PRIDE; P32613; -.
DR EnsemblFungi; YEL048C_mRNA; YEL048C; YEL048C.
DR GeneID; 856663; -.
DR KEGG; sce:YEL048C; -.
DR SGD; S000000774; TCA17.
DR VEuPathDB; FungiDB:YEL048C; -.
DR eggNOG; ENOG502S4FS; Eukaryota.
DR HOGENOM; CLU_097630_1_0_1; -.
DR InParanoid; P32613; -.
DR OMA; CKCNPFV; -.
DR BioCyc; YEAST:G3O-30166-MON; -.
DR PRO; PR:P32613; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P32613; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:SGD.
DR GO; GO:1990071; C:TRAPPII protein complex; IDA:SGD.
DR GO; GO:0034498; P:early endosome to Golgi transport; IMP:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEA:InterPro.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IC:ComplexPortal.
DR GO; GO:0065003; P:protein-containing complex assembly; IMP:SGD.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IC:ComplexPortal.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR006722; Sedlin.
DR Pfam; PF04628; Sedlin_N; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Golgi apparatus; Reference proteome.
FT CHAIN 1..152
FT /note="TRAPP-associated protein TCA17"
FT /id="PRO_0000202605"
FT CONFLICT 2
FT /note="S -> T (in Ref. 3; AAS56768)"
FT /evidence="ECO:0000305"
FT STRAND 6..11
FT /evidence="ECO:0007829|PDB:3PR6"
FT STRAND 17..21
FT /evidence="ECO:0007829|PDB:3PR6"
FT HELIX 33..48
FT /evidence="ECO:0007829|PDB:3PR6"
FT STRAND 57..60
FT /evidence="ECO:0007829|PDB:3PR6"
FT STRAND 64..69
FT /evidence="ECO:0007829|PDB:3PR6"
FT STRAND 72..78
FT /evidence="ECO:0007829|PDB:3PR6"
FT TURN 80..82
FT /evidence="ECO:0007829|PDB:3PR6"
FT STRAND 85..91
FT /evidence="ECO:0007829|PDB:3PR6"
FT HELIX 92..94
FT /evidence="ECO:0007829|PDB:3PR6"
FT HELIX 99..120
FT /evidence="ECO:0007829|PDB:3PR6"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:3PR6"
FT HELIX 130..145
FT /evidence="ECO:0007829|PDB:3PR6"
FT HELIX 149..152
FT /evidence="ECO:0007829|PDB:3PR6"
SQ SEQUENCE 152 AA; 17354 MW; F7AF8E6EC34F193D CRC64;
MSLRPCFVSL IDESDKPILI YVPNEAENEM NDVLKYNVLS NISLDYFESA LVEWHSLDSK
PLLKSIFQLE GVSVFAMLIK QTGLKIVIGF EQKSLSGADD EFEAINQIFE TVRKIYIRVK
CNPLLVSGDE KSIIKSLERK FDELFISTEV EL
